Upgrading the CVID Biosensor Core Facility with a Biacore 8K instrument

使用 Biacore 8K 仪器升级 CVID 生物传感器核心设施

• 批准号: 10176937
• 负责人: Dudley K. Strickland
• 金额: $ 43.89万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176937
• 关键词: Attention; Biology; Biosensor; Blood Vessels; Communities; Core Facility; Disease; Experimental Designs; Funding; Genes; Genomics; Home; Inflammatory; Label; Maryland; Measures; Minor; Molecular Weight; Peer Review; Physiological; Proteomics; Publications; Reproducibility; Research; Resources; Surface Plasmon Resonance; System; Technology; Universities; biomedical resource; gene product; innovation; instrument; medical schools

Project Summary/Abstract As tremendous advances have occurred in genomic biology, gene sequencing technologies, and proteomic technologies, attention is increasingly focusing on gene products, most of which function by interacting with other gene products. A comprehensive understanding of physiological systems requires an in-depth definition of quantitative interactions between molecules. Biosensor technologies provides a powerful approach to define these interactions in label-free systems. Here we seek funds to upgrade the Biosensor Core facility in the Center of Vascular and Inflammatory Diseases (CVID) at the University of Maryland School of Medicine by the purchase of a Biacore 8K instrument. The major users of this Core have extensive expertise employing surface plasmon resonance technology with over 45 peer-reviewed publications employing the technology. The new instrument (Biacore 8K) will be integrated into the CVID Biosensor Core Facility and with the Center for Innovative Biomedical Resources (CIBR), the Administrative home for the University of Maryland School of Medicine Core facilities, thus providing the resource for our growing research community. The Biacore 8K will enhance the research of the Major users and Minor users by increasing the throughput capacity, by enhancing a more reproducible and optimized experimental design, and by increasing the sensitivity for measuring the interaction of low molecular weight molecules.

项目概要/摘要 随着基因组生物学、基因测序技术和 蛋白质组学技术，人们的注意力越来越集中在基因产品上，其中大部分 通过与其他基因产物相互作用发挥作用。全面了解生理学 系统需要对分子之间的定量相互作用进行深入定义。生物传感器 技术提供了一种强大的方法来定义无标签系统中的这些相互作用。 在这里，我们寻求资金来升级血管和中心的生物传感器核心设施 炎症性疾病 (CVID) 在马里兰大学医学院购买 Biacore 8K 仪器。该核心的主要用户拥有广泛的专业知识 表面等离子共振技术，超过 45 篇同行评审出版物采用该技术 技术。新仪器（Biacore 8K）将集成到CVID生物传感器核心中 设施和创新生物医学资源中心 (CIBR)、行政中心 为马里兰大学医学院核心设施提供资源 我们不断壮大的研究社区。 Biacore 8K 将增强主要用户的研究 和小用户，通过提高吞吐能力，通过增强更具可重复性和 优化的实验设计，并通过提高测量相互作用的灵敏度 低分子量分子。