Component for Institution # 264313

机构组件

基本信息

批准号：
10207563
负责人：
ELLEN M UNTERWALD
金额：
$ 24.29万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-30 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10207563
关键词：
AIDS/HIV problem Acquired Immunodeficiency Syndrome Anhedonia Animal Model Animals Anxiety Behavior Behavior assessment Behavioral Behavioral Model Brain Breeding Cognition Collaborations Communities Data Data Analyses Drug Regulations Equipment Ethanol Food Funding Generations Goals HIV-1 Human Resources Infection Institution Intravenous Knowledge Learning Locomotion Measurement Measures Memory Mental Depression Methods Modeling Mus Mutant Strains Mice National Institute of Drug Abuse Oral Pain Pathway interactions Pharmaceutical Preparations Procedures Rattus Relapse Research Research Design Research Personnel Research Project Grants Research Support Resources Rewards Rodent Model Saccharin Scientific Advances and Accomplishments Self Administration Self Stimulation Stress Stress Tests Substance abuse problem Sucrose Techniques Testing Training Transgenic Organisms Ultrasonics United States National Institutes of Health Universities Work addiction animal breeding anxiety-like behavior behavior test behavioral economics cognitive testing conditioned place preference design drug of abuse drug reinforcement emotional functioning experience experimental study humanized mouse in vivo in vivo evaluation innovation member mouse model mutant negative affect neurobiological mechanism novel therapeutics preference programs ranpirnase skills stereotypy vocalization ward

项目摘要

The Animal Core for Addiction Related Behaviors of the NIDA P30 Center at Temple University will support collaborative NIH-funded research projects using in vivo rodent models in order to advance scientific knowledge on drugs of abuse, addiction, pain and the intersection of drugs of abuse with HIV/AIDS. One aim of the Core is to provide the scientific expertise, personnel, equipment and other resources that are necessary to assess drug reinforcement, reward, reinstatement, and related endpoints of cognition and emotional function in rats and mice as needed to rigorously evaluate potential new therapeutic compounds and targets, as well as to elucidate neurobiological mechanisms underlying addiction. Another aim of the Core is to provide mutant rodent models to P30 collaborating investigators in order to enable study of specific molecules and pathways in vivo. Breeding animals in a central facility reduces the expense and promotes access of these valuable models to multiple researchers. Core personnel will work with investigators to design experiments including an emphasis on appropriate controls, data analysis, and result interpretation. If requested by collaborators, the Animal Core will provide training in behavioral methods, or Core staff will perform the tests independently. The Core offers the following approaches: intravenous and oral drug and ethanol self- administration, operant responding for food or sucrose, conditioned place preference and aversion, intracranial self-stimulation, learning and memory assessments, anxiety- and depression-like behavioral tests, use of stress models and other methods as needed. New innovative behavioral assessments and animal models are proposed in this renewal application in order to enhance the impact of the research supported by the Animal Core for Addiction Related Behaviors. These include the application of behavioral economic methods and intermittent access paradigms to drug self-administration studies, measurements of ultrasonic vocalization to assess positive and negative affect during behavioral testing, breeding of transgenic Cre rats, and support of humanized mouse models of HIV-1 infection. The Core serves as a national resource by providing the knowledge, skills and equipment necessary to enhance research programs and to promote extension of NIH-funded projects to include behavioral endpoints and animal models relevant to substance abuse research.

天普大学 NIDA P30 中心的成瘾相关行为动物核心将支持 NIH 资助的使用体内啮齿动物模型的合作研究项目，以推进关于滥用药物、成瘾、疼痛以及滥用药物与药物相互作用的科学知识艾滋病毒/艾滋病。核心的目标之一是提供科学专业知识、人员、设备和其他评估药物强化、奖励、恢复和相关的必要资源根据需要严格评估大鼠和小鼠的认知和情绪功能终点潜在的新治疗化合物和靶点，以及阐明神经生物学机制潜在的成瘾。 Core 的另一个目标是为 P30 合作提供突变啮齿动物模型研究人员以便能够研究体内特定分子和途径。饲养动物在中央设施降低了费用并促进这些有价值的模型获得多个研究人员。核心人员将与研究人员合作设计实验，包括重点适当的控制、数据分析和结果解释。如果合作者要求， Animal Core 将提供行为方法培训，或者 Core 工作人员将进行测试独立。核心提供以下方法：静脉注射和口服药物以及乙醇自我治疗管理，对食物或蔗糖的操作反应，条件性位置偏好和厌恶，颅内自我刺激、学习和记忆评估、焦虑和抑郁样行为测试、使用压力模型和其他需要的方法。新的创新行为评估和本次更新申请中提出了动物模型，以增强研究的影响力得到成瘾相关行为动物核心的支持。这些包括应用药物自我管理研究的行为经济学方法和间歇性获取范式，测量超声波发声以评估行为过程中的积极和消极影响测试、转基因 Cre 大鼠的培育以及 HIV-1 感染人源化小鼠模型的支持。核心作为国家资源，提供必要的知识、技能和设备加强研究计划并促进 NIH 资助项目的扩展，将行为学纳入其中与药物滥用研究相关的终点和动物模型。