Neural mechanisms of infant attachment

婴儿依恋的神经机制

基本信息

批准号：
10307129
负责人：
Elizabeth A.D. Hammock
金额：
$ 37.58万
依托单位：
FLORIDA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-01-01 至 2023-10-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10307129
关键词：
Acute Address Adult Affect Afferent Neurons Anatomy Autoradiography Behavior Behavioral Binding Birth Brain Caregivers Clinical Cre-LoxP Data Development Dose Face Female Fiber Foundations Frequencies Future Ganglia Goals Harvest Health Human Milk In Situ Hybridization Individual Differences Infant Infant Behavior Infant Development Knowledge Ligand Binding Link Liquid substance Long-Term Effects Mental Health Mental disorders Messenger RNA Milk Ejection Molecular Mothers Mus Neuroanatomy Neurons Newborn Infant Nociceptors OXT gene Oral Outcome Oxytocin Oxytocin Receptor Peptides Perinatal Peripheral Play Process Production Quality of life Reflex action Research Research Project Grants Risk Role Saliva Sensory Sensory Ganglia Sex Differences Signal Transduction Social Behavior Social Development Social Environment Source Stimulus Structure of trigeminal ganglion Subcellular Anatomy System Testing Trigeminal System Work behavior test brain behavior experimental study immunoreactivity improved insight intergenerational lifetime risk male neonatal mice neonatal period neonate neurobehavioral neuromechanism neuronal cell body novel postnatal preventive intervention receptor relating to nervous system response sensory mechanism social social contact social influence trafficking transmission process

项目摘要

Infant attachment to a caregiver is required for the typical development of social behavior and reduces the lifetime risk for mental illness. The underlying molecular and neural mechanisms of infant attachment to a caregiver are not well-characterized. Oxytocin (OXT) plays an important role in adult social behavior and has been hypothesized to play a role in infant attachment. This proposal will test a specific modulatory role for OXT acting at peripheral OXT receptors (OXTR) in infant attachment-related brain and behavior development. We have recently identified OXTR in the periphery of infant mice, including the face and oronasal cavity. Given the presence of OXT in maternal fluids such as saliva and breast milk, this suggests a simple hypothesis that maternal OXT could bind to infant peripheral OXTR to bias brain and behavioral development toward the social environment. In this proposal, we will determine the brain and behavioral effects of OXT acting upon peripheral OXTR in infant mice. First, we will perform anatomical studies to define the relevant circuitry from the periphery to the brain. Second, we will determine if these peripheral OXTR play a role in social orienting and contact behavior in neonates. Finally, we will determine if these peripheral OXTR influence the development of a key player in later social behavior- the infant’s own OXT production. Individual differences in the levels of OXT contribute to individual differences in social behavior and mental health. However, we know very little about how these individual differences in OXT are influenced by social contact in development or how social stimuli elicit OXT activity the socially naïve infant brain. This proposal will help address these knowledge gaps. Using infant mice, the long-term goals of this project are to understand 1) how social contact is transduced into the infant brain, 2) how the socially naïve neonate responds to exogenous OXT at the level of brain and behavior in the neonatal period, and 3) how social contact influences individual differences in OXT levels in the neonate. In addition, by performing these studies in male and female infant mice, we will ascertain potential sex differences in the acute response to social contact and OXT in the neonatal period. Further, because these experiments will clarify the roles of OXTR in the oronasal cavity, the findings may have broader applicability for understanding the mechanism of action of intranasal delivery of clinical doses of OXT. These experiments will determine the mechanisms of acute change in the neonate, with the potential for future experiments to determine the mechanisms of the long term effects of these changes.

婴儿对照料者的依恋是社会行为的典型发展所必需的，并减少了精神疾病的终身风险。婴儿附着在护理人员身上的基本分子和神经机制没有很好地表征。催产素（OXT）在成人社会行为中起着重要作用，并被认为在婴儿依恋中发挥作用。该提案将测试在婴儿依恋相关的大脑和行为发展中对OXT作用在外围OXT接收器（OXTR）上作用的特定调节作用。我们最近在婴儿小鼠的周围鉴定了OXTR，包括脸部和骨腔。鉴于诸如唾液和母乳之类的母体绒毛中存在OXT，这表明了一个简单的假设，即母体牛可以与婴儿外周的Oxtr结合，以使大脑和行为发展偏向社会环境。在此提案中，我们将确定OXTR作用于婴儿小鼠外周外OXTR的大脑和行为影响。首先，我们将进行解剖研究，以定义从周围到大脑的相关电路。其次，我们将确定这些外围Oxtr是否在新生儿的社会定向和接触行为中起作用。最后，我们将确定这些外围OXTR是否影响了后来社会行为的主要参与者的发展 - 婴儿自己的OXT生产。 oxt水平的个体差异会导致社会行为和心理健康的个体差异。但是，我们对OXT中的这些个体差异如何受到发展中的社会接触或社会刺激如何激发社会幼稚的婴儿大脑的影响很少了解。该建议将有助于解决这些知识差距。使用婴儿小鼠，该项目的长期目标是了解1）如何将社交接触转化为婴儿大脑，2）在新生儿时期，在大脑和行为水平上，社会幼稚的新生儿对外源性OXT的反应如何，以及3）社会接触如何影响新生儿OXT水平的个体差异。此外，通过在男性和雌性婴儿小鼠中进行这些研究，我们将确定在新生儿时期对社会接触和OXT的急性反应中的潜在性别差异。此外，由于这些实验将阐明OXTR在Oronasal腔中的作用，因此发现可能具有更广泛的适用性，以理解鼻内递送临床剂量OXT的作用机理。这些实验将确定新生儿急性变化的机制，并有可能使未来的实验确定这些变化的长期影响的机制。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Oxytocin via oxytocin receptor excites neurons in the endopiriform nucleus of juvenile mice.

DOI：
10.1038/s41598-022-15390-5
发表时间：
2022-07-06
期刊：
SCIENTIFIC REPORTS
影响因子：
4.6
作者：
Biggs, Lindsey M.;Hammock, Elizabeth A. D.
通讯作者：
Hammock, Elizabeth A. D.

Specificity of plasma oxytocin immunoassays: A comparison of commercial assays and sample preparation techniques using oxytocin knockout and wildtype mice.

DOI：
10.1016/j.psyneuen.2021.105368
发表时间：
2021-10
期刊：
Psychoneuroendocrinology
影响因子：
3.7
作者：
Gnanadesikan GE;Hammock EAD;Tecot SR;Carter CS;MacLean EL
通讯作者：
MacLean EL

Neurophysin I is an analytically robust surrogate biomarker for oxytocin.

Neurophyn I 是一种分析可靠的催产素替代生物标志物。

DOI：
10.1016/j.psyneuen.2023.106951
发表时间：
2024
期刊：
Psychoneuroendocrinology
影响因子：
3.7
作者：
MacLean,EvanL;Carranza,Elizabeth;Gnanadesikan,GitanjaliE;King,KatherineM;Allen,AliciaM;Linde-Krieger,LinneaB;Feldman,Ruth;White-Traut,RosemaryC;Hammock,ElizabethAD;Carter,CSue;Leng,Gareth;Tecot,StaceyR;Bell,AleecaF
通讯作者：
Bell,AleecaF