Role of Water in Dental Hard Tissues

水在牙齿硬组织中的作用

• 批准号: 10333042
• 负责人: Shan Yang
• 金额: $ 9.74万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10333042
• 关键词: Aging; Biological; Biological Markers; Chemicals; Communities; Dental; Dental Enamel; Dental Research; Dental caries; Dentin; Development; Devices; Diagnosis; Evaluation; Exposure to; Failure; Fatigue; Fluorescence; Functional disorder; High temperature of physical object; Hydration status; Lead; Lesion; Magnetism; Measurement; Measures; Mechanics; Metals; Methods; Minerals; Noise; Normal tissue morphology; Nuclear; Nuclear Magnetic Resonance; Odontogenesis; Pathology; Performance; Pilot Projects; Preparation; Prevention; Property; Publishing; Raman Spectrum Analysis; Reporting; Research; Role; Sampling; Scanning; Shapes; Signal Transduction; Spectrometry; Spottings; Structure; System; Testing; Time; Tissues; Tooth Diseases; Tooth structure; Variant; Water; absorption; base; clinical practice; demineralization; detector; diagnostic value; infrared spectroscopy; insight; interest; light scattering; mechanical properties; microCT; preference; success; tool

Summary Water is one of the three major constituents of dental hard tissues, but the structure of water and how water interacts with other components in dental hard tissues during teeth formation, degradation, and lesion remain unclear. Nuclear magnetic spectrometry (NMR) and infrared absorption (IR) are the methods that can directly probe water from biological tissues. However, their performances are limited because NMR only measures the average effect of the sample, while IR requires special preparations to tissues like teeth and could alter the properties of the tissues. Recently, we demonstrated that fluorescence compressed Raman spectroscopy has the capability of probing water in dental hard tissues and showed that the spectral profiles of water are dramatically different between dentin and enamel. The profiles varied even within enamel at different testing spots. Those results indicate that Raman spectroscopy may provide additional information about the role of water in dental hard tissues with details that may impact the understanding of the mechanisms of dental problems. The major aim of the proposed project is to use Raman spectroscopy to identify how the changes in water content and structure in dental hard tissues may correlate with dental tissue pathologies. Aim 1 will determine the best available Raman spectroscopy configurations for probing water contents in dental hard tissues. Three common fluorescence suppression approaches, i.e., near-infrared excitation combining InGaAs detector, UV resonance Raman, and time-gated Raman spectroscopy will be compared to determine how the signal to noise ratio of the water-related Raman signals can be maximumly enhanced. We expect to increase the system sensitivity by one order or more. Aim 2 will investigate the changes in water amount and structure changes reflected by Raman spectroscopy and identify if these changes are correlated with other chemical/physical changes in dental hard tissues. Teeth with various degrees of demineralization and decomposing of organics will be prepared to show deferent levels of chemical compositional changes. Raman spectra will be acquired from these simulated samples and used as references for probing compositional variations in teeth with naturally developed caries. Raman spectroscopy will also be combined with micro-mechanical testing to identify correlations between water contents and structure change and mechanical property changes.

概括 水是牙齿硬组织的三大组成部分之一，但水的结构及其形成方式 在牙齿形成、退化和病变过程中，水与牙齿硬组织中的其他成分相互作用 仍不清楚。核磁光谱 (NMR) 和红外吸收 (IR) 是可以 直接探测生物组织中的水。然而，它们的性能受到限制，因为核磁共振仅 测量样品的平均效果，而红外需要对牙齿等组织进行特殊准备，并且可能 改变组织的特性。最近，我们证明了荧光压缩拉曼光谱 具有探测牙齿硬组织中水的能力，并表明水的光谱分布是 牙本质和牙釉质之间存在显着差异。在不同的测试中，甚至牙釉质内部的轮廓也有所不同 斑点。这些结果表明拉曼光谱可以提供有关作用的更多信息 牙齿硬组织中的水，其细节可能会影响对牙齿机制的理解 问题。该项目的主要目标是利用拉曼光谱来确定 牙齿硬组织中水含量和结构的变化可能与牙齿组织相关 病理学。目标 1 将确定最佳可用拉曼光谱配置 探测牙齿硬组织中的水含量。三种常见的荧光抑制方法，即 近红外激发结合 InGaAs 探测器、紫外共振拉曼和时间选通拉曼光谱 将进行比较以确定与水相关的拉曼信号的信噪比如何 最大限度地增强。我们期望将系统灵敏度提高一个数量级或更多。目标2将 研究拉曼光谱反映的水量变化和结构变化 并确定这些变化是否与牙科硬质中的其他化学/物理变化相关 组织。具有不同程度脱矿和有机物分解的牙齿将被准备好展示 不同程度的化学成分变化。将从这些模拟样品中获取拉曼光谱 并用作探测自然形成龋齿的牙齿成分变化的参考。拉曼 光谱学还将与微观机械测试相结合，以确定水之间的相关性 含量和结构变化以及机械性能变化。