National Center for Microscopy and Imaging Research: A BRAIN Technology Integration and Dissemination Resource

国家显微镜和成像研究中心：大脑技术集成和传播资源

基本信息

批准号：
10334513
负责人：
Mark H Ellisman
金额：
$ 112.88万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-02-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10334513
关键词：
3-Dimensional Achievement Actins Animal Model BRAIN initiative Behavior Biomedical Technology Brain Brain imaging Chemicals Communities Community Outreach Complement Complex Data Analyses Data Set Disease Electron Microscopy Elements Endoplasmic Reticulum Extracellular Matrix Genetic Goals Histologic Image Imaging Device Imaging Techniques Imaging technology Immersion In Situ Information Technology Investigation Label Learning Light Maps Measures Methods Microscopy Mitochondria Molecular Molecular Probes National Center for Research Resources National Institute of General Medical Sciences Nerve Tissue Neurons Neurosciences Physiological Population Preparation Process Recovery Research Research Personnel Research Project Grants Resolution Resource Sharing Resources Roentgen Rays Role Sampling Scanning Electron Microscopy Specimen Stains Structure Structure-Activity Relationship Subcellular Anatomy Techniques Technology Training Ultramicrotomy Vertebral column Vesicle Visualization Work X ray microscopy base brain cell brain research brain tissue cell type computerized tools connectome cost cryogenics cyber infrastructure data acquisition data dissemination data management data sharing deep learning imaging platform insight instrument instrumentation interest large scale data light microscopy microscopic imaging multimodal data multimodality nano novel operation preservation reconstruction relating to nervous system spatiotemporal success synaptic function tomography tool voltage

项目摘要

PROJECT SUMMARY/ABSTRACT This project aims to disseminate validated technologies and resources of the National Center for Microscopy and Imaging Research (NCMIR) at UC San Diego to advance the completion of strategic goals of the BRAIN Initiative. The proposed technology integration and dissemination resource will provide the neuroscience community with technical help to obtain and manage large scale data, broadening the access to and incorporation of high throughput multiscale imaging tools and leading-edge analysis strategies in their studies. Richly integrated resources will be offered, including molecular probes, imaging platforms and data analysis tools certain to enable and hasten brain research. The types of research projects we will support, include: 1) investigations requiring the traversal of spatiotemporal scales to reveal new insight and understanding of specific neural populations; 2) investigations which aim to mark and track neuronal processes and/or visualize targeted connectomes of local circuits within large volumes of nervous tissue in multiple animal models; 3) projects which seek to perform nano-histological assessment of cellular and subcellular level alterations associated with learning, physiological state, or disease; and 4) projects performing higher resolution 3D morphometric analysis of subcellular underpinnings of function, with particular emphasis on synaptic function as influenced by subcellular constituents. This new center will leverage an administrative framework and information technology cyberinfrastructure which is already in place and has been refined over many years. In addition to providing a management structure, this framework includes a community outreach, project review, selection, and onboarding process refined and long practiced by the PI (and the assembled team). Additional mechanisms for tool/technology dissemination and training, data management and sharing, and mechanisms for cost-recovery and long-term sustainability will also be leveraged and expanded so as to maximize the reach and impact of our technologies and resources.

项目概要/摘要该项目旨在传播国家显微镜中心的经过验证的技术和资源加州大学圣地亚哥分校和影像研究 (NCMIR) 推进 BRAIN 战略目标的完成倡议。拟议的技术集成和传播资源将为神经科学提供社区提供技术帮助来获取和管理大规模数据，扩大对数据的访问和在他们的研究中结合了高通量多尺度成像工具和前沿分析策略。提供丰富的整合资源，包括分子探针、成像平台和数据分析工具肯定会促进并加速大脑研究。我们将支持的研究项目类型包括：1) 需要穿越时空尺度的调查，以揭示对特定事物的新见解和理解神经群体； 2）旨在标记和跟踪神经元过程和/或可视化目标的研究多种动物模型中大量神经组织内局部回路的连接组； 3）项目寻求对与相关的细胞和亚细胞水平变化进行纳米组织学评估学习、生理状态或疾病； 4) 执行更高分辨率 3D 形态分析的项目亚细胞功能基础，特别强调突触功能受亚细胞成分。这个新中心将利用行政框架和信息技术网络基础设施已经到位，并经过多年的完善。除了提供管理结构，该框架包括社区外展、项目审查、选择和入职由 PI（和组建的团队）完善和长期实践的流程。附加机制工具/技术传播和培训、数据管理和共享以及成本回收机制长期可持续性也将得到利用和扩大，以最大限度地扩大我们的影响力和影响力技术和资源。