Core D - Immune Monitoring Core

核心 D - 免疫监测核心

基本信息

批准号：
10153660
负责人：
MIRIAM MERAD
金额：
$ 30万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10153660
关键词：
Address B-Lymphocytes Biological Assay CD8-Positive T-Lymphocytes Cell physiology Cells Cellular Assay Cellular Compartment Analysis Clinical Collaborations Cytometry Data Data Analyses Dendritic Cells Dengue Dengue Infection Dengue Vaccine Dengue Virus Development Disease Disease Outcome Enrollment Exposure to Fingerprint Future Gene Expression Profiling Generations Genes Genetic Genetic Transcription Genomics Goals Helper-Inducer T-Lymphocyte Human Human Characteristics Immune Immune response Immunity Immunocompetence Immunologic Monitoring Immunology Impairment In Vitro Individual Infection Innate Immune Response Longitudinal Studies Mass Spectrum Analysis Methods Modeling Myelogenous Myeloid Cells Natural Immunity Nicaragua Nicaraguan Office of Administrative Management Outcome Pathogenicity Patients Peptides Peripheral Blood Mononuclear Cell Phenotype Plasma Procedures Proteomics Sampling Serology Serotyping Standardization System Systems Analysis Systems Biology T cell response T-Lymphocyte Technology Testing Time Vaccinated Vaccination Vaccine Clinical Trial Vaccines Woman adaptive immune response adaptive immunity antiviral immunity base cohort cost data management immune function immunological status immunoregulation in vivo member men monocyte novel pathogenic virus response vaccination outcome

项目摘要

The Human Immune Monitoring Core will characterize phenotypic and functional, ex vivo and in vivo signature as well as analyses of innate and adaptive immune responses to DENV infection and challenges at various time points whereas information on humoral immune responses to DENV will be obtained from the longitudinal studies of infected patients described project 1. Together, these validated assays will generate data to be integrated with results from the genomic and proteomic cores, and contribute to a DENV signature with clinical value. We anticipate that advances in mass spectrometry technologies and sample miniturization procedures may increase the sensitivity of global and functional analyses and may reduce the amount of sample required for these analyses allowing to extend innate immune competence studies to infected and vaccinated patients further refining the identification of a signature correlated with clinical status upon DENV infection, vaccine outcome or DC pathogenicity. These assays comprise the groundwork for future development of new immune enhancing strategies to promote the development of a protective immune response.

人类免疫监测核心将表征表型和功能，离体和体内签名以及对DENV感染的先天和适应性免疫反应的分析以及各个时间点的挑战，而有关体液免疫反应的信息 DENV将从受感染患者的纵向研究中获得，描述了项目1。这些经过验证的测定将共同生成数据，以与基因组和蛋白质组学核心，并有助于具有临床价值的DENV签名。我们预计质谱技术和样品销售的进步程序可能会提高全球和功能分析的敏感性，并可能降低这些分析所需的样本数量，以扩展先天免疫能力对感染和接种疫苗的患者的研究进一步完善了签名的鉴定与DENV感染，疫苗结果或DC致病性时，与临床状况相关。这些测定法是未来开发新免疫增强的基础促进保护性免疫反应的策略。