STUDIES ON CONTROL OF ENZYME REANTIONS PERFORMED BY SUPRAMOLECULAR NANO-REACTORS

超分子纳米反应器酶反应控制研究

基本信息

批准号：
09450332
负责人：
KIKUCHI Jun-ichi
金额：
$ 3.2万
依托单位：
NARA INSTITUTE OF SCIENCE AND TECHNOLOGY (1998)Kyushu University (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

1. Supramolecular assemblies as nano-reactors being capable of controlling enzymic activity were constituted in combination with an artificial receptor, an enzyme, and a bilayer-forming lipid.2. Synthetic peptide lipids having an amino acid residue interposed between a polar head moiety and a hydrophobic double-chain segment formed morphologically stable bilayer vesicles as nano-scale reaction vessels.3. NADH-dependent lactate dehydrogenase was bound to the vesicular surface, mainly through electrostatic interactions, keeping a conformation of the active site in aqueous media.4. The steroid cyclophane composed of three functional components, a cyclophane ring, four bile acid moieties, and four lysine residues connecting them, was prepared as an artificial receptor. When the receptor was embedded in the bilayer membrane, this molecule behaved as a molecular switch of the nano-reactor by performing double signal recognition.5. A bile acid derivative having an amino group acted as an artificial cell-surface receptor capable of activating lactate dehydrogenase by performing double signal recognition and phase reorganization in a synthetic bilayer membrane. Analogous behavior was also observed for an artificial transmembrane receptor having an azobenzene moiety.6. Aromatic aldehydes such as 1 -hydroxy-2-naphthaldehyde and cupper(II) ions were effective external and second messenger signals, respectively, for these artificial receptors.

1。超分子组件作为能够控制酶活性的纳米反应器，由人工受体，酶和双层形成脂质组合组成。2。具有氨基酸残基的合成肽脂质在极性头部部分中插入的氨基酸残基和疏水双链节段形成形态稳定的双层囊泡作为纳米级反应容器。3。 NADH依赖性乳酸脱氢酶主要通过静电相互作用与囊泡表面结合，在水性培养基中保持活性位点的构象。4。类固醇环烷由三个功能成分，一个环烷环，四个胆汁酸部分和连接它们的四个赖氨酸残基组成，作为人造受体制备。当将受体嵌入双层膜中时，该分子通过执行双信号识别而表现为纳米反应器的分子转换。5。具有氨基的胆汁酸衍生物充当人造细胞表面受体，能够通过在合成双层膜中进行双信号识别和相位重组来激活乳酸脱氢酶。对于具有偶氮苯部分的人工跨膜受体也观察到了类似行为。6。对于这些人造受体，芳香醛，例如1-羟基-2-萘醛和库珀（II）离子分别是有效的外部和第二信号信号。