DEVELOPMENT OF SYNTHETIC LIPOSOMES WITH CERAMIC SURFACE AND THEIR NOVEL FUNCTIONS

陶瓷表面合成脂质体的开发及其新功能

基本信息

批准号：
12450364
负责人：
KIKUCHI Jun-ichi
金额：
$ 9.28万
依托单位：
NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

The aim of this research project is to develop liposomal vesicies having ceramic surfaces as novel organic-inorganic hybrid nanomaterials. Liposomes, organic bilayer vesicles formed with lipids such as biomembrane-forming phospholipids, have been employed as biomembrane models and drug carriers. On the other hand, in recent years, much attention has been focused on unique functions of organic-inorganic hybrids which were derived from fusion of characteristics of organic materials with those of inorganic ones. On these grounds, we designed and prepared a novel organic-inorganic hybrid vesicle, which is a liposome coated with silica ceramics, and investigated in their novel functions. Topics in this research are as follows.(1) Development of organic-inorganic hybrid vesicles "Cerasomes"Liposome-forming double-chain lipids each having a trialkoxysilyl head moiety were designed and synthesized. In aqueous media, liposomes with inorganic frameworks like a silica ceramic on the surface were … More successfully prepared through hydrolysis of the head moiety and followed condensation of the silanol head, and self-assembling under appropriate conditions. We named the hybrid vesicle "Cerasome".(2) Physical properties, surface modification, and functionalization of CarecomesMorphological stability of the Cearsomes was drastically increased as compared with the conventional liposomes, while the Cerasomes possessed basic physical properties characteristic to the liposomes, such as compartmentation ability and gel-to-liquid-crystallibne phase transition behavior. Functionalization of the Cerasomes was achieved by surface modification of the nanohybrids.(3) Three-dimensional organization of CerasomesRemarkable morphological stability of the Cerasomes led us to construct hierarchical and three-dimensional architectures of the liposomal vesicles on substrate by employing layer-by-layer assembling technique. Thus, the results of the present study would provide us a new promising research field of the liposome chemistry for aiming to develop artificial multicellular systems and artificial organs. Less

该研究项目的目的是开发具有陶瓷表面的脂质体囊泡，作为新型有机无机杂交纳米材料。脂质体是由脂质形成的有机双层蔬菜，例如生物膜形成的磷脂，已被聘为生物膜模型和药物载体。另一方面，近年来，已经关注了有机无机杂种的独特功能，这些功能是从有机材料与无机材料的特征融合中得出的。以这些理由，我们设计并制作了一种新型的有机无机杂种囊泡，该囊泡是涂有二氧化硅陶瓷的脂质体，并在其新功能中进行了研究。这项研究中的主题如下。（1）开发有机无机杂化蔬菜“ Cerasomes”脂质体形成的双链脂质，每个脂质都设计并合成了试验性的koxysilyl头部部分。水性培养基，具有无机框架的脂质体，例如表面上的二氧化硅陶瓷……通过水解头部部分的水解并随后凝结硅酚头，并在适当的条件下进行自组装。（2）与常规脂质体相比，杂种蔬菜“ Cerasome”。通过纳米杂交的表面修饰来实现CERASOMES的功能化。（3）CERASOMES的CERASOMESREMESREMESRAMABLEMABLEMABLEMABLY形态稳定性的三维组织使我们通过采用层状层面层的层状囊泡来构建层次和三维体系结构，并采用层层的层层层层层层。这是本研究的结果将为我们提供脂质体化学的新希望研究领域，以开发人工多细胞系统和人造器官。较少的