Lumick's C-Trap instrument for single-molecule analysis of macromolecular dynamics

Lumick 用于大分子动力学单分子分析的 C-Trap 仪器

• 批准号: 10175508
• 负责人: Maria Spies
• 金额: $ 60万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175508
• 关键词: Antiviral Agents; Biological; Biophysics; Cells; Chromatin; Crystallography; Data Analyses; Development; Ensure; Enzymes; Experimental Designs; Fluorescence Microscopy; Funding; Genome Stability; Human Resources; Individual; Institution; Iowa; Macromolecular Complexes; Maintenance; Medicine; Microfluidics; Molecular; Molecular Conformation; Molecular Machines; Nerve Degeneration; Nucleic Acids; Physiological; Process; Protein Analysis; Protein Dynamics; Proteins; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Resources; Structure; System; Time; Training; United States National Institutes of Health; Universities; Viral Genome; Visualization; Work; anti-cancer; antimicrobial; college; instrument; instrumentation; laser tweezer; macromolecule; operation; programs; single molecule; tool

PROJECT SUMMARY/ABSTRACT Investigators at the University of Iowa have a growing need to analyze macromolecular interactions and conformational dynamics at a single-molecule level. For over a decade, University of Iowa Carver College of Medicine Protein and Crystallography (P&C) Facility has been providing the University of Iowa investigators access to and help in operating of basic and state-of-the-art instrumentation for biophysical and structural analysis of proteins and nucleic acids. Addition of an instrument that will allow researchers to visualize and study biologically important molecules individually, in real time, and under physiological conditions will significantly enhance research programs at the University of Iowa. It will provide a much- needed tool for developing mechanistic understanding of how cells work and communicate, how molecular machines assemble and function. While several groups at the University of Iowa have built systems for single-molecule analysis in their labs, none of these instruments allows for simultaneous visualization and manipulation of biological macromolecules. Currently, there is also no single-molecule instruments centrally available to non-expert users at the University of Iowa. This proposal requests funds for a LUMICKS C-Trap(R) instrument, the first and only commercial instrument for single-molecule analysis of macromolecular interactions that combines high resolution optical tweezers, fluorescence microscopy and microfluidics, and therefore allows simultaneous manipulation and visualization of the macromolecular complexes. It is an important tool for the single- molecule multiparameter analysis of macromolecular interactions, assembly and function of molecular machines and conformational dynamics of proteins and nucleic acids. The C-Trap(R) will be housed at the P&C Facility whose personnel will be responsible for the instrument maintenance, user training, and will assist the investigators with experimental design, instrument operation and data analysis. The C-Trap(R) instrument will have an immediate impact on numerous NIH- funded research projects at the University of Iowa and collaborators, and will also serve as an important resource for our collaborators in regional institutions. The projects that will immediately benefit from this instrument include studies of the molecular processes that ensure genome stability, assembly and dynamics of multicomponent molecular machines, viral genome folding, chromatin organization, as well as enzymes that represent attractive targets for development of new anticancer, anti-neurodegeneration, anti-viral and antimicrobial therapies.

项目摘要/摘要 爱荷华大学的研究人员越来越需要分析大分子相互作用和 单分子级的构象动力学。十多年来，爱荷华大学卡佛学院 医学蛋白质和晶体学（P＆C）设施一直在提供爱荷华大学 研究人员可以使用并帮助运营基本和最先进的生物物理仪器 以及蛋白质和核酸的结构分析。添加可以允许研究人员的乐器 实时和生理下单独地可视化和研究生物学上重要的分子 条件将大大改善爱荷华大学的研究计划。它将提供一个很大的 所需的工具来发展对细胞如何工作和沟通的机械理解，如何 分子机组装和功能。爱荷华大学的几个小组已建成 在实验室中进行单分子分析的系统，这些仪器都不允许同时进行 生物大分子的可视化和操纵。目前，也没有单分子 爱荷华大学的非专家用户中心可用的仪器。 该提案要求资金用于Lumicks C-Trap（R）仪器，这是第一个也是唯一的商业商业 用于结合高分辨率的大分子相互作用的单分子分析的仪器 光学镊子，荧光显微镜和微流体，因此同时允许 大分子复合物的操纵和可视化。它是单个的重要工具 大分子相互作用，分子组装和功能的分子多组分分析 蛋白质和核酸的机器和构象动力学。 C-TRAP（R）将安置在P＆C设施中，其人员将负责该工具 维护，用户培训，并将协助调查人员实验设计，仪器 操作和数据分析。 C-TRAP（R）仪器将立即对许多NIH-产生影响 爱荷华大学和合作者资助的研究项目，也将成为重要的 我们在区域机构中的合作者的资源。将立即受益的项目 仪器包括对确保基因组稳定性，组装和的分子过程的研究 多组分分子机器，病毒基因组折叠，染色质组织的动力学 作为代表开发新抗癌，抗神经变性的有吸引力靶标的酶， 抗病毒和抗菌疗法。