The effect of anesthetics and anesthetic metbolite on mesangial cells proliferation.

麻醉剂和麻醉代谢物对系膜细胞增殖的影响。

• 批准号: 09671596
• 负责人: SATA Takeyoshi
• 金额: $ 0.77万
• 依托单位: University of Occupational Environmental Health(UOEH)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671596/
• 关键词: mesangial cell; ketamine; propofol; [Protein Kinase A; Protein Kinase; 蛋白燐酸化酵素; 〔^3H〕-thymidine; 尿中代謝産物; メサンギウム細胞; ケタミン; protein kinase A; protein kinase C

We studied the effects of intravenous anesthetics ketamine and propofol on rat mesangial cell (MC) proliferation. Ketamine inhibited [3H]tymidine incorporation into the mesangial cells in a concentration-dependent manner (10 mM*1 mM). But, Propofol had no effects on [3H]tymidine incorporation. Ketamine also attenuated the cell number at clinical concentrations and increased cAMP levels in MC.Protein Kinase A inhibitor recovered the inhibition of ketamine on MC proliferation. Ketamine kept the renal blood flow in Rat in vivo. These results suggested that ketamine have inhibitory effects on the MC proliferation via protein kinase pathway and ketamine may be useful to suppress the progression of chronic renal failure.

我们研究了静脉麻醉药氯胺酮和丙泊酚对大鼠肾小球细胞（MC）增殖的影响。氯胺酮以浓度依赖性方式（10 mm*1 mm）抑制[3H]开甲胺掺入肾小球细胞中。但是，丙泊酚对[3H]开甲胺的掺入没有影响。氯胺酮还以临床浓度减弱了细胞数，MC中的cAMP水平升高。蛋白激酶A抑制剂回收了氯胺酮对MC增殖的抑制作用。氯胺酮在大鼠体内保持肾血流。这些结果表明，氯胺酮通过蛋白激酶途径对MC增殖具有抑制作用，氯胺酮可能有助于抑制慢性肾衰竭的进展。

项目成果

Minami K., et al.: "Inhibitory effect of anesthetics and ethanol on muscarinic receptors expressed in xenopus oocytes." European Journal of Pharmacology. 339. 237-244 (1997)

Minami K. 等人：“麻醉剂和乙醇对爪蟾卵母细胞中表达的毒蕈碱受体的抑制作用。”

Jimi N et.al: "Inhibitory eddects of the intravenous anesthetic,ketamine,on rat mesangial cell proliferation" Anesthesia and Analgesia. 84. 190-195 (1997)

Jimi N 等人：“静脉麻醉剂氯胺酮对大鼠系膜细胞增殖的抑制作用”麻醉和镇痛。

Segawa K et.al: "The inhibitory effect of adrenomedullin on rat mesangial cell mitogenesis." Nephron. 74. 577-579 (1996)

Sekawa K 等人：“肾上腺髓质素对大鼠系膜细胞有丝分裂的抑制作用。”