ShEEP Request For A Pre-Clinical In-Vivo X-Ray Micro Computed-Tomography Scanner

ShEEP 请求临床前体内 X 射线微型计算机断层扫描仪

• 批准号: 10178581
• 负责人: GEORGE R. BECK
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10178581
• 关键词: 3-Dimensional; Anesthesia procedures; Animals; Award; Biocompatible Materials; Biological; Bone Density; Bone Diseases; Bone Regeneration; Bone Tissue; Bone structure; Collaborations; Collection; Computed Tomography Scanners; Computer Systems; Computer software; Contrast Media; Cost Savings; Custom; Data; Data Storage and Retrieval; Databases; Degenerative polyarthritis; Development; Disease; Doctor of Philosophy; Dose; Dual-Energy X-Ray Absorptiometry; Equipment; Evaluation; Floor; Fracture; Frequencies; Funding; Generations; Hand; Health; Heart; Heating; Homeostasis; Hormones; Image; In Vitro; Joints; Laboratory Animals; Longitudinal Studies; Low Dose Radiation; Measurement; Measures; Medical; Memory; Methodology; Methods; Monitor; Mus; Musculoskeletal; Names; Noise; Operative Surgical Procedures; Oryctolagus cuniculus; Osteoporotic; Pennsylvania; Productivity; Protocols documentation; Quality Control; Regenerative research; Research; Research Personnel; Resolution; Rodent; Rodent Model; Roentgen Rays; Sample Size; Sampling; Savings; Scanning; Schedule; Secure; Sheep; Skeleton; Software Tools; Specimen; Speed; Structure; Switzerland; System; Techniques; Technology; Temperature; Therapeutic Agents; Three-dimensional analysis; Time; Tissues; Touch sensation; Training; Transgenic Animals; Transgenic Mice; Tube; Vertebral column; Veterans; Visualization; Work; animal colony; animal facility; archive data; bone; bone quality; cortical bone; cytokine; data archive; data management; density; detector; drug development; experience; high resolution imaging; improved; in vivo; indexing; instrument; microCT; mouse model; operation; pre-clinical; programs; reconstruction; recruit; repaired; respiratory; response; substantia spongiosa; tissue regeneration; tissue repair; tool

Abstract We are requesting funds for the purchase of a state-of-the-art in vivo micro Computed Tomography (microCT) scanner for non-invasive, nondestructive, volumetric imaging and analysis of the skeleton from live animals ranging in size from mice to rabbits. The vivaCT80 is manufactured by Scanco Medical AG (Brutisellen, Switzerland) and distributed by Scanco USA, Inc. (King of Prussia, Pennsylvania, USA). The system is capable of acquiring and analyzing three-dimensional images of the skeleton and vasculature of live animals with a voxel size as small as 5µm while maintaining a relatively low radiation dose. The combination of low dose radiation, high speed, and a wide-ranging resolution and field of view in the vivaCT80 are made possible by an 8192 pixel wide detector. The vivaCT80 accommodates a wider range of animal/specimen sizes (90mm bore size with an 80mm field of view) than most competing high-resolution instruments. The x-ray tube is capable of peak energies from 30 kVp to 70 kVp which improves image quality (contrast-to-noise ratio, CNR) and reduces scan time and dose for both high- and low-density specimens The scanner includes an integrated touch display, heating system, animal temperature monitoring, observation camera, respiratory gating,heart beat frequency monitoring, and ports for anesthesia and contrast media, and all software needed for standard and customized volumetric and structural analyses. This instrument will be utilized by five major users, all VA funded with laboratories and animal colonies at the Atlanta VAMC; Drs. George R Beck Jr. (PI) (PhD), Neale Weitzmann (PhD), Hicham Drissi (PhD), Nick Willet (PhD), and Steven Presciutti (MD). In addition to 4 VA merits and 1 VA CDA award, these investigators also are funded by 3 R01s, 3 R21s, DOD, Score, and U54 projects, among others, all focused on musculoskeletal research. The research programs range in focus from diseases of bone homeostasis (i.e. osteoporotic disease), fracture repair, osteoarthritis, biomaterials for bone regeneration and tissue repair, and drug development for improved spine fusion. The equipment will be located in the animal facility on the 4th floor of Building A at the Atlanta VAMC (Decatur GA) where all five major users have animal colonies. The in vivo microCT will signficantly facilitate each of these projects focused on pre-clinical rodent models of bone disease and repair and be a great assest to the rapidly expanding Musculoskeletal group at the Atlanta VAMC. The advantages of having an in vivo microCT are several. 1) All five major users utilize transgenic mouse models It would make possible to expand the ongoing and planned studies with in vivo data about bone structure, a capability which is currently not on hand. 2) The greater sensitivity and precision of in vivo microCT over other methods would results in increased group separation and thus increased ability to interpret the data in a conclusive manner. 3) In many cases it will be possible to decrease the sample size and duration of the studies with considerable financial savings. 4) We would be able to conduct our studies at a much faster pace increasing productivity. 5) The increased capability of in vivo microCT would provide additional opportunity for collaborations and recruiting, training of new investigators and opening new fields of research.

抽象的 我们要求资金购买最先进的体内微型计算机断层扫描 （Microct）用于非侵入性，无损，体积成像的扫描仪和现场骨架的分析 动物的大小从小鼠到兔子。 Vivact80由Scanco Medical AG制造 （瑞士布鲁蒂斯伦），并由美国Scanco USA，Inc。（美国宾夕法尼亚州普鲁士国王）发行。这 系统能够获取和分析现场骨骼和脉管系统的三维图像 体素尺寸小至5µm的动物，同时保持相对较低的辐射剂量。结合 低剂量辐射，高速辐射以及Vivact80中的广泛分辨率和视野 由8192像素宽检测器可能。 Vivact80可容纳各种动物/标本大小 （比大多数竞争的高分辨率仪器比90mm的孔尺寸，具有80mm的视野）。 X射线管 能够从30 kVp到70 kVp的峰值能量，从而提高图像质量（对比度比率，CNR） 并减少了高密度和低密度标本的扫描时间和剂量，扫描仪包括一个集成的 触摸显示器，加热系统，动物温度监测，观察摄像头，呼吸门控，心脏 击败频率监测，以及用于麻醉和对比介质的端口，以及标准所需的所有软件 以及定制的体积和结构分析。 该工具将由五个主要用户使用，所有弗吉尼亚州都由实验室和动物殖民地资助 亚特兰大VAMC；博士。乔治·R·贝克（PI）（PHD），Neale Weitzmann（博士），Hicham Drissi（博士），尼克 Willet（博士）和Steven Presciutti（MD）。除了4个VA优点和1个VA CDA奖，这些调查人员还 还由3 R01，3 R21，DOD，SCORE和U54项目资助，等等 肌肉骨骼研究。研究计划的重点来自骨体内稳态疾病（即 骨质疏松性疾病），骨折修复，骨关节炎，用于骨再生和组织修复的生物材料以及 用于改善脊柱融合的药物开发。设备将位于四楼的动物设施中 在亚特兰大VAMC（Decatur GA）建造A，所有五个主要用户都有动物殖民地。 体内Microct将显着促进这些项目都集中在临床前啮齿动物模型上 骨骼疾病和修复，是亚特兰大快速扩张的肌肉骨骼群的重要屁股 vamc。具有体内微观的优势是几个。 1）所有五个主要用户都利用转基因 鼠标模型可以通过有关骨骼的体内数据扩展正在进行的研究和计划研究 结构，目前尚未在场的功能。 2）体内微观的灵敏度和精度更高 超过其他方法将导致小组分离增加，从而提高解释数据的能力 以结论性的方式。 3）在许多情况下，可以减少样本量和持续时间 具有可观的财务节省的研究。 4）我们能够以更快的速度进行研究 提高生产率。 5）体内Microct的功能提高将为额外的机会提供 合作和招聘，新研究人员的培训以及开放新的研究领域。