DYNAMICS AND REGULATION OF NUCLEAR RECEPTORS OF LIPID SOLUBLE MOLECULE IDENTIFIED BY USING GFPS
GFPS鉴定脂溶性分子核受体的动力学和调控
基本信息
- 批准号:13470005
- 负责人:
- 金额:$ 10.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Nuclear receptors which include glucocorticoid receptor (GR), mineralocorticoid receptor (MR), estrogen receptor (ER) α and β, and androgen receptor (AR) are ligand-dependent transcriptional factor. With the advent of green fluorescent protein (GFP) and its color variants, the subcellular distribution of many nuclear receptors has been found to be much more dynamic than previously thought. GFP studies showed that microtubules are not involved in the translocation of GR and MR from cytoplasm to nucleus, but that HSP90 and importin system, at least in part, are strongly relevant to this translocation. Hormonal stimulation induced intranuclear receptor distribution from a homogeneous pattern to a heterogeneous dot-like image. GR and MR showed a partial colocalization at 30 min and nearly complete colocalization at 60 min after addition of corticosterone. The occurrence of FRET between GR and MR was observed in the nucleus. FRAP analysis showed that cytoplasmic GR was dynamic and nuclear GR was also mobile, but its mobility was different. Upon estradiol treatment ER α and β in the same cell were relocalized to show discrete pattern, and they were localized at the same discrete cluster, suggesting that both subtypes of Ers were bound to the same nuclear sites. In the absence of the ligand, any significant relationship between the diffuse distribution of Ers and the discrete distribution of Brg-1 was not observed. In the presence of the estradiol, however, the discrete staining pattern of ER α and β were mostly overlapped with Brg-1, indicating that most of the Ers clusters are involved in the chromatin remodeling machinery.
包括糖皮质激素受体(GR),矿物皮质激素受体(MR),雌激素受体(ER)α和β以及雄激素受体(AR)的核受体是配体依赖性的转录因子。随着绿色荧光蛋白(GFP)及其颜色变体的发展,许多核受体的亚细胞分布比以前想象的要动态性得多。 GFP的研究表明,微管与从细胞质到核的GR和MR的翻译不涉及,但是HSP90和Importin System至少部分与此转运密切相关。激素刺激从均匀模式到异质点样图像引起了插图的接收器分布。 GR和MR在30分钟时显示部分共定位,在加入皮质酮后60分钟几乎完全共定位。在核us中观察到了GR和MR之间的FRET的发生。 FRAP分析表明,细胞质GR是动态的,核GR也是可移动的,但其迁移率不同。在雌二醇处理后,将同一细胞中的ERα和β重新定位以显示离散的模式,并且它们以相同的离散簇定位,这表明ERS的两个亚型都与同一核位点结合。在没有配体的情况下,未观察到ER的扩散分布与BRG-1的离散分布之间的任何显着关系。然而,在雌二醇的存在下,ERα和β的离散染色模式主要与BRG-1重叠,表明大多数ERS簇参与染色质重塑机械。
项目成果
期刊论文数量(64)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nishi M.: "Dynamic changes in subcellular localization of mineralocorticoid receptor in living cells : In comparison with glucocorticoid receptor using dual-color labeling with green fluorescent protein spectral variants"Mol.Endocrinol.. 15. 1077-1092 (20
Nishi M.:“活细胞中盐皮质激素受体亚细胞定位的动态变化:与使用绿色荧光蛋白光谱变体双色标记的糖皮质激素受体相比”Mol.Endocrinol.. 15. 1077-1092 (20
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Nakamura N., Fujita H., Kawata M.: "Effects of gonadectomy on immunoreactivity for choline acetyltransferase in the cortex, hippocampus, and basal forebrain of adult male rats"Neuroscience. 109. 473-485 (2002)
Nakamura N.、Fujita H.、Kawata M.:“性腺切除术对成年雄性大鼠皮质、海马和基底前脑中胆碱乙酰转移酶免疫反应性的影响”神经科学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kawata M., Matsuda K., Nishi M., Ogawa H., Ochiai I.: "Intracellular dynamics of steroid hormone receptor"Neurosci. Res.. 40. 197-203 (2001)
川田 M.、松田 K.、西 M.、小川 H.、落合 I.:“类固醇激素受体的细胞内动力学”Neurosci。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
小澤一史: "ストレスとグルココルチコイド受容体"脳の科学. 24. 219-226 (2002)
Kazushi Ozawa:“压力和糖皮质激素受体”《脑科学》24. 219-226 (2002)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ogawa H., Nishi M., Kawata M.: "Localization of nuclear coactivators p300 and steroid receptor coactivator 1 in the rat hippocampus"Brain Res.. 890. 197-202 (2001)
小川 H.、西 M.、川田 M.:“大鼠海马中核辅激活因子 p300 和类固醇受体辅激活因子 1 的定位”Brain Res.. 890. 197-202 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KAWATA Mitsuhiro其他文献
KAWATA Mitsuhiro的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KAWATA Mitsuhiro', 18)}}的其他基金
Structural analysis of molecular and behavioral neuroendocrinology on the sexual difference and hormonal action in the nervous system
分子和行为神经内分泌学对神经系统性别差异和激素作用的结构分析
- 批准号:
24300128 - 财政年份:2012
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Steroid hormones and their receptor interaction by Raman spectrometer analysis
通过拉曼光谱仪分析类固醇激素及其受体相互作用
- 批准号:
22659040 - 财政年份:2010
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Interaction of genomic and non-genomic actions of hormones on the nervous system
激素对神经系统的基因组和非基因组作用的相互作用
- 批准号:
20240036 - 财政年份:2008
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Environmental Effects on the Dynamism of Receptors of Lipophilic Signal Molecules in the Brain and its Functional Significance
环境对脑内亲脂信号分子受体动态的影响及其功能意义
- 批准号:
16200026 - 财政年份:2004
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
MOLECULAR DYNAMICS OF RECEPTORS OF LIPID SOLUBLE SIGNAL MOLECULES
脂溶性信号分子受体的分子动力学
- 批准号:
11470006 - 财政年份:1999
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
TRANSCRIPTION FACTOR INTERACTIONS REVEALED BY FRET
FRET 揭示的转录因子相互作用
- 批准号:
11358013 - 财政年份:1999
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
ROLE OF STEROID HORMONES ON THE CELLULAR COMMUNICATION : MOLECULAR, MORPHOLOGICAL, PHYSIOLOGICAL AND BEHAVIORAL MULTIDICIPLINARY APPROACH
类固醇激素对细胞通讯的作用:分子、形态学、生理学和行为多学科方法
- 批准号:
10044314 - 财政年份:1998
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Effects of perinatal steroid hormonal changes on the cytoarchitecture of brain
围产期类固醇激素变化对大脑细胞结构的影响
- 批准号:
09480214 - 财政年份:1997
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Steroid receptors-multiforms and their significance on brain differentiation
多种形式的类固醇受体及其对大脑分化的意义
- 批准号:
07458257 - 财政年份:1995
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
AN APPLICATION OF IN SITU HYBRIDIZATION AND ANTISENSE OLIGONUCLEOTIDE METHOD TO VISUALIZE AND MANIPULATE GENE EXPRESSION OF STEROID HORMONE RECEPTOR PROTEINS IN THE NERVOUS SYSTEM
应用原位杂交和反义寡核苷酸方法可视化和操纵神经系统中类固醇激素受体蛋白的基因表达
- 批准号:
05454674 - 财政年份:1993
- 资助金额:
$ 10.3万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)