ShEEP Request for a SpectraMax M Series Multi-Mode Microplate Reader

ShEEP 请求 SpectraMax M 系列多功能酶标仪

• 批准号: 10177680
• 负责人: Carol A. Casey
• 金额: --
• 依托单位: OMAHA VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177680
• 关键词: 10 year old; Alcoholic Liver Diseases; Award; Biological Assay; Chronic lung disease; Collaborations; Computer software; Computers; DNA analysis; Dependence; Detection; Devices; Diabetes Mellitus; Disease model; Doctor of Philosophy; Equipment; Fluorescence; Fluorescence Polarization; Funding; Goals; Health; Legal patent; Liver diseases; Lung diseases; Measures; Medical center; Minor; Mission; Modeling; Nebraska; Optics; Organ; Performance; Process; Program Evaluation; Property; Protein Analysis; Reader; Reading; Recording of previous events; Request for Applications; Research; Research Infrastructure; Research Personnel; Resource Sharing; Resources; Sampling; Scanning; Scientist; Series; Services; Ships; Supervision; System; Temperature; Therapeutic Intervention; Time; Training; Universities; Veterans; career; cost; daily functioning; data management; enzyme activity; instrument; luminescence; non-alcoholic fatty liver disease; novel; pre-clinical; repaired; sensor; sensor technology

In this application, we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a Multi-Mode Microplate Reader. This instrument will be a shared resource to be housed in the Research Service of the Omaha VA Medical Center. Specifically, we seek to obtain a Spectra Max M Series Multi-Mode Microplate Reader. The SpectraMax M series allows for both cuvette and microplate reading and detection by UV, visible, fluorescence and luminescence. This important piece of equipment will provide state-of-the art capabilities not currently available on our VAMC campus. It will replace our current microplate reader, which is 10 years old and needs extensive and expensive repairs. With this new instrument, there is no need to utilize expensive filters to optimize detection levels and background. The optical systems use two scanning monochromators, so that the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. Additionally, the Spectra Max M Series offers the capability of measuring the depth (optical pathlength) of samples with no temperature dependency, using the patented PathCheck® Sensor technology. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. This eliminates the need for standard curves, and for compounds with known absorbance properties, enabling users to calculate concentrations directly from absorbance. The microplate reader will be extensively used by our investigators in the Research Service of the Omaha VAMC. The instrument will enable new capabilities for researchers at the Omaha VAMC and our affiliates at the University of Nebraska Medical Center (UNMC). Acquisition of the Spectramax will help us create new capabilities not currently available at this Medical Center. SpectraMax M Series will help to accelerate our research. Specifically, we are examining Veteran-relevant, preclinical disease models, including liver disease, diabetes and pulmonary disease in hopes of advancing the therapeutic interventions that will support the VA mission.

在此应用程序中，我们要求从ORD共享设备评估计划（Sheep）中购买资金 多模式微型读取器。该工具将是研究服务中的共享资源 奥马哈弗吉尼亚州医疗中心。具体而言，我们寻求获得Spectra Max M系列多模式微孔板 读者。 Spectramax M系列允许使用紫外线，可见的紫外线读取和检测，可见， 荧光和发光。这项重要的设备将提供最先进的功能而不是 目前在我们的VAMC校园中可用。它将取代我们目前的微板读取器，该读取器已有10年的历史 需要大量昂贵的维修。有了这种新仪器，无需使用昂贵的过滤器来 优化检测水平和背景。光学系统使用两个扫描单色器，因此 用户可以确定最佳兴奋和排放设置，从而导致测定性能类似 专用的单模阅读器。此外，Spectra Max M系列提供了测量的能力 使用专利的Patheck®传感器，没有温度依赖性的样品的深度（光路长） 技术。使用SoftMax Pro软件，Patheck传感器会自动使井吸光度归一化。 这消除了对具有已知吸收特性的化合物的需求 用户可以直接从吸收中计算浓度。微板读取器将被广泛使用 我们的研究人员在奥马哈VAMC的研究服务中。该仪器将启用新功能 奥马哈VAMC的研究人员和我们的内布拉斯加州医学中心（UNMC）的分支机构。 收购Spectramax将帮助我们创建当前在此医疗中心无法使用的新功能。 Spectramax M系列将有助于加速我们的研究。具体而言，我们正在研究与资深人士相关的 临床前疾病模型，包括肝病，糖尿病和肺部疾病，希望推进 将支持VA任务的治疗干预措施。