ShEEP Request For A Pre-Clinical In-Vivo X-Ray Micro Computed-Tomography Scanner

ShEEP 请求临床前体内 X 射线微型计算机断层扫描仪

• 批准号: 10178581
• 负责人: GEORGE R. BECK
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10178581
• 关键词: 3-Dimensional; Anesthesia procedures; Animals; Award; Biocompatible Materials; Biological; Bone Density; Bone Diseases; Bone Regeneration; Bone Tissue; Bone structure; Collaborations; Collection; Computed Tomography Scanners; Computer Systems; Computer software; Contrast Media; Cost Savings; Custom; Data; Data Storage and Retrieval; Databases; Degenerative polyarthritis; Development; Disease; Doctor of Philosophy; Dose; Dual-Energy X-Ray Absorptiometry; Equipment; Evaluation; Floor; Fracture; Frequencies; Funding; Generations; Hand; Health; Heart; Heating; Homeostasis; Hormones; Image; In Vitro; Joints; Laboratory Animals; Longitudinal Studies; Low Dose Radiation; Measurement; Measures; Medical; Memory; Methodology; Methods; Monitor; Mus; Musculoskeletal; Names; Noise; Operative Surgical Procedures; Oryctolagus cuniculus; Osteoporotic; Pennsylvania; Productivity; Protocols documentation; Quality Control; Regenerative research; Research; Research Personnel; Resolution; Rodent; Rodent Model; Roentgen Rays; Sample Size; Sampling; Savings; Scanning; Schedule; Secure; Sheep; Skeleton; Software Tools; Specimen; Speed; Structure; Switzerland; System; Techniques; Technology; Temperature; Therapeutic Agents; Three-dimensional analysis; Time; Tissues; Touch sensation; Training; Transgenic Animals; Transgenic Mice; Tube; Vertebral column; Veterans; Visualization; Work; animal colony; animal facility; archive data; bone; bone quality; cortical bone; cytokine; data archive; data management; density; detector; drug development; experience; high resolution imaging; improved; in vivo; indexing; instrument; microCT; mouse model; operation; pre-clinical; programs; reconstruction; recruit; repaired; respiratory; response; substantia spongiosa; tissue regeneration; tissue repair; tool

Abstract We are requesting funds for the purchase of a state-of-the-art in vivo micro Computed Tomography (microCT) scanner for non-invasive, nondestructive, volumetric imaging and analysis of the skeleton from live animals ranging in size from mice to rabbits. The vivaCT80 is manufactured by Scanco Medical AG (Brutisellen, Switzerland) and distributed by Scanco USA, Inc. (King of Prussia, Pennsylvania, USA). The system is capable of acquiring and analyzing three-dimensional images of the skeleton and vasculature of live animals with a voxel size as small as 5µm while maintaining a relatively low radiation dose. The combination of low dose radiation, high speed, and a wide-ranging resolution and field of view in the vivaCT80 are made possible by an 8192 pixel wide detector. The vivaCT80 accommodates a wider range of animal/specimen sizes (90mm bore size with an 80mm field of view) than most competing high-resolution instruments. The x-ray tube is capable of peak energies from 30 kVp to 70 kVp which improves image quality (contrast-to-noise ratio, CNR) and reduces scan time and dose for both high- and low-density specimens The scanner includes an integrated touch display, heating system, animal temperature monitoring, observation camera, respiratory gating,heart beat frequency monitoring, and ports for anesthesia and contrast media, and all software needed for standard and customized volumetric and structural analyses. This instrument will be utilized by five major users, all VA funded with laboratories and animal colonies at the Atlanta VAMC; Drs. George R Beck Jr. (PI) (PhD), Neale Weitzmann (PhD), Hicham Drissi (PhD), Nick Willet (PhD), and Steven Presciutti (MD). In addition to 4 VA merits and 1 VA CDA award, these investigators also are funded by 3 R01s, 3 R21s, DOD, Score, and U54 projects, among others, all focused on musculoskeletal research. The research programs range in focus from diseases of bone homeostasis (i.e. osteoporotic disease), fracture repair, osteoarthritis, biomaterials for bone regeneration and tissue repair, and drug development for improved spine fusion. The equipment will be located in the animal facility on the 4th floor of Building A at the Atlanta VAMC (Decatur GA) where all five major users have animal colonies. The in vivo microCT will signficantly facilitate each of these projects focused on pre-clinical rodent models of bone disease and repair and be a great assest to the rapidly expanding Musculoskeletal group at the Atlanta VAMC. The advantages of having an in vivo microCT are several. 1) All five major users utilize transgenic mouse models It would make possible to expand the ongoing and planned studies with in vivo data about bone structure, a capability which is currently not on hand. 2) The greater sensitivity and precision of in vivo microCT over other methods would results in increased group separation and thus increased ability to interpret the data in a conclusive manner. 3) In many cases it will be possible to decrease the sample size and duration of the studies with considerable financial savings. 4) We would be able to conduct our studies at a much faster pace increasing productivity. 5) The increased capability of in vivo microCT would provide additional opportunity for collaborations and recruiting, training of new investigators and opening new fields of research.

抽象的 我们正在申请资金购买最先进的体内微型计算机断层扫描 (microCT) 扫描仪，用于对活体骨骼进行非侵入性、非破坏性体积成像和分析 vivaCT80 由 Scanco Medical AG 制造。 （瑞士布鲁蒂塞伦）并由 Scanco USA, Inc.（美国宾夕法尼亚州普鲁士国王）分销。 系统能够采集和分析活体骨骼和脉管系统的三维图像 动物的体素尺寸小至 5μm，同时保持相对较低的辐射剂量。 vivaCT80 具有低剂量辐射、高速以及宽范围的分辨率和视场 vivaCT80 可容纳更广泛的动物/样本尺寸。 （孔径为 90 毫米，视场为 80 毫米）优于大多数竞争性高分辨率 X 射线管。 能够提供 30 kVp 至 70 kVp 的峰值能量，从而提高图像质量（对比度噪声比，CNR） 扫描减少了高密度和低密度样本的时间和剂量。扫描仪包括一个集成的 触摸显示屏、加热系统、动物体温监测、观察摄像头、呼吸门控、心脏 拍频监测、麻醉和造影剂端口以及标准所需的所有软件 以及定制的体积和结构分析。 该仪器将由五个主要用户使用，所有用户均由 VA 资助，实验室和动物群体位于 亚特兰大 VAMC 博士（PI）（博士）、Neale Weitzmann（博士）、Hicham Drissi（博士）、Nick Willet（博士）和 Steven Presciutti（医学博士）除了 4 项 VA 功绩和 1 项 VA CDA 奖外，这些研究人员还获得了 VA 荣誉奖和 VA CDA 奖。 也由 3 R01、3 R21、DOD、Score 和 U54 项目等资助，所有这些项目都专注于 研究项目的重点是骨稳态疾病（即骨稳态疾病）。 骨质疏松症）、骨折修复、骨关节炎、用于骨再生和组织修复的生物材料，以及 用于改善脊柱融合的药物开发该设备将位于四楼的动物设施中。 位于亚特兰大 VAMC（佐治亚州迪凯特）的 A 栋大楼，所有五个主要用户都有动物栖息地。 体内 microCT 将极大地促进这些专注于临床前啮齿动物模型的项目 骨疾病和修复，并成为亚特兰大快速扩张的肌肉骨骼小组的重要帮助 VAMC 拥有体内 microCT 的优点有很多： 1) 所有五个主要用户都使用转基因技术。 小鼠模型 这将使利用骨的体内数据扩大正在进行和计划的研究成为可能 结构，目前尚不具备这种能力 2) 体内 microCT 具有更高的灵敏度和精度。 与其他方法相比，会导致组分离增加，从而提高解释数据的能力 3) 在许多情况下，可以减少样本量和持续时间。 4）我们将能够以更快的速度进行研究。 5) 体内 microCT 能力的增强将为以下方面提供更多机会。 合作和招募、培训新研究人员以及开辟新的研究领域。