Cell & Molecular Imaging Core

细胞

基本信息

批准号：
10226348
负责人：
Anna K Majewska
金额：
$ 18.5万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10226348
关键词：
Address Algorithms Animal Model Animals Biological Biological Process Biometry Brain Clinical Sciences Collaborations Communities Complex Analysis Computer software Computers Consultations Custom Data Data Analyses Data Collection Data Set Ensure Equipment Experimental Designs Facility Designs Faculty Human Image Image Analysis Imaging Device In Vitro Individual Infrastructure Institutes Intellectual and Developmental Disabilities Research Centers Label Laboratories Laboratory Research Methodology Methods Microscopy Modification Performance Phase Play Process Protocols documentation Reproducibility Research Research Personnel Research Project Grants Resolution Resources Role Sampling Scientist Signal Pathway Standardization System Technical Expertise Techniques Technology Time Tissues Training Translational Research Universities Viral Viral Vector base cellular imaging data acquisition data management data sharing design experience experimental study image processing imaging modality improved in vivo in vivo imaging instrumentation interest member molecular imaging programs spectrograph success theories tool

项目摘要

Project Summary The URMC IDDRC Cell and Molecular Imaging Core (CMI Core) provides IDDRC members with access to advanced imaging, analysis, and viral vector-based transduction methods, as well as corresponding faculty expertise, to support in vitro and in vivo study of phenomena at the subcellular, cellular, tissue, and whole animal scale. The capabilities provided are central to research conducted by IDDRC investigators who have been using the available techniques to study subcellular, cellular, and systems level processes in the developing brain of animal models and humans. The integration of these cutting edge methods with expert faculty guidance ensures and streamlines access to techniques and equipment that are fundamental to IDD research. State-of-the-art imaging equipment and software provides a spectrum of image acquisition and analysis approaches (confocal, multiphoton and super-resolution imaging) and our viral vector facility designs and produces custom viral approaches for both labeling and manipulating signaling pathways for in vitro and in vivo imaging and analysis. The CMI Core is supported by dedicated administrative and technical faculty and staff to provide customized, critical technical expertise in the design, implementation, and analysis of studies involving microscopy that span the interests of the IDDRC faculty. The CMI Core leverages existing expert faculty and staff and long-standing infrastructure (confocal, multiphoton, analysis), with a proven track record of usage by IDDRC investigators, with newer capabilities (super-resolution, viral vectors), which have been implemented based upon recognized needs. The result is a CMI Core that allows IDDRC investigators to design and implement meaningful experiments using cutting edge technologies and efficient analysis paradigms to address difficult and diverse biological problems.

项目摘要 URMC IDDRC单元格和分子成像核心（CMI核心）为IDDRC成员提供了访问高级成像，分析和基于病毒载体的转导方法以及相应的教师专业知识，以支持体外和体内研究现象的体内研究亚细胞，细胞，组织和整个动物尺度。提供的功能是 IDDRC调查人员进行的研究一直使用可用技术研究动物模型发展中的大脑中细胞，细胞和系统水平过程和人类。这些尖端方法与专家教师指导的集成确保并简化了对IDD研究至关重要的技术和设备的访问。最先进的成像设备和软件提供了一系列图像获取和分析方法（共聚焦，多光子和超分辨率成像）和我们的病毒载体设施设计并生产用于标签和操纵的定制病毒方法体外和体内成像和分析的信号通路。 CMI核心由专门的行政和技术教职员工以及提供定制的至关重要的技术涉及显微镜的研究，实施和分析方面的专业知识 IDDRC教师的利益。 CMI核心利用现有的专家教职员工以及长期存在的基础架构（共聚焦，多光子，分析），并具有可靠的使用记录由IDDRC调查人员，具有较新功能（超分辨率，病毒载体）的研究人员根据公认的需求实施。结果是允许IDDRC的CMI核心研究人员使用尖端技术设计和实施有意义的实验和有效的分析范例，以解决困难和多样化的生物学问题。