Vectra Polaris microscope imaging system

Vectra Polaris 显微镜成像系统

• 批准号: 10175807
• 负责人: Mercedes Gonzalez-Juarrero
• 金额: $ 45.18万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175807
• 关键词: Affect; Biological; Biological Markers; Cell Communication; Colorado; Computer software; Core Facility; Development; Disease; Flow Cytometry; Fluorescence; Formalin; Foundations; Funding; Genus Mycobacterium; Image; Imaging technology; Immune response; Immunofluorescence Immunologic; Immunohistochemistry; Immunologic Markers; Laboratory Research; Lighting; Machine Learning; Microscope; Microscopic; Microscopy; Mission; Nature; Noise; Photobleaching; Pre-Clinical Model; Research; Research Personnel; Safety; Sampling; Scanning; Signal Transduction; Slide; Specimen; Speed; Stains; Structure; System; Technology; Time; Tissue Sample; Tissues; Training; United States National Institutes of Health; Universities; Vaccines; base; chemotherapy; digital; imaging system; improved; liquid crystal polymer; microscopic imaging; mycobacterial; operation; quantitative imaging; spatial relationship; user-friendly; vaccine candidate

PROJECT SUMMARY This proposal seeks funding for a Vectra® Polaris™ Automated Quantitative Imaging System (Polaris™), a state- of-the-art multispectral imaging microscope to serve multiple NIH-supported projects within the Mycobacteria Research Laboratories (MRL) at Colorado State University (CSU). Our collective mission is to develop effective chemotherapies and vaccines for mycobacterial diseases affecting millions worldwide. The biological activity and safety profile of candidate vaccines and treatments can only be fully understood through characterizing the host immune response, primarily by evaluating large networks of immune biomarkers through flow cytometry and immunohistochemistry. MRL researchers are seeking to visualize the spatial expression of immune biomarkers by multiplex staining of intact tissue samples obtained from preclinical models of mycobacterial diseases. Until now, microscopic analysis of biomarkers through confocal and traditional immunofluorescence was drastically limited by autofluorescence in tissue (especially in formalin fixed and diseased tissue) and low signal-to-noise ratios. The Polaris™ system has a unique set of microscopic capabilities that combined with the inForm® workstation allows simultaneous identification and quantification of multiple targets and their spatial relationships in tissue sections and microarrays. A critical aspect of the PolarisTM multispectral technology is the ability to readily distinguish autofluorescence from positive biomarker signal, improving the signal-to-noise ratio substantially. This allows for a more sensitive and accurate analysis of samples and enables true cell-to-cell interactions to be revealed. The system is user-friendly, and its automated scanning system allows for high speed digital and whole-slide scanning between 10-40x in brightfield or fluorescence. The nature of the illumination system significantly decreases deleterious effects such as photobleaching and photodamage, and thus specimens can be imaged for longer periods of time than with, for example, a confocal microscope. Furthermore, the advanced machine-learning based approaches of the included software automatically segment and quantitate tissue structures. The Polaris™ system will be incorporated into our existing microscopy core facility in order to expand access to trainees and other research groups at CSU and in Northern Colorado, while prioritizing access for the major users. Incorporation into the foundational core will sustain long term operation and provide consistent oversight and training. In summary, this state-of-the-art imaging technology is of critical importance to development of chemotherapies and vaccines by the MRL and will add value to multiple other NIH-supported projects at CSU.

项目概要 该提案为 Vectra® Polaris™ 自动定量成像系统 (Polaris™) 寻求资金，这是一个国家 最先进的多光谱成像显微镜，可为分枝杆菌内的多个 NIH 支持的项目提供服务 科罗拉多州立大学 (CSU) 研究实验室 (MRL) 我们的共同使命是提高效率。 影响全世界数百万人的分枝杆菌疾病的化学疗法和疫苗的生物活性和 只有通过表征宿主才能充分了解候选疫苗和治疗方法的安全性 免疫反应，主要通过流式细胞术评估免疫生物标志物的大型网络， MRL 研究人员正在寻求通过可视化免疫生物标志物的空间表达。 对从分枝杆菌疾病的临床前模型获得的完整组织样本进行多重染色。 通过共聚焦和传统免疫荧光对生物标志物进行显微分析受到以下因素的极大限制： 组织中的自发荧光（尤其是福尔马林固定的和患病的组织）和低信噪比。 Polaris™ 系统具有一套独特的微观功能，与 inForm® 工作站相结合，可实现 同时识别和量化组织切片中的多个目标及其空间关系 PolarisTM 多光谱技术的一个关键方面是能够轻松区分。 来自阳性生物标志物信号的自发荧光，大大提高了信噪比。 对样品进行更灵敏、更准确的分析，并能够揭示真实的细胞间相互作用。 系统用户友好，其自动扫描系统可实现高速数字和全玻片扫描 明场或荧光下 10-40 倍之间 照明系统的性质显着降低。 有害影响，例如光漂白和光损伤，因此标本可以成像更长时间 此外，基于先进机器学习的技术比使用共焦显微镜等要花费更多的时间。 所含软件的方法可自动分割和定量组织结构。 将被纳入我们现有的显微镜核心设施，以扩大受训人员和其他人员的接触范围 科罗拉多州立大学和北科罗拉多州的研究小组，同时优先考虑主要用户的访问。 基础核心将维持长期运营并提供一致的监督和培训。 这种最先进的成像技术对于化疗和疫苗的开发至关重要 并将为科罗拉多州立大学 NIH 支持的多个其他项目增加价值。