Center for Synthetic Regulatory Genomics

合成监管基因组学中心

基本信息

批准号：
10392966
负责人：
Jef D BOEKE
金额：
$ 197.25万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-06-12 至 2023-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10392966
关键词：
Affect Animal Model Architecture Attention Automation Binding Biotechnology CRISPR/Cas technology Cell Nucleus Chromatin Code Communities Computer Models Cultured Cells DNA DNA biosynthesis DNase I hypersensitive sites sequencing Data Deoxyribonuclease I Development Disease Elements Enhancers Event Explosion Exposure to Fostering Gene Expression Gene Expression Regulation Genes Genetic Recombination Genome Genomics Haplotypes Hi-C Human Human Genetics Hypersensitivity Immunoglobulin Constant Region Individual Introns Investigation Length Libraries Library Surveys Link Maps Methods Modeling Molecular Conformation Monitor Nuclear Operative Surgical Procedures Play Positioning Attribute Reagent Regulation Regulatory Element Replication Origin Reporting Research Personnel Residual state Resolution Role Series Site Specific qualifier value Students Surveys Technology Testing Untranslated RNA Variant Work Yeasts combinatorial dark matter design gene function gene therapy genome wide association study genome-wide genomic locus human genomics improved interest member new technology novel outreach program personalized medicine programs public health relevance summer program synthetic construct three dimensional structure tool trait transcription factor transcriptome sequencing translational applications undergraduate student

项目摘要

Modified Project Summary/Abstract Section The Center for Synthetic Regulatory Genomics (SyRGe) is tasked with development and application of revolutionary technology for making dramatic, coordinated changes to extensive gene loci, which will enable broad investigation of the function of regulatory sequences and foster translational applications to biotechnology, personalized medicine and gene therapy. Specifically, we will (i) design and synthesize over 1000 >100kbp constructs probing regulatory function at a carefully selected set of genes; (ii) develop a pipeline for their single-copy integration at any desired site genome-wide; (iii) survey libraries for effects on gene expression, chromatin features, and nuclear architecture; and (iv) develop global computational models to predict regulatory effects of sequence variation on gene expression. The Center will dramatically supersede present and predicted technologies for manipulation and assessment of regulatory genome function. This Center also incorporates a unique and highly successful outreach program whereby undergraduates from diverse backgrounds play a crucial role in genome assembly, as well as a new Fellows program to expose researchers and students from other fields to transformative new technology and facilitate its promulgation throughout the larger human genetics and genomics communities.

修改的项目摘要/摘要部分合成调节基因组学中心（Syrge）的任务是开发和应用革命性技术，以对广泛的基因基因座进行戏剧性，协调的更改，这将能够广泛研究调节序列的功能，并促进对生物技术，个性化医学和基因治疗的转化应用功能。具体而言，我们将（i）设计和合成超过1000> 100kbp的构造，以一组精心选择的基因探测调节函数；（ii）在任何所需的位点基因组中开发了其单拷贝整合的管道；（iii）对基因表达，染色质特征和核结构的影响的调查文库；（iv）开发全球计算模型，以预测序列变化对基因表达的调节作用。该中心将极大地取代当前和预测调节基因组功能的操纵和评估的技术。该中心还纳入了一个独特而成功的外展计划，从不同背景的大学生中的大学生在基因组组装中起着至关重要的作用，以及一个新的研究员计划，可以使研究人员和学生从其他领域揭露新技术，并在整个较大的人类遗传学社区中促进其在整个较大的人类遗传学社区中的颁布。