Analysis of species specificity in endoparasitoids

内寄生蜂的物种特异性分析

• 批准号: 09660047
• 负责人: TANAKA Toshiharu
• 金额: $ 1.66万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660047/
• 关键词: host specificity; endoparasitoid; stage specificity; Cotesia kariyai; Cotesia ruficrus; Pseudaletia separata; Pseudaletia separata; カリヤコマユバチ; イネヨトウコマユバチ; ポリドナウイルス

Larval endoparasitoids have to regulate the host in the physiological condition to acquire the suitable state for the development of their eggs and larvae. Between two different parasitoids being able to attack the same host species (Pseudaletia separata), one parasitoid, Cotesia ruficrus is restricted to parasitize the young stadium of the host, while another parasitoid, Cotesia kariyai is able to parasitize successfully through all stadium. Reciprocal exchanging of the polydnavirus and the venom of each parasitoid produced the host regulation according to the characteristics of the polydnavirus. Each genomic DNA segments hybridized with the reciprocal genornic DNA segments of PDV.Furthermore, we reported the cloning and characterization of Cotesia kariyai polydnavirus (CkPV) genes expressed in host Pseudaletia separata testes. We isolated clones encoding CkPV mRNAs from a cDNA library of host testes. We found two motif sequences of the RNase T_2 family in one of CkPV gene expressed in host testes. The CkPV RNase T_2 fragment was hybridized to more than one viral genome segments. By northern blot analysis using cloned CkPV RNase T_2 gene as a probe, we showed the 3.1 kbp and 2.3 kbp mRNAs were detected 4-12h after parasitization, which Was before the appearance of cells with abnormal chromatin in the host testes.

内寄生蜂幼虫必须调节寄主的生理状态，以获得适合其卵和幼虫发育的状态。在能够攻击同一寄主物种（Pseudaletia separata）的两种不同寄生蜂之间，一种寄生蜂 Cotesia ruficrus 仅限于寄生于寄主的幼年体育场，而另一种寄生蜂 Cotesia kariyai 则能够成功寄生于所有寄主体育场。多DNA病毒与各寄生蜂毒液的相互交换产生了根据多DNA病毒的特性的宿主调节。每个基因组 DNA 片段与 PDV 的相应基因组 DNA 片段杂交。此外，我们报道了在宿主分离假睾丸中表达的 Cotesia kariyai 多 DNA 病毒 (CkPV) 基因的克隆和表征。我们从宿主睾丸的 cDNA 文库中分离出编码 CkPV mRNA 的克隆。我们在宿主睾丸中表达的 CkPV 基因之一中发现了 RNase T_2 家族的两个基序序列。 CkPV RNase T_2 片段与多个病毒基因组片段杂交。以克隆的CkPV RNase T_2基因为探针进行Northern印迹分析，我们发现在寄生后4-12小时，即在宿主睾丸中出现染色质异常细胞之前，检测到了3.1 kbp和2.3 kbp mRNA。

项目成果

田中 利治: "ポリドナウイルス-幼虫寄生バチの共生者" 植物防疫. 52. 499-504 (1998)

Toshiharu Tanaka：“多DNA病毒 - 幼虫寄生黄蜂的共生体”植物保护 52. 499-504 (1998)。

田中 利治: "ポリドナウイルス一幼虫寄生バチの共生者" 植物防疫. 52. 499-504 (1998)

Toshiharu Tanaka：“多DNA病毒 - 幼虫寄生蜂的共生体”植物保护 52. 499-504 (1998)。

Toshiharu Tanaka: "Polydnavirus-symbiont of larval endoparasitoid" Shokubutu-Boeki. 52(in Japanese). 499-504 (1998)

Toshiharu Tanaka：“多DNA病毒-内寄生幼虫的共生体”Shokubutu-Boeki。