Gas Chromatography-Molecular Rotational Resonance Spectrometer

气相色谱-分子旋转共振光谱仪

基本信息

批准号：
10218225
负责人：
Justin Lindsay Neill
金额：
$ 60.4万
依托单位：
BRIGHTSPEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10218225
关键词：
3-Dimensional Adoption Amino Acids Amino Sugars Analytical Chemistry Applications Grants Area Award Basic Science Benchmarking Biological Biological Markers Biological Monitoring Complement Complex Mixtures Computer software Coupling Data Development Environmental Monitoring Environmental Pollutants Environmental Pollution Evaluation Gas Chromatography Generations Geometry Grant Health Heart Isomerism Laboratory Research Learning Libraries Liquid substance Mass Fragmentography Mass Spectrum Analysis Measurement Measures Methods Molecular Molecular Structure Molecular Weight Natural Product Drug Natural Products Paper Peer Review Performance Pharmaceutical Preparations Pharmacologic Substance Phase Protocols documentation Reference Standards Research Research Personnel Risk Rotation Sampling Series Shapes Small Business Innovation Research Grant Source Specificity Speed Structure System Techniques Technology Testing Tissues Toxin United States National Institutes of Health Water Work base biological systems commercialization design detector drug discovery enantiomer improved innovation insight instrument mass spectrometer metabolomics prototype screening small molecule

项目摘要

Gas Chromatography-Molecular Rotational Resonance Spectrometer NIH SBIR Direct to Phase II BrightSpec, Inc. Project Summary This project proposes the development of the first commercial gas chromatography-molecular rotational resonance (GC-MRR) spectrometer for analytical characterization of complex mixtures such as biological fluids and tissues, natural products, or environmental water sources. MRR is a spectroscopic technique for unambiguous structure determination and quantitation of small molecules, which has been used for decades in academic research laboratories but only recently commercialized by BrightSpec, driven by recent groundbreaking innovations. The key advantages of MRR motivating its incorporation into a gas chromatography hyphenated system are its selectivity to isomeric compounds, absolute quantitation capabilities, and identification of unknowns without reference standards. GC-MRR not only complements existing techniques (such as mass spectrometry and NMR), but also adds new insights to enhance discovery in numerous fields of research. A proof-of-concept prototype GC-MRR instrument has been used to demonstrate its capabilities to resolve complex mixtures, including co-eluting isotopologues and isotopomers. This preliminary work has de-risked this proposed Phase II project, where this prototype will be refined to develop a product with the sensitivity, speed, and ease-of-use necessary to facilitate its commercial adoption. This includes both the instrument hardware to quickly and precisely measure MRR spectra of analytes as they elute from the chromatographic column, and the software interface to allow users to easily set up measurements, visualize data, and interpret the results. The product will then be tested thoroughly on relevant samples in the market areas where GC-MRR is expected to have the greatest impact. Finally, following this extensive evaluation, the product design will be refined for commercial launch at or before the end of the grant period. The completed GC-MRR product holds the promise to enable breakthrough research in a number of high-impact health-related fields, including metabolomics, natural products drug discovery, and monitoring of environmental pollutants, among others.

气相色谱分子旋转共振光谱仪 NIH SBIR直接进入II期 Brightspec，Inc。项目摘要该项目提出了第一个商业气相色谱分子的开发旋转共振（GC-MRR）光谱仪用于复杂混合物的分析表征例如生物流体和组织，天然产物或环境水源。 MRR是一个光谱技术，用于明确结构的确定和定量小的光谱技术分子，该分子在学术研究实验室已有数十年，但直到最近才由Brightspec商业化，这是最近开创性创新的驱动。关键优势 MRR激励其将其纳入气相色谱连字符系统是其对异构化合物的选择性，绝对定量能力和识别没有参考标准的未知数。 GC-MRR不仅补充了现有技术（例如作为质谱和NMR），但也添加了新的见解，以增强许多发现研究领域。概念验证原型GC-MRR仪器已用于展示其功能解决复杂的混合物，包括共同洗复的同位素和同位素。这初步工作使这一拟议的II期项目降低了风险，该项目将完善该原型以促进其敏感性，速度和易用性来开发产品商业收养。这包括仪器硬件以快速而精确的测量分析物的MRR光谱从色谱柱中洗脱时，软件接口允许用户轻松设置测量，可视化数据并解释结果。这然后将对GC-MRR的市场区域中的相关样品进行彻底测试预计会产生最大的影响。最后，经过广泛的评估，该产品设计将在赠款期结束时或之前进行商业发布。完整的GC-MRR产品有望在数量中实现突破性研究与健康有关的领域，包括代谢组学，天然产品发现和监测环境污染物等。