Search for a Drosophila retinoid-binding protein and its functional analysis.

寻找果蝇类视黄醇结合蛋白及其功能分析。

基本信息

批准号：
09680689
负责人：
SUZUKI Emiko
金额：
$ 0.77万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680689/
关键词：
Drosophila retinoid vision vitamin A photoreception signal transduction Drosophila 複眼突然変異レチナ-ル

项目摘要

1. Search for a Drosophila homologue of vertebrate retinoid binding proteins by screening of cDNA library : We have hot detect a homologue of vertebrate retinoid binding proteins during the term of this project. This result was compatible with the data from the Drosophila genome project which became open for public at the end of this project.2. Analysis of the Drosophila homologue of squid retinaldehyde binding protein in the Drosophila retina : We found a protein of 120kDa that is immunoreactive for anti-squid retinaldehyde protein, in Drosophila compound eyes and ocelli. This protein localized in the interphotoreceptor matrix and appeared to be Drosophila retinaldehyde binding protein. The possibility that the ninaD gene, whose mutation affects retinal metabolism, encodes this protein is flow under investigation.3. Search for a Drosophila homologue of vertebrate RPE65 : RPE65 is a protein specifically expressed in the retinal pigment epithelium in vertebrates. The primary structure of this protein is extremely well conserved, and thought to be an important protein for the visual cycle. As no invertebrate homologue of RPE65 was known at the beginning of this project, we screened the Drosophila homologue of this protein. We found a protein (DRPE65) which has about 40% amino acid identity with vertebrate RPE65. The analysis of intramolecular motifs revealed that similar motifs exist not only lower invertebrates but also plants. These results suggest that the RPE65 related protein constitute a protein family involved in retinoid and carotenoid metabolism.4. Functional analysis a phototransduction-related protein INAD : We have analyzed the function of phototransduction-related protein INAD, which is tightly related with the metabolism of visua substance. We found that the INAD protein is essential for the phototransduction by tethering several proteins involved in this process.

1。通过筛选cDNA库来搜索脊椎动物类维生素类似结合蛋白的果蝇同源物：在本项目期间，我们有热检测脊椎动物类维生素类似结合蛋白的同源物。该结果与果蝇基因组项目的数据兼容，该项目在该项目结束时向公众开放。2。分析果蝇视网膜中鱿鱼视网膜甲醛结合蛋白的果蝇同源物：我们发现一种120KDA的蛋白质，可免疫反应，可用于抗磷果实的抗磷酸视网膜葡萄酒蛋白，果蝇化合物和ocelli。该蛋白质定位于photoreceptor基质中，似乎是果蝇视网膜甲醛结合蛋白。 Ninad基因突变会影响视网膜代谢的可能性，该蛋白质正在研究中。3。搜索脊椎动物RPE65：RPE65的果蝇同源物是一种在脊椎动物的视网膜色素上皮中特异性表达的蛋白质。该蛋白质的主要结构非常保守，被认为是视觉周期的重要蛋白质。由于该项目的开头尚不知道RPE65的无脊椎动物同源物，因此我们筛选了该蛋白质的果蝇同源物。我们发现了一种蛋白质（DRPE65），该蛋白质与脊椎动物RPE65具有约40％的氨基酸身份。分子内基序的分析表明，相似的基序不仅存在较低的无脊椎动物，而且还存在植物。这些结果表明，RPE65相关的蛋白质构成了与类维生素类似和类胡萝卜素代谢有关的蛋白质家族。4。功能分析一种与光转导相关的蛋白质INAD：我们分析了光转传相关蛋白INAD的功能，这与Visua物质的代谢密切相关。我们发现，INAD蛋白通过绑定参与此过程的几种蛋白质而对光转移至关重要。