Receptors and channels controlling sensation and behavior in Aedes aegypti

控制埃及伊蚊感觉和行为的受体和通道

• 批准号: 10207825
• 负责人: CRAIG MONTELL
• 金额: $ 51.35万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10207825
• 关键词: Address; Aedes; Affect; Auditory; Behavior; Behavioral Assay; Blood; Botanicals; Bypass; Carbon Dioxide; Cellular biology; Chemicals; Chemoreceptors; Communicable Diseases; Computer software; Consumption; Cues; Culicidae; Data; Dengue; Detection; Development; Devices; Disease; Disease Vectors; Drosophila genus; Effectiveness; Electrophysiology (science); Esthesia; Female; Female sterility; Genes; Genetic; Glean; Goals; Hearing; Human; Infrared Rays; Insect Control; Insect Repellents; Insecta; Knock-out; Lead; Light; Male Sterility; Molecular Genetics; Monitor; Mosquito-borne infectious disease; Mutate; Mutation; Neuromodulator Receptors; Neurons; Opsin; Partner in relationship; Plants; Population; Reproduction; Research; Rhodopsin; Role; Sensory; Sensory Receptors; Signal Transduction; Skin; Skin Temperature; Speech; Stimulus; TRP channel; TRPA channel; TRPV channel; Testing; Ursidae Family; Virus; Visual; Wing; Work; Yellow Fever; ZIKA; base; deaf; design; disease transmission; egg; experimental study; genetic approach; genetic element; genetic manipulation; improved; innovation; insight; male; mating behavior; multimodality; mutant; olfactory receptor; prevent; receptor; response; sensory input; sensory stimulus; sterile insect technique; success; tool; ward

Mosquitoes such as Aedes aegypti are disease vectors that rely on their keen senses to locate human hosts, and for finding conspecifics, so that they can mate. However, we have only a rudimentary understanding of the receptors that control these critical behaviors. The goal of the proposed research is to address this gap. To find human hosts, female Ae. aegypti integrate information from diverse stimuli, including CO2, visual cues, organic molecules, and skin temperature. We have discovered another cue. Aim 1 builds on our preliminary data that Ae. aegypti use infrared (IR) radiation as an additional host stimulus. We outline experiments to reveal the IR-sensing neurons, and the receptor that detects IR. To pursue this aim, we devised a highly effective behavioral assay for monitoring IR attraction, and a new molecular genetic approach to bypass complications in combining multiple genetic elements. Aim 2 takes advantage of a mutation that we recently created in a TRP channel, which renders males and females deaf. We will test the roles this channel and hearing in swarm formation, mating and in finding human hosts. Our proposed experiments will interrogate the iconoclastic idea that mosquitoes are attracted to human speech. This would be a particularly specific cue to help them recognize and zero in on people. In Aim 3, we propose a new strategy to overcome a major impediment limiting the efficacy of the sterile insect technique (SIT). SIT is a promising strategy to suppress Ae. Aegypti. It involves inundating a local population with sterile males, which render females sterile upon mating. However, an impediment to lasting suppression of mosquito populations is that wild-type males outcompete the sterile males. We propose two complementary approaches to significantly elevate male mating success. This work has the potential to transform SIT, and thereby effectively suppress Ae. aegypti. Aim 4 concerns another key sensory issue—identification of the receptors for repellents. We recently discovered that rhodopsins function as multi-modal sensory receptors, challenging 100 years of dogma that they detect only light. Here, we propose to test the hypothesis that an opsin in Ae. aegypti functions as a highly sensitive receptor for naturally-occurring insect repellents. If confirmed by our proposed experiments, this would demonstrate that opsins comprise a new, unsuspected class of olfactory receptor. To accomplish our goals, we will bring to bear an extensive repertoire of state-of-art approaches, some of which we have developed specifically to pursue this project. These include new molecular genetic tools, a suite of behavioral assays, original video tracking software, and several types of electrophysiological recordings. In summary, the unifying theme in this project is the identification of elusive receptors and mechanisms through which mosquitoes sense human hosts and conspecific mates. The insights gleaned from this work have exciting potential to lead to innovative strategies to control Ae. aegypti, and reduce insect-borne disease.

诸如埃及埃及埃及的蚊子是疾病媒介，它们依靠其敏锐的感觉来定位人类宿主， 并要查找考虑因素，以便他们可以交配。但是，我们只有对 控制这些关键行为的接收者。拟议的研究的目的是解决这一差距。找到 人类主持人，女性AE。来自潜水员刺激的埃及整合信息，包括二氧化碳，视觉提示，有机 分子和皮肤温度。我们发现了另一个提示。 AIM 1基于我们的初步数据 Ae。 Aegypti使用红外线（IR）辐射作为额外的宿主刺激。我们概述了揭示IR感应神经元的实验，以及检测IR的接收器。为了实现这一目标，我们设计了一种高效的行为 测定用于监测IR吸引力的测定，以及一种绕过并发症的新分子遗传方法 结合多个遗传元素。 AIM 2利用了我们最近在TRP中创建的突变 频道，使男性和女性聋。我们将测试这个频道的角色，并在群中听到 形成，交配和寻找人类寄主。我们提出的实验将询问Iconoclastic Idey 蚊子被人类的言论所吸引。这将是一个特别具体的提示来帮助他们 认可并为人零。在AIM 3中，我们提出了一种克服重大障碍的新策略 限制无菌绝缘技术的效率（SIT）。 SIT是抑制AE的有前途的策略。埃及。它 涉及淹没无菌男性的当地人口，这使女性交配时无菌。然而， 持续抑制蚊子种群的障碍是野生型雄性胜过无菌 男性。我们提出了两种完整的方法，以显着提高男性交配的成功。这项工作 有可能改变坐姿，从而有效抑制AE。埃及。目标4关注另一个钥匙 感官问题 - 确定驱虫剂的接收器。我们最近发现Rhodopsins功能 作为多模式的感觉受体，挑战他们仅检测光的100年教条。在这里，我们 提出测试AE中的Opsin的假设。埃及充当天然绝缘驱虫剂的高度敏感受体。如果我们提出的实验确认，这将证明Opsins 包括一种新的无规律的嗅觉受体。为了实现我们的目标，我们将带来 最先进的方法的广泛曲目，我们专门为此而开发了一些曲目 项目。其中包括新的分子遗传工具，一套行为测定套件，原始视频跟踪 软件和几种类型的电生理记录。总而言之，这个项目中的统一主题是 鉴定蚊子感觉到人类寄主的难以捉摸的接收器和机制 明显的伴侣。从这项工作中收集的见解具有令人兴奋的潜力，可以实现创新策略 控制AE。埃及，并减少抑制传播疾病。