Mommyomics and Babyomics - Effect of Gestational Diabetes on the Developing Infant Microbiome

Mommyomics 和 Babyomics - 妊娠糖尿病对发育中的婴儿微生物组的影响

• 批准号: 10312711
• 负责人: Kazune Catherine Pax
• 金额: $ 4.42万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10312711
• 关键词: Accounting; Adolescent obesity; Adult; Affect; American; Antibiotics; Anxiety; Attentional deficit; Bacteria; Behavioral; Bioinformatics; Biometry; Birth trauma; Cesarean section; Child; Child Health; Child Welfare; Chronic; Clinical Research; Cognitive; Complex; Correlation Studies; Cross-Sectional Studies; Data; Dental caries; Development; Diabetes Mellitus; Disease; Disease susceptibility; Emotional; Famines; Future; Genes; Genetic; Gestational Diabetes; Glucose Intolerance; Glycosylated hemoglobin A; Habits; Health; Health Promotion; Heritability; Human; Human Genome; Human Microbiome; Hyperactivity; Hypoglycemia; Infant; Infant Development; Intergenerational transfer; Intestines; Longitudinal Studies; Longitudinal prospective study; Maps; Maternal Health; Mental Depression; Mental Retardation; Metabolic; Metabolic Diseases; Metagenomics; Modeling; Mothers; Mouth Diseases; Mus; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Oral cavity; Oral health; Pattern; Periodontitis; Pre-Eclampsia; Predisposition; Pregnancy; Pregnant Women; Prevalence; Process; Prospective Studies; Research; Research Design; Research Personnel; Risk; Risk Assessment; Risk Factors; Route; Sampling; Smoking; Source; Stressful Event; Testing; Time; Vertical Disease Transmission; Whole-Genome Shotgun Sequencing; Woman; Women' s Health; adverse outcome; bacterial community; career; comparative; dynamic system; experience; feeding; gut microbiome; impaired glucose tolerance; insulin signaling; interest; malignant mouth neoplasm; maternal microbiome; microbiome; microbiome research; next generation sequencing; obesity in children; offspring; oral microbiome; oral pathogen; pathogen; prebiotics; pregnant; response; training opportunity; transmission process

Project Summary Maternal health is emerging as a critical long-term determinant of a child’s health. Therefore, promoting the health of women, especially pregnant women, promotes the well-being of children. The prevalence of gestational diabetes mellitus (GDM) has been steadily increasing, with current estimates at 13%. GDM is associated with adverse outcomes for both the mother and child. For example, there is an increased risk of pre-eclampsia and cesarean section for the mother and hypoglycemia, mental retardation, and birth trauma for the child. GDM has also been associated with an increased risk of future adolescent obesity for the child. Additionally, it has been found that GDM alters the maternal microbiomes, although the extent to which it impacts each ecological niche remains contested. Therefore, it is important to study the impact of GDM on the maternal microbiome to fully understand the impact of GDM at the metagenomic level. Furthermore, because there is intergenerational transfer of microbiomes and the microbiome is an important health determinant, studying the impact of GDM on the acquisition and development of the infant microbiome is critical to understanding risk for oral diseases. In the present proposal, it is hypothesized that the establishment of a stable, personalized infant oral microbiome is significantly influenced by maternal GDM and that the effects of GDM outweigh simple vertical transmission of the microbiome due to shared genetics between mothers and infants. This will be achieved by combining two independent yet integrated study designs with whole genome shotgun sequencing and strain-resolved comparative metagenomics. Both cross sectional and longitudinal study designs will be used to determine whether GDM impacts the oral microbiome, to determine the impact of maternal GDM on the developing infant oral microbiome, and to determine the relative attributions of vertical transmission and GDM to the developing infant oral microbiome. This will reveal the complexity and function of the microbiome at the gene level, stability over time and in response to perturbations, and to what extent the oral microbiome is a heritable feature, similar to the human genome. These findings will have major implications for disease and health including possible determinants of disease susceptibility, risk assessment and feasibility of strategies for prebiotics.

项目摘要 孕产妇的健康正成为对儿童健康的关键长期决定者。因此，促进 妇女的健康，尤其是孕妇的健康，促进了儿童的福祉。妊娠的患病率 糖尿病（GDM）一直在悄悄地增加，目前的估计为13％。 GDM与 母子和孩子的不利结果。例如，存在前启动前的风险和 母亲和低血糖的剖宫产部分，智力低下和儿童的出生创伤。 GDM有 还与儿童未来青少年肥胖的风险增加有关。另外，已经 发现GDM改变了母体微生物组，尽管它影响了每个生态位 仍然有争议。因此，重要的是研究GDM对母体微生物组的影响完全 了解GDM在宏基因组水平上的影响。此外，因为有代际 微生物组和微生物组的转移是重要的健康确定剂，研究了GDM对 婴儿微生物组的获取和开发对于理解口腔疾病风险至关重要。 在本提案中，假设建立一个稳定的个性化婴儿口服微生物组 受生物GDM的影响很大，GDM的影响超过简单的垂直传输 由于母亲和婴儿之间的遗传学共同遗传学而引起的微生物组。这将通过结合两个来实现 与整个基因组shot弹枪测序和应变分辨的独立但集成的研究设计 比较宏基因组学。横截面和纵向研究设计都将用于确定 GDM是否影响口服微生物组，以确定母体GDM对发育中的婴儿的影响 口服微生物组，并确定垂直传输和GDM的相对属性 婴儿口服微生物组。 这将揭示微生物组在基因水平上的复杂性和功能，随着时间的推移和稳定性 对扰动的响应，口腔微生物组在多大程度上是一个可遗传的特征，类似于人类 基因组。这些发现将对疾病和健康具有重大影响，包括可能的决定者 疾病易感性，风险评估和益生元策略的可行性。