Studies on the origin of the Japanese based on the analysis of MVR-PCR alleles in asian populations

基于亚洲人群MVR-PCR等位基因分析的日本人起源研究

基本信息

批准号：
09470123
负责人：
KATSUMATA Yoshinao
金额：
$ 6.14万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470123/
关键词：
Population Genetics Minisatellite D7S21 D1S8 D16S309 MVR-PCR Origin of the Japanese

项目摘要

Last year, we could obtain DNA specimens of 100 Chinese individuals, and investigated the allele frequencies of three polymorphic sites in DNA flanking D1S8 (MS32) and D7S21 (MS31A) loci. Haplotype frequencies in Chinese were compared with those in Japanese and Caucasians which had been already reported. Distribution of haplotypes in Chinese is not significantly different from that in Japanese, but significantly different from that in Caucasian. Allele-specific MVR-PCR based on the selective amplification of single allele from total genomic DNA using allele-specific PCR primers was then performed. Although MVR-PCR allele codes are highly polymorphic, the similarity of the internal structure can be analysed by dot matrix analysis. Furthermore, different alleles can have related flanking haplotypes, presumably due to sharing recent common ancestors. When groups of aligned MS32 alleles or MS31A alleles were made by dot matrix analysis, we have already shown that Caucasian, Aflican black and Japanese have strong tendency to form unique groups composed of mostly the same spieces. In this study, we found that Chinese alleles often had the same motif as that of Japanese alleles resulting in the groups consist of only Japanese and Chinese alleles. Thus, using MVR-PCR allele codes and flanking haplotypes, we could show that Chinese alleles were chosely related to Japanese alleles. Allele mapping of D16S309 (MS205) was also performed with Japanese DNA specimens. We can usually obtain MVR-PCR maps of whole alleles at this locus because the length of the most alleles is short. We found that Japanese had rather short alleles, and their internal structures were somewhat diffrenent from those of Caucasians. We also showed the usufullness of MVR-PCR mapping for paternity testings using practical cases.

去年，我们可以获得100个中国个体的DNA标本，并研究了DNA侧面D1S8（MS32）和D7S21（MS31A）基因座的三个多态位点的等位基因频率。将中国人的单倍型频率与已经报道的日本和高加索人的频率进行了比较。中文中单倍型的分布与日本的分布没有显着差异，但与白种人的分布不同。然后，使用等位基因DNA使用等位基因特异性PCR引物对单个等位基因的选择性扩增基于等位基因特异性MVR-PCR。尽管MVR-PCR等位基因代码高度多态，但可以通过DOT矩阵分析来分析内部结构的相似性。此外，不同的等位基因可能具有相关的侧翼单倍型，这可能是由于共享最近的共同祖先。当通过DOT矩阵分析制成一组对齐的MS32等位基因或MS31A等位基因时，我们已经表明，高加索人，露天黑人和日本具有强烈的趋势，它们倾向于形成主要由相同的香料组成的独特组。在这项研究中，我们发现中国等位基因通常与日本等位基因相同的图案，导致群体仅由日本和中国等位基因组成。因此，使用MVR-PCR等位基因代码和侧翼单倍型，我们可以证明中国等位基因与日本等位基因相关。日本DNA标本还进行了D16S309（MS205）的等位基因映射。通常，我们可以在此基因座获得整个等位基因的MVR-PCR图，因为最大等位基因的长度很短。我们发现日本人的等位基因相当短，其内部结构与高加索人的结构有些不同。我们还展示了使用实际情况进行亲子鉴定的MVR-PCR映射的USUFLES。