Functional studies of presynaptic proteins in neurotransmitter release

突触前蛋白在神经递质释放中的功能研究

• 批准号: 09480225
• 负责人: MOCHIDA Sumiko
• 金额: $ 6.72万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480225/
• 关键词: synaptic vesicles; neurotransmitter; exocytosis; prosynaptic proteins; CaィイD12+ィエD1-binding proteins; CaィイD12+ィエD1 channels; Ca^<2+>チャネル

To examine roles for presynaptic protein-protein interactions in neurotransmitter release, synapses formed between rat superior cervical ganglion neurons in culture were employed. The synaptic protein interaction site of several presynaptic proteins were synthesized and introduced into the presynaptic neurons through a glass patch pipette, while measuring synaptic transmission evoked by presynaptic action potentials. The change in postsynaptic responses, we speculated the function of the proteins as follows.1)CaィイD12+ィエD1-binding protein(A)synaptotagmin : trigger of synaptic vesicle fusion, promotion of endocytosis.(B)Doc2α-Munc 13 interacion : synaptic vesicle translocation to the release site.2)SNARE-associating proteins(A)N-type CaィイD12+ィエD1 channels : involved in synchronous transmitter release, voltage-dependent enhancement of transmitter release.(B)P/Q-type CaィイD12+ィエD1 channels : involved in neurotransmitter release.cSnapin : modulation of synaptotagmin-SNARE complex interaction.(D)Syntaphilin : control of SNARE complex assembly by syntaxin-1 clamping.

为了检查神经递质释放中突触前蛋白质相互作用的作用，采用了培养物中大鼠超颈神经节神经元之间形成的突触。合成了几种突触前蛋白的突触蛋白相互作用位点，并通过玻璃斑块移液管将其引入突触前神经元，同时测量突触前作用电位引起的合成传递。突触后反应的变化，我们猜测蛋白质的功能如下。1）CAI D12+IYE D1结合蛋白（A）突触tagmin：突触囊泡融合的触发器，内吞作用的触发，促进内吞作用。 proteins(A)N-type Cai D12+iye D1 channels: involved in synchronous transmitter release, voltage-dependent enhancement of transmitter release.(B)P/Q-type Cai D12+iye D1 channels : involved in neurotransmitter release.cSnapin: modulation of synaptotagmin-SNARE complex interaction.(D)Syntaphilin: control通过Syntaxin-1夹紧圈的圈套复合物组件。

项目成果

Mikoshiba, K., Fukuda, M., Niinobe, M., Mochida, S., Sugimori, M., Llinas, R.,: "Role of inositol polyphosphate in exocytosis."In The adrenal Chromaffin Cell. KANNO,T., NAKAZATO,Y., KUMAKURA,K, Eds. Hokkaido University Press.. 213-220 (1998)

Mikoshiba, K.、Fukuda, M.、Niinobe, M.、Mochida, S.、Sugimori, M.、Llinas, R.，：“肌醇多磷酸在胞吐作用中的作用”。在肾上腺嗜铬细胞中。

Mochida,S.: "Studies of neurotransmitter release at cholinergic synapses formed in culture"In Slow Synaptic : Responses and Modulation KUBA,K., HIGASHIDA,H., BROWN,D.A., YOSHIOKA,T., Eds. Springer-Verlag, Tokyo.. 235-246 (1999)

Mochida，S.：“在培养物中形成的胆碱能突触处神经递质释放的研究”，慢突触：反应和调节 KUBA，K.，HIGASHIDA，H.，BROWN，D.A.，YOSHIOKA，T.，编辑。

Sumiko Mochida: "Cellular and molecular mechanisms of toxin action: Secretory system"M. Linial and A. Grasso. 476(26) (1998)

Sumiko Mochida：“毒素作用的细胞和分子机制：分泌系统”M。

Mochida,S.: "Protein-protein interactions in neurotransmitter release."Neurosci. Res.. 36(3). 175-182 (2000)

Mochida，S.：“神经递质释放中的蛋白质-蛋白质相互作用。”神经科学。

Sumiko Mochida: "Role of the Doc2α-Muncl3-1 interactions in the neurotransmitter release process." Proc.Nath.Acad.Sci.USA. 95. 11418-11422 (1998)

Sumiko Mochida：“Doc2α-Muncl3-1 相互作用在神经递质释放过程中的作用。”Proc.Nath.Acad.Sci.USA。95. 11418-11422 (1998)