Effects of Excimer Laser Radiation on Ocular Tissues

准分子激光辐射对眼组织的影响

• 批准号: 09671816
• 负责人: NAKAYAMA Kiyoo
• 金额: $ 2.05万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671816/
• 关键词: Excimer laser; Keratectomy; Subepithelial opacity; Descemet's membrane; Extracellular matrix; immunohistochemistry; PCR; corneal wound healing; LASIK; VI型コラーゲン; アスコルビン酸; 角膜創傷治癒; コラーゲン; プロテオグリカン; 長期的観察; 生物活性物質

1. Excimer laser keratectomy in rabbit(1) A number of keratocytes which had changed into fibroblastic cells, invaded into the subepithelial area of the ablated cornea. In this area, type III and IV collagens, chondroitin-G-sulfate and large proteoglycan were localized.(2) Abnormal deposit appeared in Descemets membrane at third day after excimer laser keratectomy and was remained until at least one year after the operation. Immuniohistochemical studies and RT-PCR method revealed that type VI collagen was contained in this deposit.(3) The healing process of the epithelial brasement membran and subepithelial stroma was still incomplete three years after excimer laser keratectomy.(4) Excimer laser radiation induced the increase of ascorbic acid in aquaous human 15 minutes after the operation. The clinical meaning of the result was unknown.2. Mechanical (Conventional) keratectomy in rabbitThe results previously mentioned on 1. (1)(2)(3) were also obtained in the rabbit corneas which received mechanical keratectomy. It seemed that the nonspecific process of wound healing occurred in rabbit cornea which received excimer laser keratectomy.3. LASIK (laser in situ keratomileusis)The damage to rabbit corneas induced by LASIK was mild to moderate. However it was very anxious that the wound healing after LASIK was very weak.

1.家兔准分子激光角膜切削术 (1)大量角膜细胞侵入角膜上皮下层，转变为成纤维细胞。在该区域，定位有III型和IV型胶原、G-硫酸软骨素和大蛋白多糖。(2)准分子激光角膜切除术后第三天后弹力层出现异常沉积，并持续到术后至少一年。免疫组织化学研究和RT-PCR方法显示该沉积物中含有VI型胶原。(3)准分子激光角膜切除术三年后，上皮支撑膜和上皮下基质的愈合过程仍然不完全。(4)准分子激光辐射诱发手术后15分钟，人水中的抗坏血酸增加。结果临床意义不明。2．兔子机械（传统）角膜切除术先前在 1.(1)(2)(3) 中提到的结果也在接受机械角膜切除术的兔子角膜中获得。准分子激光角膜切削术兔角膜出现非特异性伤口愈合过程。 3． LASIK（激光原位角膜磨镶术） LASIK对兔角膜造成的损伤为轻至中度。但令人非常担心的是，LASIK术后的伤口愈合很弱。

项目成果

Hotta Y. Fujiki K. Ono K. Fujimaki T. Nakayasu K. Yamaguchi T. Kanai A: "Arg124Cys mutation of the βig-h3 gene in a Japanese family with lattice corneal dystrophy type I"Jpn. J. Ophthalmol. 42. 450-455 (1998)

Hotta Y. Fujiki K. Ono K. Fujimaki T. Nakayasu K. Yamaguchi T. Kanai A：“患有 I 型角膜营养不良的日本家族中 βig-h3 基因的 Arg124Cys 突变”Jpn. 42。 -455 (1998)

久保慶和、有村秋子、渡部保男、中安清夫、金井淳: "ビタミンA欠乏色飼育家兎に対するビタミンAパルミテートの点眼効果"日眼会誌. 103(10). 729-733 (1999)

Yoshikazu Kubo、Akiko Arimura、Yasuo Watanabe、Kiyoo Nakayasu、Jun Kanai：“维生素 A 棕榈酸酯滴眼液对维生素 A 缺乏的有色兔子的功效”，日本眼科协会杂志 103(10)。

久保慶和、有村秋子、渡部保男、中安清夫、金井淳: "ビタミンA欠乏食飼育家兎に対するビタミンAパルミテートの点眼効果"日眼会誌. 103(10). 729-733 (1999)

Yoshikazu Kubo、Akiko Arimura、Yasuo Watanabe、Kiyoo Nakayasu、Jun Kanai：“维生素 A 棕榈酸酯滴眼液对缺乏维生素 A 饮食的兔子的功效”日本眼科协会杂志 103(10)。 ）

加藤卓次、中安清夫: "Eye Surgery Now Illustrated No.6「水疱性角膜症」大橋裕一編"メディカルビュー社. (124-129)6 (1997)

加藤卓司、中安清雄：“眼科手术图解第 6 期“泡状角膜病”，大桥雄一编辑”Medical View Publishing（124-129）6（1997 年）。

Kato T, Nakayasu K, Ikegami K, Obara T, Kanayama T, Kanai A.: "Analysis of glycosaminoglycans in rabbit cornea after excimer laser keratectomy"Br J. Ophthalmol. 83. 609-612

Kato T、Nakayasu K、Ikegami K、Obara T、Kanayama T、Kanai A.：“准分子激光角膜切除术后兔角膜中糖胺聚糖的分析”Br J. Ophamol。