Microbial Origin of Breath Volatile Metabolites

呼吸挥发性代谢物的微生物来源

• 批准号: 10171781
• 负责人: Andrew Leon Kau
• 金额: $ 20.84万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10171781
• 关键词: Anaerobic Bacteria; Animal Husbandry; Asthma; Autoimmune Diseases; Bacteria; Bile Acids and Salts; Biological; Biological Markers; Breath Tests; Chemical Structure; Clinical; Collection; Communicable Diseases; Coupling; Data; Diagnostic; Diagnostic tests; Disease; Ecology; Ensure; Exhalation; Genes; Germ-Free; Gnotobiotic; Health; Human; Hypersensitivity; In Vitro; Individual; Investigation; Knowledge; Lung; Malignant Neoplasms; Mass Spectrum Analysis; Mediating; Metabolic; Metagenomics; Methods; Microbe; Modeling; Mus; Obesity; Output; Participant; Patient Care; Pattern; Reproducibility; Research; Sampling; Shapes; Techniques; Testing; Time; Translating; Volatile Fatty Acids; Work; base; breath composition; dysbiosis; experience; gut bacteria; gut dysbiosis; gut microbes; gut microbiota; immune function; improved; innovation; insight; lipophilicity; metagenomic sequencing; microbial; microbial community; microbiota; novel; novel therapeutics; point of care testing; point-of-care diagnostics; pressure; success; tool; vapor; volatile organic compound

ABSTRACT Gut microbial communities influence multiple facets of human health, impacting common disorders such as obesity, autoimmune diseases, and allergies. These discoveries were enabled by the application of metagenomic sequencing and mass spectrometric techniques to human fecal samples, leading to remarkable insights into the interplay between gut microbial communities and human health. However, a major barrier to translating our growing knowledge about the gut microbiota and its functions to the patient-care setting is the difficulty in obtaining, storing, processing and analyzing fecal samples. Volatile organic compounds (VOCs) are present in exhaled breath and are produced by many microbes. By coupling a new method for analyzing the VOC composition of mouse breath samples with our gnotobiotic model, we find that breath VOC patterns reflect the gut microbial communities. Based on this preliminary data, our central hypothesis is that many VOCs observed in breath are produced by the gut microbiota and thus represent biomarkers of gut microbial community configuration and function. We will test our central hypothesis through the following specific aims: (1) Evaluate the extent to which gut microbial communities shape breath VOC profiles; and (2) Characterize human gut microbes that generate VOCs and demonstrate that microbe-produced VOCs are present in the breath of gnotobiotic mice. Our investigations will be significant, because we will greatly advance our fundamental understanding of the gut microbial contribution to breath volatile composition, and we will begin to identify specific, individual volatile-microbe correlations. Our work will therefore launch a new paradigm towards novel, non-invasive “breathalyzer” diagnostics to interrogate gut microbial dysbiosis.

抽象的 肠道微生物群落影响人类健康的多个方面，影响了常见疾病，例如 肥胖，自身免疫性疾病和过敏。这些发现是通过应用 宏基因组测序和质谱技术对人粪便样品，导致了显着的 洞悉肠道微生物群落与人类健康之间的相互作用。但是，一个主要的障碍 将我们对肠道菌群及其功能的知识转化为患者护理设置 是获得，存储，加工和分析粪便样品的困难。挥发性有机化合物 （VOC）出现在呼气的呼吸中，并由许多微生物产生。通过耦合一种新方法 通过我们的gnotobiotic模型分析鼠标呼吸样品的VOC组成，我们发现呼吸VOC 模式反映了肠道微生物群落。基于此初步数据，我们的中心假设是 呼吸中观察到的许多VOC都是由肠道微生物群产生的，因此代表了生物标志物 肠道微生物群落的配置和功能。我们将通过以下来检验我们的中心假设 具体目的：（1）评估肠道微生物群落塑造呼吸概况的程度； （2） 表征生成VOC的人类肠道微生物，并证明生产微生物的VOC是 出现在gnotobiotic小鼠的呼吸中。我们的调查将是重要的，因为我们将很大的进步 我们对肠道微生物对呼吸挥发性构成的贡献的基本理解，我们将开始 确定特定的单个挥发性微型相关性。因此，我们的工作将推出新的范式 迈向新型的无创“呼吸分析仪”诊断，以询问肠道微生物营养不良。

项目成果

Developmental progression of the nasopharyngeal microbiome during childhood and association with the lower airway microbiome.

儿童期鼻咽微生物组的发育进展及其与下呼吸道微生物组的关联。

• DOI: 10.1101/2023.09.18.23295747
• 发表时间: 2023
• 期刊: medRxiv : the preprint server for health sciences
• 作者: Hernandez-Leyva,ArielJ;Rosen,AnneL;Tomera,ChristopherP;Lin,ElainaE;Akaho,ElikplimH;Blatz,AllisonM;Otto,WilliamR;Logan,Joey;Young,LisaR;Harris,RebeccaM;Kau,AndrewL;John,AudreyROdom
• 通讯作者: John,AudreyROdom