The organizing principle of grouped olfactory receptor neurons

分组嗅觉受体神经元的组织原理

• 批准号: 10197096
• 负责人: Chih-Ying Su
• 金额: $ 23.7万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197096
• 关键词: Address; Afferent Neurons; Agreement; Behavior; Behavioral; Behavioral Assay; Biological; Biological Assay; Biology; Brain; Characteristics; Complex; Coupling; Courtship; Data; Drosophila genus; Exhibits; Female; Genetic; Goals; Grouping; Individual; Knowledge; Lateral; Light; Literature; Logic; Maps; Mediating; Modeling; Nature; Negative Valence; Neurons; Odors; Olfactory Pathways; Olfactory Receptor Neurons; Olfactory tract; Organism; Output; Oviposition; Peripheral; Positive Valence; Process; Publishing; Regulation; Reporting; Research; Rodent; Role; Sensory; Sensory Hair; Series; Signal Transduction; Smell Perception; Stereotyping; Stimulus; Surveys; System; Testing; Time; Work; base; egg; experimental study; extracellular; fly; hedonic; insight; male; optogenetics; preference; receptor; sensory system

PROJECT SUMMARY/ABSTRACT The goal of this project is to determine the organizing principle underlying olfactory receptor neuron (ORN) grouping in the antennal sensory hairs of Drosophila. For most sensory systems, neurons are arranged in an ordered manner to form topographic representations of stimuli from the external world. Understanding the organizing principle of such maps is critical for deciphering the nature of salient features that are extracted from parallel inputs through lateral inhibition. However in olfaction, whether and how neurons are organized into a sensory map remains largely unclear. In both rodents and flies, it is reported that neighboring glomeruli do not necessarily respond to similar odorants. In the absence of a chemotopic map, what then is the organizing principle for the olfactory system? To address this complex question, the research outlined here leverages the powerful genetic toolkit and tractable olfactory system of Drosophila. The general hypothesis— that ORNs are organized in the periphery based on the hedonic value of their detected odors to form a valence map—will be tested via systematic behavioral assays with optogenetic or thermogenetic activation of select receptor neurons. The proposal’s first aim is to determine the generality of the observation that in the same sensory hair, compartmentalized ORNs mediate opposing behavior—with attraction elicited by activation of the large-spike ORN(s) and aversion by the small-spike neighbor(s). The remaining aims propose to examine this organizing principle in sensory hairs implicated in regulating female egg-laying (Aim 2) or male courtship behavior (Aim 3). Successful execution of the proposal will likely bridge a longstanding knowledge gap in sensory biology by revealing, for the first time, olfactory maps within a complex organism.

项目摘要/摘要 该项目的目的是确定主要组织嗅觉受体神经元（ORN） 在大多数感觉系统的果蝇的触角中分组 从外部世界形成刺激的地形代表的有序方式。 这样的地图的组织原理对于解释提取的显着特征的性质至关重要 从平行输入到侧向入口。 在啮齿动物和苍蝇中，感觉到更大的感觉。 在没有趋化图的情况下，不一定对类似的气味做出反应。 组织嗅觉系统的原理？ 利用果蝇的强大遗传工具包和可拖动的橄榄型橄榄色系统。 该ORN是根据其检测到的气味的享乐值在外围组织的 地图 - 将通过精选的光遗传学或热激活的系统行为测定进行测试 受体神经元。 感觉头发，隔室化的ORN介导对面的对立行为 - 引起了吸引力 小尖峰ORN（S）和小尖峰邻居的厌恶旨在检查这一点。 在涉及女性卵子的感官头发中组织原理（AIM 2）或男性求爱 行为（目标3）。 感官生物学通过首次在复杂生物体中陶醉的嗅觉图。

项目成果

Mating increases Drosophila melanogaster females' choosiness by reducing olfactory sensitivity to a male pheromone.

• DOI: 10.1038/s41559-021-01482-4
• 发表时间: 2021-08
• 期刊: NATURE ECOLOGY & EVOLUTION
• 影响因子: 16.8
• 作者: Kohlmeier, Philip;Zhang, Ye;Gorter, Jenke A.;Su, Chih-Ying;Billeter, Jean-Christophe
• 通讯作者: Billeter, Jean-Christophe

Valence opponency in peripheral olfactory processing.

• DOI: 10.1073/pnas.2120134119
• 发表时间: 2022-02-01
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Wu ST;Chen JY;Martin V;Ng R;Zhang Y;Grover D;Greenspan RJ;Aljadeff J;Su CY
• 通讯作者: Su CY

Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster.

• DOI: 10.7554/elife.69896
• 发表时间: 2021-08-23
• 期刊: eLife
• 影响因子: 7.7
• 作者: Nava Gonzales C;McKaughan Q;Bushong EA;Cauwenberghs K;Ng R;Madany M;Ellisman MH;Su CY
• 通讯作者: Su CY