Functional analysis of the diacylglycerol kinase in Drosophila photoreceptor cells

果蝇感光细胞二酰甘油激酶的功能分析

• 批准号: 12680685
• 负责人: SUZUKI Emiko
• 金额: $ 2.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680685/
• 关键词: Drosophila; diacylglycerol kinase; visual mutant; retial degeneration; signal transduciton; phototransduciton; immunocyrochemistry; electron microscopy; 免疫電子顕微鏡法; 視細胞; 網膜変成; 光情報交換; システインリッチドメイン; 免疫組織化学

The final goal of this research project is to elucidate the mechanism for the functional expression of the diacylglycerol kinase (RDGA/DAGK2) that is a key enzyme of the phototransduction in Drosophila photoreceptors. Our previous studies by immnoelectron microscopy of photoreceptor cells revealed that RDGA protein localizes to the subcellular region at the bases of photoreceptive microvilli, subrhabdomeric region in which a specialized network of smooth endoplasmic reticulum resides. In the present study, we studied the domain functions of RDGA protein by the expression studies on the modified RDGA proteins. Our results demonstrated that the cysteine-rich domains (CRDs) of the RDGA protein are essential for the localization and enzyme activity of the protein. On the other hand, the ankyrin repeats at the C-terminus was unnecessary for the primary function of RDGA protein, suggesting that they have other functions. This remains to be uncovered by the future studies.

该研究项目的最终目标是阐明二酰基甘油激酶（RDGA/DAGK2）功能表达的机制，该机制是果蝇光感受器中光转移的关键酶。我们先前对光感受器细胞的Immnoelectron显微镜的研究表明，RDGA蛋白位于光感受器微绒毛下的亚养蜂区域的亚细胞区域，该区域是光滑内质网的专业网络，其中居住在该区域。在本研究中，我们通过改良的RDGA蛋白的表达研究研究了RDGA蛋白的域功能。我们的结果表明，RDGA蛋白的富含半胱氨酸的结构域（CRD）对于蛋白质的定位和酶活性至关重要。另一方面，C末端重复的Ankyrin对于RDGA蛋白的主要功能不必要，这表明它们具有其他功能。未来的研究仍然有待发现这一点。

项目成果

Suzuki, E: "High-resolution scanning electron microscopy of immunogold-labelled cells by the use of thin plasma coating of osmium"Journal of Neuroscience Research. 21. 150-158 (2001)

Suzuki, E：“使用锇等离子薄层对免疫金标记细胞进行高分辨率扫描电子显微镜观察”神经科学研究杂志。

Kaneko, A. (ed.): "The Neural Basis of Early Vision"Springer-Verlag. 253 (2003)

Kaneko, A.（编辑）：“早期视觉的神经基础”施普林格出版社。

E.Suzuki: "High-resolution scanning electron microscopy of immunogold-labelled cells by the use of thin plasma coating of osmium"J. Microscopy. 208. 153-157 (2002)

E.Suzuki：“使用锇等离子薄层对免疫金标记细胞进行高分辨率扫描电子显微镜观察”J。

Tsunoda, S.: "Independent anchoring and assembly mechanisms of INAD signaling complexes in Drosophila photoreceptors"Journal of Neuroscience. 21. 150-158 (2001)

Tsunoda, S.：“果蝇光感受器中 INAD 信号复合物的独立锚定和组装机制”神经科学杂志。

Kaneko, A. (Ed.): "The Neuroal Basis of Early Vision"Springer-Verlag. 253 (2003)

Kaneko, A.（主编）：“早期视觉的神经基础”施普林格出版社。