Metabolic evaluation using in vivo NMR spectroscopy for individual organs under serious conditions associated with reductive liver mitochondrial state

在与还原肝线粒体状态相关的严重条件下使用体内核磁共振波谱对各个器官进行代谢评估

基本信息

批准号：
09307024
负责人：
OZAWA Kazue
金额：
$ 18.88万
依托单位：
SHIGA UNIVERSITY OF MEDICAL SCIENCE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09307024/
关键词：
AKBR liver mitochondria systemic care in vivo NMR spectroscopy energy metabolism アポトーシス ^<31>P NMR ^<13>C NMR

项目摘要

Even in severe circulatory insufficient conditions during hemorrhage in jaundiced rats, the phosphoenergetic level in the myocardium was maintained fairly well. In such conditions, arterial ketone body ratio (AKBR) decreased and it was found to be a potential parameter to evaluate the systemic metabolic state. We also applied ィイD131ィエD1P- and ィイD113ィエD1C-NMR spectroscopy together to examine the metabolic state of the liver. Furthermore, the metabolic states of individual organs, such as liver, heart, brain and skeletal muscles, were investigated with NMR spectroscopy using hemorrhagic shock, endotoxic shock, myocardial infarction, brain infarction, epilepsy and diabetic animal models. We incorporated ultra-fast echo planar imaging (EPI) technique into ィイD11ィエD1H-NMR spectroscopic imaging. We have already reported fast ィイD113ィエD1C metabolite mapping derived from ィイD113ィエD1C-glucose in rat focal brain ischemia.These NMR spectroscopic techniques were also applied to the clinical cases. In patients with diabetic gangrene, we found metabolic abnormalities in the skeletal muscles associated with increased pII. Such findings were clearly different from that with ischemia. For heart disease, ィイD131ィエD1P NMR spectroscopy during hand-grip exercise was found to be useful to judge the viability of myocardium. In brain research ィイD11ィエD1H NMR spectroscopy was carried out for the judgment of malignant potential of maningioma, the pathophysiologic condition of myotonic dystrophy, spectroscopy during photic stimulation was used for the prediction of the effects of lithium or electrical stimulation therapy for bipolar disorder. Fast NMR spectroscopic imaging technique could be also applicable with clinical cases.

即使在果酱大鼠出血期间发生严重的循环状态下，心肌的磷酸烯水平也得到了很好的维持。在这种情况下，动脉酮体比率（AKBR）得到了改善，发现它是评估系统性代谢状态的潜在参数。我们还一起应用了D131 D1P和D113 D1C-NMR光谱，以检查肝脏的代谢状态。此外，使用出血性休克，内毒性休克，心肌违规，脑违规，癫痫病和糖尿病动物模型，研究了单个器官的代谢状态，例如肝脏，心脏，脑和骨骼肌。我们将超快速回声平面成像（EPI）技术纳入EI D11H-NMR光谱成像中。我们已经报道了大鼠局灶性脑部缺血中D113E D1C-葡萄糖衍生得出的快速EI D113E D1C代谢物映射。这些NMR光谱技术也应用于临床病例。在糖尿病坏疽的患者中，我们发现与PII增加有关的骨骼肌中的代谢异常。这种发现与缺血明显不同。对于心脏病，发现手动运动过程中的D131E D1P NMR光谱对于判断心肌的生存能力很有用。在脑研究中，D11E D1H NMR光谱是为了判断性手术瘤的恶性潜力，肌发育症的病理生理状况，光刺激过程中的光谱刺激的光谱刺激用于预测双极色素障碍的木化或电刺激治疗的作用。快速的NMR光谱成像技术也可能适用于临床病例。