Viral Testing Core

病毒检测核心

• 批准号: 10155603
• 负责人: Richard Grant
• 金额: $ 52.37万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10155603
• 关键词: AIDS therapy; Age; Animal Housing; Animals; Antibodies; Antigens; Biological Assay; Biomedical Research; Breeding; Cercopithecine Herpesvirus 1; Coculture Techniques; Cytomegalovirus; Derivation procedure; Detection; Diagnostic Services; Disease; Ensure; Enzyme-Linked Immunosorbent Assay; Epitopes; Flavivirus; Future; Genes; Genome; Goals; Grant; Health; Herpesviridae; Herpesvirus 1; Homologous Gene; Housing; Immune; In Vitro; Infectious Agent; International; Laboratories; Location; Macaca; Macaca nemestrina; Maintenance; Measles; Methods; MicroRNAs; Noise; Nucleic Acids; Parasites; Pathogen detection; Peer Review; Plasma; Predictive Value; Preparation; Prevalence; Price; Primates; Proteins; Reagent; Recombinants; Recommendation; Reporting; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Retroviridae; Risk; Running; SIV; Sampling; Schedule; Scientist; Sensitivity and Specificity; Serology; Serotyping; Services; Specificity; Testing; Time; Training; Trypanosoma; Trypanosoma cruzi; Vaccines; Veterinarians; Viral; Viral Genome; Virus; Virus Diseases; Work; animal care; base; biological specimen archives; cost effective; detection assay; detection method; emerging pathogen; env Gene Products; germ free condition; improved; innovation; latent infection; medical specialties; member; multiplex assay; nano-string; nonhuman primate; pathogen; screening; social; success; testing services; virus core; working group

Project Summary/Abstract: Viral Testing Core The WaNPRC Primate Diagnostic Services Laboratory (PDSL) performs the pathogen detection assays that are integral to the establishment and maintenance of specific pathogen-free (SPF) colonies. The laboratory also responds to new pathogen concerns from the various fields of NHP research by assembling or producing the reagents and controls and then developing and validating new assays on-demand. PDSL works collaboratively with the other NPRC pathogen-detection laboratories to ensure our assays are equivalent and comparable. A biannual proficiency panel, of which PDSL contributes and participates as part of the Breeding Colony Management/Pathogen-Detection Working Group, helps ensure peer-review and rigor and reproducibility in our assays. As Core 2 of the WaNPRC SPF U42 grant for our Macaca nemestrina breeding colonies, the PDSL works closely with the veterinarians and other animal care staff to ensure that the appropriate tests are run at the right time and reported in a timely manner to help guide the decisions on housing and breeding. The pathogens of highest concern are the retroviruses SRV, STLV and SIV as well as the alphaherpesvirus, Macacine herpesvirus 1 (McHV1 a.k.a. herpes B virus). Approximately 740 pigtail macaques will be tested semi-annually or quarterly, depending on age and social housing. PDSL will also train and advise on topics of NHP pathogens and detection methods, coordinate timing of test schedules, run tests according to priority of the requestors, report results quickly and accurately, make recommendations for animal housing and moves based on pathogen status, and archive specimens for additional tests that may occur in the future. In the past 3 years, our center has made good progress in achieving SPF status on large groups of breeding age animals and part of that progress is attributable to refinements in the laboratory assays to give greater specificity and fewer false reactive samples. New Aims for the Viral Testing Core in this application build on the improvements achieved and investigate new protein and nucleic acid targets that can be selected to add to our multiplexed screening assays. In completing the goals of this grant, our laboratory and our team members in Cores 1 and 3 seek to make strong contributions to biomedical research by maintaining a robust colony of SPF pigtail macaques.

项目摘要/摘要： 病毒测试核心 WANPRC灵长类动物诊断服务实验室（PDSL）进行病原体检测测定法 是特定无病原体（SPF）菌落的建立和维持不可或缺的一部分。实验室 还通过组装或生产来应对NHP研究各个领域的新病原体关注 试剂和控制，然后开发并验证按需新测定。 PDSL起作用 与其他NPRC病原体检测实验室合作，以确保我们的测定相同，并且 可比。 PDSL的一年一次熟练面板作为育种的一部分贡献并参与 殖民地管理/病原体检测工作组，有助于确保同行评审和严格 我们的测定中的可重复性。作为WANPRC SPF U42赠款的核心2 殖民地，PDSL与兽医和其他动物护理人员紧密合作，以确保 适当的时间进行适当的测试，并及时进行报告，以帮助指导决策 住房和繁殖。最高关注的病原体是逆转录病毒SRV，STLV和SIV以及 Alphaherpesvirus，themacine疱疹病毒1（MCHV1又称疱疹病毒）。大约740根辫子 猕猴将在半年度或每季度进行测试，具体取决于年龄和社会住房。 PDSL也将训练 并就NHP病原体和检测方法的主题提供建议，坐标测试时间表，运行测试 根据请求者的优先级，报告结果迅速准确，为动物提出建议 基于病原体状态的外壳和移动，以及可能发生的其他测试的档案标本 未来。在过去的三年中，我们的中心在达到SPF状态方面取得了良好的进步 繁殖年龄的动物和部分进展归因于实验室测定中的改进 更特异性和更少的假反应样品。在本应用程序中的病毒测试核心的新目标 建立在可以选择的新蛋白质和核酸靶标的改进的基础上 添加到我们的多重筛选测定中。在完成这笔赠款的目标时，我们的实验室和我们的团队 核心1和3中的成员通过维护强大的 SPF紫尾猕猴的殖民地。