Molecular analysis of Genetic Polymorphisms in Blood and Development of Primers for Genotyping

血液中遗传多态性的分子分析和基因分型引物的开发

• 批准号: 09557038
• 负责人: YUASA Isao
• 金额: $ 7.62万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557038/
• 关键词: Red cell antigen types; Serum protein types; Red cell enzyme types; Nucleotide sequences; DNA polymorphisms; Primers; Paternity tests; 塩基配列

In this projects we have investigated the molecular bases of classical genetic markers in blood to propose simple and rapid genotyping methods. We analyzed (1)ABO, RH and Diego blood group systems as red cell antigens, (2)orosomucoid (alpha-1-acid glycoprotein), group-specific component, inter-alpha-trypsin inhibitor, the R subcomponent of the first component of component, the seventh component of complement, alpha-1-antitrypsin, and the B unit of coagulation factor XIII as serum proteins (3)phosphoglucomutase 1, eaterase D, DNase 1, acetaldehyde dehydrogenase 2, and phosphomannomutase 2 as red cell and other cell enzymes. We also analyzed some null alleles (ORM1ィイD1*ィエD1Q0koln and ITIH1ィイD1*ィエD1Q0iwate), which could cause a false incompatibility between father and child. A nucleotide substitution of de novo mutation, observed in the group-specific component in a German paternity case, was determined. The findings obtained in this study will be useful not only in forensic science but also biological and medical sciences.

在这个项目中，我们研究了血液中经典遗传标记的分子基础，提出了简单快速的基因分型方法，我们分析了 (1)ABO、RH 和 Diego 血型系统作为红细胞抗原，(2)orosumucoid (α-1-)。酸性糖蛋白）、组特异性成分、间α-胰蛋白酶抑制剂、成分第一成分的R亚成分、补体第七成分、α1-抗胰蛋白酶、以及B单位凝血因子 XIII 作为血清蛋白 (3) 磷酸葡萄糖变位酶 1、酯酶 D、DNase 1、乙醛脱氢酶 2 和磷酸甘露糖变位酶 2 作为红细胞和其他细胞酶），这可能会导致假性不相容。在德国亲子鉴定案例中观察到的群体特异性成分中存在从头突变的核苷酸替换，这项研究获得的结果不仅适用于法医学，而且适用于生物和医学科学。

项目成果

Watanabe G: "DXSIOOII; a lyper vavioffe tetra nucbtide STR polymorphism on the Xtbrcmosome"International Journal of Legal Meicine. (印刷中).

Watanabe G：“DXSIOOII；Xtbrcmosome 上的 lyper vavioffe 四核苷酸 STR 多态性”国际法律医学杂志（正在出版）。

Watanabe G: "Improved naplotype analysis of humcm myelin basic prptein (MBP)STR Coci"Human Biology. (印刷中).

Watanabe G：“改进的 humcm 髓磷脂碱性蛋白 (MBP)STR Coci 的单倍型分析”人类生物学（正在出版）。

K.Ago, I.Yuasa, K.Umetsu, O.Tsuganezawa: "Inter-alpha-trypsin inhibitor polymorphism in Southwestern Japan : Geographical clines allele frequencies in Japanese populations. Progress in Forensic Haemogenetics (G. F. Sensabaugh, B. Brinkmann, and P. J. Linc

K.Ago、I.Yuasa、K.Umetsu、O.Tsuganezawa：“日本西南部的α-胰蛋白酶抑制剂间多态性：日本人群中的地理 Clines 等位基因频率。法医血液遗传学进展（G. F. Sensabaugh、B. Brinkmann 和 P. J.

Weidinger, S.: "Detection of a de novo mutation in the GC system by DNA sequence analysis"Progress in Forensic Haemogenetics. 7. 240-242 (1998)

Weidinger, S.：“通过 DNA 序列分析检测 GC 系统中的从头突变”法医血液遗传学进展。

G.Watanabe, K.Umetsu, I.Yuasa, T.Suzuki: "DXS10011 : a hypervariable tetranucleotide STR polymorphism on the X chromosome"International Journal of Legal Medicine. (in press).

G.Watanabe、K.Umetsu、I.Yuasa、T.Suzuki：“DXS10011：X 染色体上的高变四核苷酸 STR 多态性”国际法律医学杂志。