Core B - Retrotransposon Genomics, Technology and Analysis Core

核心 B - 逆转录转座子基因组学、技术和分析核心

基本信息

批准号：
10333659
负责人：
Jef D BOEKE
金额：
$ 50.68万
依托单位：
BROWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2026-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10333659
关键词：
Affinity Chromatography Aging Alzheimer&apos s Disease Animal Model Animals Area Biological Biological Assay Biology Cell Aging Cells ChIP-seq Clinical Collaborations Complementary DNA Complex Computer Analysis Computing Methodologies DNA Data Data Set Dementia Detection Disease Drosophila genus Elements Engineering Environment Eukaryota Event Genes Genomics Germ Lines Goals Grant Hybrids Imaging technology Individual Interphase Cell L1 Elements Lead Life Cycle Stages Location Mass Spectrum Analysis Measurement Measures Methods Mitotic Modeling Monitor Mus Nerve Degeneration Neurodegenerative Disorders Organism Participant Property Proteins Proteomics RNA immunoprecipitation sequencing Reporter Reporting Retrotransposition Retrotransposon Reverse Transcription Role Sampling Sequence Analysis Stimulator of Interferon Genes System Technology Tissues Transcript Transcriptional Regulation Translations Work aging brain cell type computerized tools demethylation design design and construction fascinate gene product genomic tools imaging modality improved in vivo insight interest mouse model novel promoter response single cell sequencing single molecule single-cell RNA sequencing technological innovation tool transcriptome sequencing transcriptomics

项目摘要

Core Summary Core B will provide cutting edge tools to measure retrotransposition in multicellular eukaryotes in the context of aging paradigms with a special focus on Alzheimer’s disease and related disorders. In addition to providing appropriate existing assays and mouse models to all of the participants, the core will also develop improved retrotransposition reporters and genomics tools to enable the detection and mapping of retrotransposition events in the various experimental systems under study. The Core will also provide “Driver systems” that lead to high level expression of these elements in both cell systems and animals. Finally, the core will provide cutting edge sequencing and computational tools to analyze both retrotransposition itself and the aberrant forms of LINE-1 expression and reverse transcription observed in aging cells and organisms.

核心总结核心 B 将提供尖端工具来测量逆转录转座衰老范式背景下的多细胞真核生物，特别关注阿尔茨海默病和相关疾病。向所有参与者提供现有的测定和小鼠模型，核心还将开发改进的逆转录转座生产者和基因组学工具，使各种实验中逆转录转座事件的检测和绘图正在研究的系统还将提供“驱动系统”。最后，这些元素在细胞系统和动物中的高水平表达。该核心将提供尖端的测序和计算工具来分析逆转录转座本身和 LINE-1 表达的异常形式在衰老细胞和生物体中观察到逆转录。