A Screen for Identifying Insomnia Genes.

识别失眠基因的屏幕。

基本信息

批准号：
10228112
负责人：
Alex C Keene
金额：
$ 1.45万
依托单位：
FLORIDA ATLANTIC UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-05-15 至 2021-08-06
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10228112
关键词：
Acute Address Affect Aggressive behavior Alleles Anatomy Animal Model Architecture Atlases Automobile Driving Behavioral Biological Models Brain Brain imaging Brain region CRISPR screen CRISPR/Cas technology Calcium Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Communities Development Disease Enzymes Evolution Exhibits Exploratory/Developmental Grant for Diagnostic Cancer Imaging Eye Fatigue Fishes Functional Imaging Gene Expression Gene Transfer Techniques Genes Genetic Genetic Models Head Health Hour Human Hyperphagia Image Impaired cognition Individual Individual Differences Investigation Measures Medicine Melatonin Metabolic dysfunction Methodology Mexican Modeling Mutagenesis Neuroanatomy Neurons Neuropeptides Neurophysiology - biologic function Obesity Periodicity Phenotype Physiology Play Population Preparation Process Protocols documentation Research Resources Rivers Role Scanning Sleep Sleep Deprivation Sleep Disorders Sleep disturbances Sleeplessness Stream Structure Surface System Transgenic Organisms Up-Regulation Variant Vertebrates base circadian comparative experimental study genetic manipulation genetic technology genetic variant high throughput screening hypocretin implementation facilitation in vivo insight mutant nervous system disorder neural circuit neuromechanism neuroregulation novel relating to nervous system response screening sleep regulation tool

项目摘要

Project Summary Evolutionary medicine leverages the power of naturally occurring phenotypic differences derived through adaptation to investigate genetic mechanisms underlying diseases, including neurological disorders. The Mexican cavefish exhibits a dramatic evolution of sleep loss compared to ancestral surface fish of the same species, providing a unique and powerful model to identify genetic factors regulating sleep. The proposed experiments will implement genetic technology in Mexican cavefish to identify novel regulators of naturally occurring insomnia-like phenotypes in cavefish. This proposal will also apply a newly developed neuroanatomical brain atlas and whole-brain calcium imaging to determine how novel sleep genes impact brain structure and function. These experiments will provide critical insight into the mechanistic basis of sleep regulation and identify naturally occurring genetic variants that contribute to sleep regulation. Further, the genetic tools and methodology established in these studies will provide valuable resources that can be applied to address questions beyond the scope in this proposal, including diseases such as eye-degeneration, obesity, hyperphagia and aggression that are commonly studied in this system.

项目概要进化医学利用自然发生的表型差异的力量适应研究疾病的遗传机制，包括神经系统疾病。这与祖先的表层鱼类相比，墨西哥洞穴鱼在睡眠不足方面表现出巨大的进化物种，提供了一个独特而强大的模型来识别调节睡眠的遗传因素。拟议的实验将在墨西哥洞穴鱼中实施基因技术，以确定新的自然调节剂洞穴鱼中出现类似失眠的表型。该提案还将应用新开发的神经解剖脑图谱和全脑钙成像以确定新的睡眠基因如何影响大脑的结构和功能。这些实验将为睡眠的机制基础提供重要的见解调节并识别有助于睡眠调节的自然发生的遗传变异。此外，这些研究中建立的遗传工具和方法将提供可应用的宝贵资源解决本提案范围之外的问题，包括眼睛退化、肥胖等疾病，该系统中经常研究的贪食症和攻击性。