Effect of piezoelectric charges on oral microbiome modulation

压电电荷对口腔微生物组调节的影响

• 批准号: 10189941
• 负责人: Santiago Orrego
• 金额: $ 24.98万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-04 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10189941
• 关键词: Address; Affect; Ammonium; Anti-Bacterial Agents; Area; Bacteria; Barium; Biocompatible Materials; Biomass; Charge; Chemicals; Clinical; Clinical Services; Composite Dental Resin; Composite Resins; Dental; Dental Materials; Dental Restoration Failure; Dental caries; Dentin; Development; Economic Burden; Endodontics; Ensure; Environment; Excision; Experimental Designs; Failure; Filler; Formulation; Future; General Population; Generations; Goals; Grant; Growth; Health; Healthcare; In Vitro; Incidence; Ions; Life; Mastication; Measures; Metabolic; Microbial Biofilms; Microscopy; Modeling; Oral; Outcome; Pathogenicity; Patients; Pattern; Periodicity; Plant Resins; Property; Protocols documentation; Public Health; Quality of life; Recurrence; Research; Ribosomal RNA; Salivary; Sampling; Savings; Secondary Prevention; Sequence Analysis; Services; Silver; Streptococcus mutans; Surface; Technology; Time; Tissues; Tooth Diseases; Tooth Loss; Tooth structure; Translating; Underserved Population; Water; Work; Zinc Oxide; aged; biomaterial development; clinical practice; composite restoration; cost; dental resin; dysbiosis; improved; in vivo; innovation; mechanical properties; metatranscriptome; microbial; microbiome; microbiome composition; microbiome research; microorganism; models and simulation; nano; nanoparticle; oral biofilm; oral microbiome; pathogen; physical property; polymerization shrinkage; polymicrobial biofilm; premature; prevent; response; restoration; restorative dentistry; restorative material; seal; success; transcriptome sequencing

PROJECT SUMMARY Secondary caries is considered the leading cause of dental restorations failure. The economic burden of treating this dental condition is significant at a cost of over $5 billion each year in USA. Improving the seal of dental materials bonded to dental hard tissues is critical for the prevention of secondary caries, progression of dental disease and tooth loss. Removing bacteria at the bonded interface will prevent the (bio)chemical degradation of the bonded materials, and thus, increase the clinical service of restorations. A common limitation of current antibacterial technologies includes the short durability of the effects due to leaching and depletion of ions. Therefore, there is an immense need to develop a new generation of dental antibacterial materials to reduce and eliminate dental diseases at bonded interfaces (e.g. secondary caries). We recently developed a new composite with piezoelectric properties for antibacterial therapies by incorporating charged nano-fillers into dental resins. Our innovative approach significantly improves current technologies by using a single filler with long-lasting antibacterial effects. In this study, we propose to find the optimal amount of piezoelectric nano-filler to enable the antibacterial effect while retaining high mechanical and physical properties of the composite (aim 1). In addition, we will evaluate the oral microbiome-modulating effects of piezoelectric charges to understand the specific changes on the biofilm composition and identify the targeted species by piezoelectric charges (aim 2). We expect the development of the proposed technology to significantly impact the dental field by reducing the failure rate of composite restorations, in turn, reducing the costs associated with those failures, improving the health of patients, and preventing unnecessary loss of additional tooth structure.

项目摘要 次要龋齿被认为是牙齿修复失败的主要原因。经济负担 在美国，治疗这种牙齿状况的重大，每年的成本超过50亿美元。改善印章 与牙齿硬组织结合的牙科材料对于预防次龋齿至关重要 牙齿疾病和牙齿脱落。在粘合界面上去除细菌将防止（生物）化学 粘合材料的降解，从而增加修复体的临床服务。常见 当前抗菌技术的局限性包括由于浸出和 离子的耗竭。因此，迫切需要开发新一代的牙齿抗菌 减少和消除粘合界面处牙齿疾病的材料（例如次要龋齿）。我们最近 开发了一种具有压电特性的新复合材料，用于抗菌疗法 纳米填充物进入牙科树脂。我们的创新方法可通过使用大大改善当前技术 具有持久抗菌作用的单个填充物。在这项研究中，我们建议找到最佳的 压电纳米填充剂可实现抗菌作用，同时保持高机械和物理 复合材料的特性（AIM 1）。此外，我们将评估口服微生物组调节作用 压电电荷以了解生物膜组成的特定变化并确定目标 通过压电指控物种（AIM 2）。我们希望提出的技术的发展能够 通过降低复合修复体的失败率，从而显着影响牙齿场 与这些失败相关的成本，改善患者的健康状况，并防止不必要的损失 附加的牙齿结构。