Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF/TOF) Mass Spectrometer

基质辅助激光解吸电离飞行时间 (MALDI-TOF/TOF) 质谱仪

• 批准号: 10175187
• 负责人: Bradley L. Nilsson
• 金额: $ 30.38万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175187
• 关键词: Applications Grants; Arts; Biopolymers; Certification; Chemistry; Data; Data Analyses; Dentistry; Elements; Engineering; Funding; Future; Health; Human; Human Biology; Laboratories; Lipids; Mass Spectrum Analysis; Methods; Minor; Molecular Weight; Oligonucleotides; Peptides; Polymers; Preparation; Proteins; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Sampling; Science; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time; Training; United States National Institutes of Health; Universities; base; college; cost; data acquisition; experience; improved; instrument; instrumentation; mass spectrometer; medical schools

Project Summary We request support for the purchase of a Jeol JMS-S3000 MALDI SpiralTOF spectrometer with TOF/TOF capability. The proposed instrument is an urgently needed replacement for an end-of-lifetime and inoperable Bruker Autoflex III MALDI-TOF instrument in the University of Rochester (UR) Department of Chemistry Instrumentation Facility (DCIF). Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) is a high- throughput mass spectroscopy (MS) method that is used for the analysis of high molecular weight analytes includes peptides, proteins, oligonucleotides, lipids, and polymers. No other MS instruments at UR are appropriate for the routine analysis of these types of samples. The DCIF is an open access instrument center and is available to all UR researchers in 45 departments and over 800 laboratories in the College of Arts, Sciences, and Engineering and in the School of Medicine and Dentistry. The proposed MALDI-TOF/TOF instrument will be available for direct use by all researchers that are trained and certified to operate the instrument by DCIF technicians. The Jeol JMS-S3000 MALDI SpiralTOF is a robust bench-top instrument that is ideal for use as a multi-user instrument. Sample preparation, data acquisition, and data analysis are straightforward. DCIF technical staff are highly experienced with MS instrumentation and are available to assist users as needed after training and certification. Access to this user-based instrument will enable rapid analysis that will accelerate the pace of and reduce the cost of research projects for which mass analyses of high molecular weight materials. The expanded TOF/TOF capability of the proposed instrument will enable acquisition of both mass and sequence information for biopolymer substrates. The proposed instrument is urgently needed to support research in 18 NIH-funded projects at UR. These projects are described in the proposal by 7 major users. Additional projects that will utilize the instrument are described by 5 minor users with 2 additional NIH-funded projects. Two of the minor users are emerging investigators who will rely on the instrument to obtain data that will lead to future NIH grant applications. It is anticipated that the enhanced TOF/TOF capability of the instrument will also broaden the biomedical user base of the instrument. The proposed instrument will become an integral element of the research infrastructure at UR for projects that are focused on understanding human biology and improving human health.

项目摘要 我们要求购买用TOF/TOF购买JEOL JMS-S3000 MALDI SPERALTOF光谱仪 能力。提出的仪器是迫切需要的临时和无法使用的材料 罗切斯特大学（UR）化学系的Bruker Autoflex III MALDI-TOF仪器 仪器设施（DCIF）。基质辅助激光解吸电离（MALDI）飞行时间（TOF）是高 吞吐量质谱法（MS）方法，用于分析高分子量分析物 包括肽，蛋白质，寡核苷酸，脂质和聚合物。 UR没有其他MS乐器 适合对这些类型样品的常规分析。 DCIF是一个开放访问仪器中心，可用于45个部门的所有研究人员， 艺术，科学和工程学院以及医学院的800多家实验室 牙科。拟议的MALDI-TOF/TOF仪器将可供所有研究人员直接使用 经过DCIF技术人员的培训和认证，可以操作该工具。 JEOL JMS-S3000 MALDI SPERALTOF是一个 强大的台式仪器，非常适合用作多用户仪器。样本准备，数据获取， 数据分析很简单。 DCIF技术人员在MS仪器和 培训和认证后可根据需要协助用户。访问此基于用户的仪器将 启用快速分析，将加快群众的速度并降低研究项目的成本 高分子重量材料的分析。提议的仪器的扩展的TOF/TOF能力将 能够获得生物聚合物底物的质量和序列信息。 迫切需要该拟议的工具来支持UR的18个NIH资助项目的研究。这些项目 7个主要用户在提案中描述。描述了将使用该仪器的其他项目 由5个额外的NIH资助项目提供的5个未成年人用户。两名未成年人是新兴的调查员 将依靠该工具获得将导致未来NIH赠款应用程序的数据。预计 仪器的增强功能还将扩大仪器的生物医学用户群。 拟议的工具将成为UR研究基础设施的组成部分 专注于了解人类生物学并改善人类健康。

项目成果

Co-crystal structures of HIV TAR RNA bound to lab-evolved proteins show key roles for arginine relevant to the design of cyclic peptide TAR inhibitors.

• DOI: 10.1074/jbc.ra120.015444
• 发表时间: 2020-12-04
• 期刊: The Journal of biological chemistry
• 作者: Chavali SS;Mali SM;Jenkins JL;Fasan R;Wedekind JE
• 通讯作者: Wedekind JE

Cellular Validation of a Chemically Improved Inhibitor Identifies Monoubiquitination on OTUB2.

• DOI: 10.1021/acschembio.3c00227
• 发表时间: 2023-09-15
• 期刊: ACS CHEMICAL BIOLOGY
• 影响因子: 4
• 作者: Gan, Jin;de Vries, Jelle;Akkermans, Jimmy J. L. L.;Mohammed, Yassene;Tjokrodirijo, Rayman T. N.;de Ru, Arnoud H.;Kim, Robbert Q.;Vargas, David A.;Pol, Vito;Fasan, Rudi;van Veelen, Peter A.;Neefjes, Jacques;van Dam, Hans;Ovaa, Huib;Sapmaz, Aysegul;Geurink, Paul P.
• 通讯作者: Geurink, Paul P.

Bone-Targeted Nanoparticle Drug Delivery System-Mediated Macrophage Modulation for Enhanced Fracture Healing.

• DOI: 10.1002/smll.202305336
• 发表时间: 2023-10
• 期刊: Small
• 影响因子: 13.3
• 作者: Baixue Xiao;Yuxuan Liu;Indika Chandrasiri;Emmanuela Adjei-Sowah;Jared Mereness;Ming Yan;Danielle S W Beno