Sperm Receptor of Bovine Egg Surface Zona Pellucida

牛卵子表面透明带的精子受体

基本信息

批准号：
09680687
负责人：
NAKANO Minoru
金额：
$ 2.37万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

(1997)1 : Bovine sperm receptor was shown to be involved In the carbohydrate moieties of zona glyciproteins by an in vitro competition assay. 2 : Competition assay also revealed that bovine sperm mainly bind to a high-mannnose type carbohydrate chain, Man_5GlcNAc_2 which is major neutral N-linked chain found in bovine zona proteins. It has been reported that mouse sperm bind to beta-galactosyl residues at the non-reducing ends of O-linked chain of ZP3 protein, while porcine sperm to beta-galactosyl residues at the non-reducing ends of N-linked chains of ZPB.Our results on the bovine sperm receptors contribute much to the elucidation of the mechanism of species-specific sperm-egg binding. 3 : In order to obtain O-linked chains, the zona proteins were treated with alkali under various conditions. The activities, however, could not be measured, since N-linked chains were also cleaved partly together with O-linked chains under any conditions.(1998)1 : The receptor active carbohydrate chain was shown to be present in ZPB protein. It is unclear whether the active chain is present in ZPA, since heteropolymers were shown to be contaminated in ZPA obtained by HPLC, We are trying to purify ZPA.2 : Large scale preparation of ZPB for determination of the localization of receptor active carbohydrate chain in ZPB is also In progress. 3 : It was shown that the receptor active chain, Man_5GlcNAc_2, inhibits bovine in vitro fertilization (IVF) and its derivatives Man_3GlcNAc_2, ManG1cNAc_2, and alpha-methylmannoside also weakly inhibit IVF, indicating the essential role of mannose residue(s) at the non-reducing ends in bovine IVF.4 : Treatment of the unfertilized eggs with sialidase reduced the number of binding sperm to the eggs to about 50%, suggesting that sialic acids also play a role in the bovine sperm binding to the eggs.

（1997）1：通过体外竞争测定法显示牛精子受体与Zona糖蛋白的碳水化合物部分有关。 2：竞争测定法还显示，牛精子主要与高甘露糖型碳水化合物链结合，MAN_5GLCNAC_2，这是在牛Zona蛋白中发现的主要中性N连接链。 It has been reported that mouse sperm bind to beta-galactosyl residues at the non-reducing ends of O-linked chain of ZP3 protein, while porcine sperm to beta-galactosyl residues at the non-reducing ends of N-linked chains of ZPB.Our results on the bovine sperm receptors contribute much to the elucidation of the mechanism of species-specific sperm-egg binding. 3：为了获得O连锁链，在各种条件下用碱处理Zona蛋白。然而，由于在任何条件下，N连锁链也部分与O连锁链一起裂解，因此无法测量这些活性。（1998）1：受体活性碳水化合物链被证明存在于ZPB蛋白中。目前尚不清楚是否存在ZPA中的活性链，因为在HPLC获得的ZPA中显示了杂聚物，我们正在尝试净化ZPA.2：ZPB的大规模制备以确定受体活性碳水化合物在ZPB中的定位。 3 : It was shown that the receptor active chain, Man_5GlcNAc_2, inhibits bovine in vitro fertilization (IVF) and its derivatives Man_3GlcNAc_2, ManG1cNAc_2, and alpha-methylmannoside also weakly inhibit IVF, indicating the essential role of mannose residue(s) at the non-reducing ends in bovine IVF.4 :用唾液酸酶处理未施肥的卵将与卵的结合精子数量降低到约50％，这表明唾液酸在牛与卵的结合中也起作用。