ROLE OF STEROID HORMONES ON THE CELLULAR COMMUNICATION : MOLECULAR, MORPHOLOGICAL, PHYSIOLOGICAL AND BEHAVIORAL MULTIDICIPLINARY APPROACH

类固醇激素对细胞通讯的作用：分子、形态学、生理学和行为多学科方法

• 批准号: 10044314
• 负责人: KAWATA Mitsuhiro
• 金额: $ 4.22万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044314/
• 关键词: SUBCELLULAR LOCALIZATION; STEROID HORMONE RECEPTORS; GFP; REAL-TIME IMAGING; LIGAND-DEPENDENT TRANSCRIPTION FACTOR; GLUCOCORTIOCOLDS; SEX HORMONE; NUCLEOCYTOPLASMIC TRANSLOCATION; 性ホルモン; エストロゲンレセプター; 生後発育; アポトーシス; 強制発現; 視索前野; 雌雄差; GEP

The subcellular distribution of the steroid hormone receptors has not yet been established and controversial : different methods showed different results, I.e. each type of receptor is in cytoplasmic or nuclear or both in the absence of the ligand. To elucidate the localization of receptors of adrenal steroids and estrogen, we carried out an immunocytochemistry with the use of specific antibodies and an imaging analysis by green fluorescent protein (GFP) chimera system. Without the ligand glucocorticoid receptor (GR) is in the cytoplasmic and mineralocorticoid receptor (MR) is both in the cytoplasmic and nuclear, whereas estrogen receptor (ER) a and b are both in the nucleus. Ligand induces the accumulation of all these receptors in the nucleus. The ligand-induced movement of these nuclear receptors from inactive states depends on each type of receptor.

类固醇激素受体的亚细胞分布尚未确定并存在争议：不同的方法显示不同的结果，即在没有配体的情况下，每种类型的受体都存在于细胞质或细胞核中或两者中。为了阐明肾上腺类固醇和雌激素受体的定位，我们使用特异性抗体进行了免疫细胞化学分析，并通过绿色荧光蛋白（GFP）嵌合系统进行了成像分析。没有配体时，糖皮质激素受体（GR）位于细胞质中，盐皮质激素受体（MR）位于细胞质和细胞核中，而雌激素受体（ER）a和b均位于细胞核中。配体诱导所有这些受体在细胞核中积累。配体诱导的这些核受体从非活性状态的运动取决于每种类型的受体。

项目成果

Lu H., Yuri K., Ito T., Yoshimoto K., Kawata M.: "The effects of oestrogen and progesterone on serotonin and its metabolite in the lateral septum, medial preoptic area and ventromedial hypothalamic nucleus of female rats"J.Neuroendocrinology. 10. 919-926

Lu H.，Yuri K.，Ito T.，Yoshimoto K.，Kawata M.：“雌激素和孕激素对雌性大鼠外隔、内侧视前区和腹内侧下丘脑核血清素及其代谢物的影响”J。

Hu Z., Ito T., Yuri K., Xie,C., Ozawa H., Kawata M.: "In vivo time course of morphological changes and DNA degradation during the degeneration of castration-induced apoptotic cells"Cell Tissue Res.. 294. 153-160 (1998)

Hu Z.、Ito T.、Yuri K.、Xie,C.、Ozawa H.、Kawata M.：“去势诱导凋亡细胞变性过程中形态变化和 DNA 降解的体内时间过程”细胞组织研究。

Matsushita, H. et al: "Changes in serotonergic neurons in the brain of pyrithiamine-induced acute thiamine-deficient mice"Acta Neuropathol. 98. 614-621 (1999)

Matsushita, H. 等人：“吡硫胺诱导的急性硫胺素缺乏小鼠大脑中血清素能神经元的变化”Acta Neuropathol。

森田規之,伊藤隆男,河田光博: "脱アイソトープ実践プレトコール,カラーin situハイブリダイゼーション" 秀潤社,東京, 269-299 (1998)

Noriyuki Morita、Takao Ito、Mitsuhiro Kawata：“同位素去除实践、彩色原位杂交” Shujunsha，东京，269-299（1998）

Matsushita,H., Takeuchi,Y., Kosaka,K., Fushiki, S., Kawata,M., Sawada, T.: "Changes in serotonergic neurons in the brain of pyrithiamine-induced acute thiamine-deficient mice"Acta Neuropathol. 98. 614-621 (1999)

Matsushita，H.，Takeuchi，Y.，Kosaka，K.，Fushiki，S.，Kawata，M.，Sawada，T.：“吡硫胺诱导的急性硫胺素缺乏小鼠大脑中血清素能神经元的变化”Acta Neuropathol