The Effects of Chemical Repellents on Olfactory Receptors of the Aedes aegypti Mosquito in Response to Human Odorants – the Mechanism Underlying Host-seeking and Blood-feeding Behaviors

化学驱避剂对埃及伊蚊嗅觉受体响应人类气味的影响——寻找宿主和吸血行为的机制

• 批准号: 10373255
• 负责人: NANNAN LIU
• 金额: $ 7.48万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-11-12 至 2023-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10373255
• 关键词: Acetylcholinesterase; Aedes; Affect; Behavior; Biological; Blood; Central Africa; Cessation of life; Chemicals; Country; Culicidae; Cytochrome P450; Detection; Development; Disease; Disease Outbreaks; Electrodes; Exhibits; Exposure to; Feeding behaviors; Female; Food; Funding; Gene Expression; Gene Proteins; Goals; Human; Incidence; Industry; Insect Repellents; Insecta; Insecticide Resistance; Insecticides; Knock-in; Knock-out; Malaria; Measures; Molecular; Mosquito-borne infectious disease; Neurons; Odors; Olfactory Pathways; Olfactory Receptor Neurons; Pathway interactions; Population; Public Health; Reporting; Research; Resistance; Smell Perception; System; Techniques; Terpenes; Testing; Toxic effect; United States National Institutes of Health; Voltage-Clamp Technics; Xenopus oocyte; desensitization; feeding; knock-down; mosquito reproduction; olfactory receptor; prevent; pyrethroid; response; transmission process; vector mosquito; Acetylcholinesterase; Aedes; Affect; Behavior; Biological; Blood; Central Africa; Cessation of life; Chemicals; Country; Culicidae; Cytochrome P450; Detection; Development; Disease; Disease Outbreaks; Electrodes; Exhibits; Exposure to; Feeding behaviors; Female; Food; Funding; Gene Expression; Gene Proteins; Goals; Human; Incidence; Industry; Insect Repellents; Insecta; Insecticide Resistance; Insecticides; Knock-in; Knock-out; Malaria; Measures; Molecular; Mosquito-borne infectious disease; Neurons; Odors; Olfactory Pathways; Olfactory Receptor Neurons; Pathway interactions; Population; Public Health; Reporting; Research; Resistance; Smell Perception; System; Techniques; Terpenes; Testing; Toxic effect; United States National Institutes of Health; Voltage-Clamp Technics; Xenopus oocyte; desensitization; feeding; knock-down; mosquito reproduction; olfactory receptor; prevent; pyrethroid; response; transmission process; vector mosquito

Project Summary/Abstract The long-term goal of the proposed research is to develop effective, alternative chemical repellents that counter mosquito host-seeking and blood-feeding behaviors by disrupting the mosquito’s human odor- detection mechanism. The information gained in this proposal will inform the development of chemical repellents that by-pass known mechanisms of insecticide resistance in order to reduce the incidence of mosquito-borne diseases and to protect public health. When seeking blood meals, mosquitoes rely on their olfactory system to detect chemical odorants emitted by their human hosts [8]; these human odorants assist in the t ransmission of mosquito-related diseases to humans. Using chemical repellents that disrupt the odor-detection mechanisms in mosquitoes may be a viable alternative strategy to current insecticides, such as DEET (Section A.3), for desensitizing the mosquito’s ability to smell humans. A better understanding is needed of the mechanisms governing the interactions among chemical repellents, host odorants, and the response of olfactory receptors of mosquitoes to enable the development of new repellent alternatives that will desensitize the mosquito to humans, thus interfering with the mosquito recognizing its human host. In preliminary studies, we investigated the interactions of 48 repellent chemicals with the olfactory receptor neurons (ORNs) of the Aedes aegypti mosquito, and identified several terpene/terpenoid compounds that exhibit inhibitory effects on mosquito ORNs [40]. We have further characterized the responses of the ORN to 103 compounds and discovered that certain human odorants elicit decreased responses after blood feeding, suggesting these human odorants are involved in Aedes aegypti host-seeking behaviors [21]. We hypothesize that chemical repellents exhibiting inhibitory effects (inhibitory chemical repellents) may interact with human odorants, resulting in the disruption of the mosqutoes’ olfactory recognition of human hosts and in diminished responses to them when mosquitoes are seeking feeding hosts. The proposed research objective is to determine the chemical repellents that affect a mosquito’s olfactory sense in order to prevent it from finding a human target or host. The proposed aims are to: 1) characterize the responses of Ae. aegypti ORs to inhibitory chemical repellents and to human odorants that may be involved in Ae. aegypti host-seeking and blood-feeding and 2) determine the competition effects of inhibitory chemical repellents and human odorants on Ae. aegypti ORs.

项目摘要/摘要 拟议研究的长期目标是开发有效的替代化学复制剂， 通过破坏蚊子的人类odo- 检测机制。本提案中获得的信息将为化学的开发提供信息 复制副通知的杀虫剂抗性机制，以减少 蚊子传播疾病并保护公共卫生。 当寻求血液餐时，蚊子依靠其嗅觉系统来检测化学气味 由他们发出 人类寄主[8]；这些人类气味有助于 t 与蚊子相关的运行 对人类的疾病。使用破坏蚊子中气味检测机制的化学复制剂可能 成为当前杀虫剂的可行替代策略，例如DEET（A.3节），以使脱敏 蚊子闻到人类的能力。需要更好地理解管理的机制 化学驱蚊剂，宿主气味和蚊子嗅觉受体的反应之间的相互作用 为了使新的驱虫剂的替代品的发展将蚊子脱敏，从而使人脱敏 干涉蚊子认识到其人类宿主。 在初步研究中，我们研究了48种驱虫化学物质与嗅觉的相互作用 埃及埃及蚊子的受体神经元（ORNS），并鉴定出几种萜烯/萜类化合物 对蚊子的抑制作用存在[40]。我们进一步表征了ORN的响应 至103种化合物，并发现某些人类气味会在献血后引起反应下降， 暗示这些人类的气味参与了埃及埃及宿主寻求行为的行为[21]。我们假设 表现出抑制作用（抑制性化学复制剂）的化学复制剂可能与人相互作用 气味，导致Mosqutoes对人类宿主的嗅觉识别的破坏，并减少 当蚊子寻求喂养宿主时，对它们的反应。 拟议的研究目标是确定影响蚊子的化学驱虫剂 嗅觉是为了防止其找到人类目标或宿主。拟议的目的是：1） 表征AE的响应。埃及对抑制性化学驱虫剂和人类气味 可能参与了AE。埃及寻求宿主和喂血和2）确定竞争的影响 AE上的抑制性化学驱虫剂和人类气味。埃及。