ShEEP Request for KEYENCE BZ-X800 All-in-One Fluorescence Microscope

SheEEP 请求购买 KEYENCE BZ-X800 一体式荧光显微镜

• 批准号: 10177103
• 负责人: PATTY J LEE
• 金额: --
• 依托单位: DURHAM VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177103
• 关键词: 3-Dimensional; Adhesions; Adult Respiratory Distress Syndrome; Affect; Air Pollution; Airway Disease; Americas; Antioxidants; Area; Autoantigens; Bacterial Infections; Binding; Budgets; COVID-19; Carbon Dioxide; Cardiovascular Diseases; Cell Count; Cell Survival; Cell model; Cells; Chicago; Chronic; Chronic Obstructive Airway Disease; Collaborations; Color; Complication; Computer software; Contracts; Cultured Cells; Cyclin-Dependent Kinase Inhibitor 2A; DNA Repair; Data; Data Analyses; Department of Defense; Development; Diabetes Mellitus; Diabetic Nephropathy; Disease; Endothelial Cells; Endothelium; Fibrosis; Fostering; Funding; Goals; Grant; Hamsters; Heating; Histologic; Histones; Hour; Hybrid Cells; Image; Incidence; Inflammation; Injury; Laboratories; Lead; Ligands; Light; Lighting; Lung; Maintenance; Manufacturer Name; Metals; Methods; Microscope; Minor; Modeling; Molecular; Monitor; Names; Oils; Organ; Phase; Pneumonia; Pulmonary Emphysema; RNA; Reproducibility; Research; Research Personnel; Resolution; SKIL gene; Safety; Sampling; Scientist; Sheep; Ships; Signal Pathway; Signal Transduction; Site; Slice; Smooth Muscle Myocytes; Source; Streptococcus pneumoniae; Structure; Substance abuse problem; System; Systemic Lupus Erythematosus; Temperature; Testing; Texas red; Time; Tissues; Training; United States National Institutes of Health; Vascular Diseases; Virus Diseases; aptamer; cell growth; comorbidity; contrast imaging; cyanine dye 5; data acquisition; design; digital; exposure to cigarette smoke; extracellular; fluorescence imaging; fluorescence microscope; imaging software; instrument; interest; lens; live cell imaging; military veteran; mouse model; podocyte; reconstruction; repaired; routine imaging; senescence; tool; ultraviolet

Project Summary / Abstract The Keyence BZ-X00 is a multi-functional and versatile microcopy platform for cells and tissues. It incorporates 6 objective lenses with magnification power from 2X up to 100X. It can be used to image with white light for histological examination of whole tissue slices and cells in culture, and by using filters for fluorescence imaging across the color spectrum. The BZ-X800 configuration in this proposal includes an integrated heating chamber with CO2 for extended live-cell imaging, facilitated by the included automatic focal plane correction software. It also incorporates an optional grid system for structured illumination that can be inserted before the light hits the sample to remove out of focus light and provide images with the resolution of confocal microscopes. Optional data analysis packages are included for automated stitching of large images, hybrid cell counting and 3D- reconstructions of cells and tissues. It is designed from the ground up as a stand-alone system with an enclosure that replaces the need for a dark room and with software controls that minimize the possibility of user errors leading to hardware malfunctions. • Lee: Current VA Merit, 5I01 BX003265-05, examining mechanisms of MIF-CD74 signaling in protecting against emphysema through activation of specific cell survival, antioxidant, and DNA repair molecules and the suppression of senescence protein p16. • Lee: Current DoD Grant, PR150809, is testing MIF augmentation against cigarette smoke exposure-related COPD and its subsequent complication, Streptococcus pneumoniae pneumonia • Spurney: Pending VA Merit Renewal: I01 BX002984-05 examines the molecular mechanisms that contribute to injury of glomerular podocytes in diabetic kidney disease • Spurney: Department of Defense grant (PR180545) and an NIH training grant (T32 DK007731) and is a co- investigator on an active R01 grant (R01-DK098135). • Miller: Current VA Merit, I01 BX001729, is examining molecular mechanisms by which chronic inflammation accelerates vascular disease. Studies are performed in cultured smooth muscle cells and a murine model of carotid injury. • Miller: Pending VA Merit, I01 BX005308, is a pre-IND enabling study to examine the safety of Synthetic RNA ligands for the treatment of hyperproliferative vascular disease. • Miller: I01 BX005088 is a four-year Merit that will identify RNA aptamers that selectively bind to inflamed endothelial cells and another aptamer to inhibit smooth muscle cell growth. Studies are performed in cultured cells and murine models of vascular disease. • Miller: I01 BX005450 is a two-year Merit that proposes to determine whether inactivation of extracellular histones by a previously described synthetic RNA ligand will protect from the development of acute respiratory distress syndrome (ARDS) in COVID-19. These studies are performed in hamster models of COVID-19. • Pisetsky: VA Merit, 5I01BX003772-02, Cellular Sources of Self Antigen in SLE • McMahon: VA Merit, VA BX-003478-01. SNO transport regulates endothelial adhesion of RBCs. The goal is to determine the mechanism by which RBCs export and endothelial cells import SNOs, forming a signaling pathway. • Tighe: The laboratory is interested in mechanisms by which air pollution alters lung structure and function. • Murdoch: Our studies relate to the impact of chronic inflammation on comorbidities and end organ disease, including airway disease and substance abuse-driven inflammation.

项目概要/摘要 Keyence BZ-X00 是一款用于细胞和组织的多功能多功能显微复制平台。 6 个物镜，放大倍数从 2 倍到 100 倍，可用于白光成像。 对整个组织切片和培养细胞进行组织学检查，并使用滤光片进行荧光成像 该提案中的 BZ-X800 配置包括一个集成加热室。 在随附的自动焦平面校正软件的帮助下，使用 CO2 进行扩展活细胞成像。 还包含一个可选的网格系统，可在光线照射到物体之前进行结构化照明 样品以消除失焦光并提供具有共焦显微镜分辨率的图像（可选）。 包含数据分析包，用于自动拼接大图像、混合细胞计数和 3D- 它是从头开始设计的，是一个带有外壳的独立系统。 取代了对暗室的需求，并使用软件控制来最大限度地减少用户错误的可能性 导致硬件故障。 • Lee：当前 VA 优点，5I01 BX003265-05，检查 MIF-CD74 信号传导在保护中的机制 通过激活特定的细胞存活、抗氧化剂和 DNA 修复分子来对抗肺气肿 衰老蛋白p16的抑制。 • Lee：目前的国防部拨款，PR150809，正在测试 MIF 增强对香烟烟雾暴露相关的影响 慢性阻塞性肺病及其后续并发症，肺炎链球菌肺炎 • Spurney：等待 VA 优异更新：I01 BX002984-05 检查有助于 糖尿病肾病中肾小球足细胞损伤的影响 • Spurney：国防部拨款 (PR180545) 和 NIH 培训拨款 (T32 DK007731)，并且是 活跃 R01 资助 (R01-DK098135) 的研究员。 • Miller：当前 VA 优异奖，I01 BX001729，正在研究慢性炎症的分子机制 加速血管疾病的研究是在培养的平滑肌细胞和小鼠模型中进行的。 颈动脉损伤。 • Miller：待定 VA 优异奖，I01 BX005308，是一项 IND 前研究，旨在检查合成 RNA 的安全性 用于治疗过度增殖性血管疾病的配体。 • Miller：I01 BX005088 是一项为期四年的优异奖，它将识别选择性结合炎症的 RNA 适体 内皮细胞和另一种抑制平滑肌细胞生长的适体在培养物中进行研究。 血管疾病的细胞和小鼠模型。 • Miller：I01 BX005450 是一项为期两年的优点，旨在确定细胞外是否失活 先前描述的合成 RNA 配体的组蛋白将防止急性炎症的发展 这些研究是在仓鼠模型中进行的。 新冠肺炎。 • Pisetsky：VA 优点，5I01BX003772-02，SLE 中自身抗原的细胞来源 • McMahon：VA Merit，VA BX-003478-01。SNO 转运调节红细胞的内皮粘附。 确定红细胞输出 SNO 和内皮细胞输入 SNO 的机制，形成信号传导 途径。 • Tighe：该实验室对空气污染改变肺部结构和功能的机制感兴趣。 • Murdoch：我们的研究涉及慢性炎症对合并症和终末器官疾病的影响， 包括气道疾病和药物滥用引起的炎症。