Shared Resource: Proteomics & Metabolomics

共享资源：蛋白质组学

• 批准号: 10319029
• 负责人: Philip R Gafken
• 金额: $ 37.48万
• 依托单位: FRED HUTCHINSON CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10319029
• 关键词: Analytical Biochemistry; Analytical Chemistry; Area; Awareness; Bile Acids; Bioinformatics; Biological; Biological Assay; Cancer Biology; Cancer Center Support Grant; Cancer Patient; Chromatography; Clinical; Coenzymes; Consult; Consultations; Data Analyses; Development; Digestion; Educational Materials; Educational workshop; Enzymes; Equipment; Experimental Designs; Feedback; Fractionation; Future; Gas Chromatography; Gel; General Population; Grant; High Pressure Liquid Chromatography; Human Resources; Individual; Informatics; Infrastructure; Isotopes; Lipids; Liquid substance; Major Histocompatibility Complex; Malignant Neoplasms; Mass Spectrum Analysis; Measures; Metabolic; Methodology; Methods; Mission; Modernization; Modification; Molecular; NMR Spectroscopy; Nuclear Magnetic Resonance; Pathway Analysis; Peptides; Policies; Post-Translational Protein Processing; Preparation; Process; Productivity; Proteins; Proteomics; Proteomics Shared Resource; Research; Research Personnel; Research Support; Research Training; Resource Sharing; Resources; Sampling; Services; Slice; Statistical Data Interpretation; Technology; Testing; Time; Tissues; Tracer; Training; Training and Education; Translating; Universities; Volatile Fatty Acids; Washington; advanced analytics; base; cancer care; cancer immunotherapy; cancer prevention; complex data; cost effective; design; education resources; effective therapy; experience; experimental study; improved; instrument; interest; knowledge base; lipidomics; liquid chromatography mass spectrometry; member; metabolic abnormality assessment; metabolomics; metabolomics resource; new technology; operation; programs; ranpirnase; response; small molecule; stable isotope; statistics; symposium; tool; tumor immunology

PROJECT SUMMARY: PROTEOMICS & METABOLOMICS SHARED RESOURCE (P&MSR) The Proteomics and Metabolomics Shared Resource (P&MSR) consists of the Fred Hutch (FH)-based Proteomics Facility and the University of Washington South Lake Union (UW-SLU) Metabolomics Facility. The P&MSR Proteomics Facility was formed in 2002 and has been under the direction of Dr. Phil Gafken since its inception. The P&MSR Metabolomics Facility was formed in 2013 and has been under the direction of Dr. Daniel Raftery. The mission of the P&MSR is (1) to provide high-quality, cost-effective, and reliable service in a timely manner for proteomics and metabolomics experiments; (2) to develop and implement new omics experiments, assays, and tools to uncover the molecular details influencing cancer biology; and (3) to support the development of translatable and clinical tests. The P&MSR operates and maintains chromatography, mass spectrometry, and nuclear magnetic resonance instruments and offers services such as experimental design and consultation, sample preparation and chromatography, protein or metabolite identification and quantification, protein modification characterization, targeted protein or metabolite quantification, metabolic flux analysis, and data analysis and informatics support. New technologies and services are continually being evaluated based on feedback from Consortium members. Over the next granting period, areas of interest that will be investigated include the analysis of major histocompatibility complex peptides to support cancer immunology and immunotherapy research. For metabolomics we will add to our over 20 current assays by expanding our capabilities to measure metabolic flux, provide the capability to investigate real-time metabolism studies using stable isotope tracers, and develop higher throughput global profiling assays to support research in cancer biology. With well-established operation policies, Consortium users have cost-effective, reliable, and immediate access to the P&MSR. The research supported by the P&MSR demonstrates that it is an important part of the critical Shared Resources infrastructure needed to support the Fred Hutch/University of Washington Cancer Consortium.

项目摘要：蛋白质组学和代谢组学共享资源（P＆MSR） 蛋白质组学和代谢组学共享资源（P＆MSR）由基于Fred Hutch（FH）组成 蛋白质组学设施和华盛顿大学南湖大学（UW-SLU）代谢组学设施。这 P＆MSR蛋白质组学设施成立于2002年，自从Phil Gafken博士的指导下 成立。 P＆MSR代谢组学设施成立于2013年，一直在博士的指导下。 丹尼尔·拉夫特（Daniel Raftery）。 P＆MSR的使命是（1）在A中提供高质量，具有成本效益和可靠的服务 及时的蛋白质组学和代谢组学实验的方式； （2）开发和实施新的OMIC 实验，测定和工具，以发现影响癌症生物学的分子细节； （3）支持 可翻译和临床测试的发展。 P＆MSR运行和维护色谱，质量 光谱法和核磁共振仪器，并提供实验设计等服务 以及咨询，样品制备和色谱，蛋白质或代谢物鉴定以及 定量，蛋白质修饰表征，靶向蛋白质或代谢物定量，代谢通量 分析，数据分析和信息学支持。新技术和服务一直是 根据财团成员的反馈进行评估。在下一个授予期内，感兴趣的领域 将研究包括对主要组织相容性复合肽的分析以支持癌症 免疫学和免疫疗法研究。对于代谢组学，我们将通过 扩大我们测量新陈代谢通量的能力，提供研究实时代谢的能力 使用稳定的同位素示踪剂进行研究，并开发较高的吞吐量全球分析测定法以支持研究 在癌症生物学中。有了完善的运营政策，财团用户具有成本效益，可靠和 立即访问P＆MSR。 P＆MSR支持的研究表明，这是一项重要的 支持弗雷德·哈奇/华盛顿大学所需的关键共享资源基础设施 癌症联盟。