The establishment of the method which detects the minimal residual leukemic cells in acute myelogenous patients in remisson and predicts the patients prognosis.

建立了急性髓系缓解期患者微量残留白血病细胞检测及预测患者预后的方法。

• 批准号: 09672352
• 负责人: NARA Nobuo
• 金额: $ 2.05万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672352/
• 关键词: acute myelogenous leukemia; leukemic cells; leukemic balst progenitors; cytokines; WT-1 gene; 急性白血病; 白血病コロニー; 免疫グロブリン重鎖遺伝子; T細胞レセプター遺伝子

Acute myelogenous leukemia (AML) is highly lethal malignant hemopathy. To cure AML patients, malignant leukemic cells should be completely purged from the patients. Most of AML can achieve complete remission by intensive chemotherapy and/or bone marrow transplantation. Some of them, however, suffer from the relapse of AML.The major issue in leukemia therapy is how to detect the residual leukemic cells in patients and how to eradicate such residual leukemic cells.To predict the clinical outcome of AML patients, we have studied the biological properties of leukemic blast progenitors and the gene expression of WT-1 mRNA.The proliferation of leukemic cells is supported by a small subpopulation of leukemic blast progenitors with self-renewal capacity. The self-renewal capacity has been shown to significantly correlate with the patients prognosis. WT-1 gene was expressed in 22 of 24 patients studied. The expression level of WT-l mRNA, however, was not correlated with either the proliferation activity of leukemic or clinical outcome of AML patients.Further studies are necessary to find any marker that can highly predict the clinical prognosis of AML patients and to establish optimal treatment protocol tailored for each AML patient.

急性骨髓性白血病（AML）是高致命的恶性肿瘤。为了治愈AML患者，应从患者中完全清除恶性白血病细胞。大多数AML可以通过强化化疗和/或骨髓移植完全缓解。然而，其中一些人患有AML的复发。白血病疗法的主要问题是如何检测患者的残留白血病细胞，以及如何消除这种残留的白血病细胞。为了预测AML患者的临床结果，我们已经研究了白血病爆炸的生物学特性，由wt-1 mrna的plimifififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififififif。具有自我更新能力的爆炸祖细胞。自我更新能力已显示与患者预后显着相关。在研究的24名患者中，有22名表达了WT-1基因。然而，WT-L mRNA的表达水平与AML患者的白血病或临床结局的增殖活性无关。FURTHER研究对于找到可以高度预测AML患者的临床预后的任何标记是必要的，以建立针对每位AML患者量身定制的最佳治疗方案。

项目成果

Tohda S,Maruyama M,and Nara N: "Molecular typing of methicillin-resistant staphylococcus aureus by polymerase chain reaction: distribution of the typed strains in hospitals." Internal Medicine. 36-10. 694-699 (1997)

Tohda S、Maruyama M 和 Nara N：“通过聚合酶链反应对耐甲氧西林金黄色葡萄球菌进行分子分型：分型菌株在医院中的分布。”

Nara N, Kurokawa H, Tohda S, Tomiyama J, Nagata K, Tanikawa S: "Effect of vesnarinone,a quinolinone derivative,on the growth of leukemic blasts in acute myelogenous leukemia." Experimental Hematology. 25・3. 199-204 (1997)

Nara N、Kurokawa H、Tohda S、Tomiyama J、Nagata K、Tanikawa S：“喹啉酮衍生物 vesnarinone 对急性髓性白血病中白血病细胞生长的影响 25・3。” 1997）

Saito K,Nakamura Y,Waga K,Hirota K,Inoue F,Enokihara H,Nara N and Furusawa S: "Mature and immature myeloid cells decrease the granulocyte colony-stimulating factor level by absorption of granulocyte colony-stimulating factor." International Journal of Hem

Saito K、Nakamura Y、Waga K、Hirota K、Inoue F、Enokihara H、Nara N 和 Furusawa S：“成熟和未成熟的骨髓细胞通过吸收粒细胞集落刺激因子来降低粒细胞集落刺激因子水平。”

Saito K, Nakamura Y, Waga K, Hirota K, Inoue F, Enokihara H, Nara N and Furusawa S: "Mature and immature myeloid cells decrease the granulocyte colony-stimulating factor level by absorption of granulocyte colony-stimulating factor." International Journal

Saito K、Nakamura Y、Waga K、Hirota K、Inoue F、Enokihara H、Nara N 和 Furusawa S：“成熟和未成熟的骨髓细胞通过吸收粒细胞集落刺激因子来降低粒细胞集落刺激因子水平。”

Hamaguchi H,Inokuti K,Nara N,Nagata K,Yamamoto K,Yagasaki F,Dan K: "Alterations in the colorectal carcinoma gene and protein in a n〓 human myeloid leukemia cell line with trisomy 18 established f〓 overt leukemia after myelodysplastic syndrome." Internatio

Hamaguchi H、Inokuti K、Nara N、Nagata K、Yamamoto K、Yagasaki F、Dan K：“具有 18 三体性的人骨髓性白血病细胞系中结直肠癌基因和蛋白质的改变在骨髓增生异常综合征后确立了明显的白血病。”Internatio