Structure and Function of Pig and Bovine Egg Zona Pellucida

猪和牛卵透明带的结构和功能

• 批准号: 06680676
• 负责人: NAKANO Minoru
• 金额: $ 1.41万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680676/
• 关键词: Zona Pellucida; Mammalian Egg; Fertilization; Glycoprotein; Cell Recognition; Sperm-Egg Interaction; Sperm Receptor; 哺乳類; 卵子; 細胞接着

1.1994(1) A glycoprotein component of pig zona pellucida, PZP3alpha, has sperm receptor activity. O-glycoside chain eliminated PZP3alpha retained the activity, while N-glycoside chain eliminated PZP3alpha lost the activity, indicating that boar sperm bind to the N-linked chain (s).(2) Tri-or tetra-antennnary N-linked chain was shown to be the pig sperm receptor.(3) The high mannose-type N-linked chains, which are absent from pig and mouse zona proteins, have been found in the bovine zona proteins.(4) The sperm receptor activity of the N-linked chains isolated from bovine zona proteins is shown to be quite low.(5) Three components of pig zona proteins showed cross-reactive antigenicity with respective bovine zona proteins.2.1995(1) Sialic acids of bovine zona proteins were shown to be eliminated during fertilization, resulting in neutralization of the acidic carbohydrate chains.(2) Bovine ZPA protein was shown to be cleaved specifically during fertilization.(3) Cysteine residues in bZPA component were shown to be oxidized during fertilization, inducing zona hardening.

1.1994（1）Pig Zona pellucida的糖蛋白成分Pzp3alpha具有精子受体活性。 O-glycoside chain eliminated PZP3alpha retained the activity, while N-glycoside chain eliminated PZP3alpha lost the activity, indicating that boar sperm bind to the N-linked chain (s).(2) Tri-or tetra-antennnary N-linked chain was shown to be the pig sperm receptor.(3) The high mannose-type N-linked chains, which are absent from pig and mouse zona proteins, have been found in the bovine zona proteins.(4) The sperm receptor activity of the N-linked chains isolated from bovine zona proteins is shown to be quite low.(5) Three components of pig zona proteins showed cross-reactive antigenicity with respective bovine zona proteins.2.1995(1) Sialic acids of bovine zona proteins were shown to be eliminated在受精过程中，导致酸性碳水化合物链中和。（2）牛ZPA蛋白在受精过程中被证明是特异性裂解的。（3）BZPA成分中的半胱氨酸残基被证明在受精过程中被氧化，从而诱导Zona耐药。

项目成果

Satoru Noguchi: "Characterization of the Zona Pellucida Glycoprotiein from Bovine Ovarian and Fertilized Eggs" Biochimica et Biophysica Acta. 1201. 7-14 (1994)

Satoru Noguchi：“牛卵巢和受精卵透明带糖蛋白的表征”Biochimica et Biophysicala Acta。

Naoto Yonezawa: "Binding of pig sperm receptor in the zona pellucida to the boar sperm acrozome" Journal of Reproduction and Fertility. 103. 1-8 (1994)

Naoto Yonezawa：“透明带中的猪精子受体与公猪精子顶体的结合”《生殖与生育杂志》。

N.Yonezawa, H.Aoki, Y.Hatanaka & M.Nakano.: "Involvement of N-linked carbohydrate chains of pig zona pellucida in sperm-egg binding." European Journal of Biochemistry. 233 (1). 35-41 (1995)

N.米泽、H.青木、Y.畑中

Makoto Yoshizawa: "Native zona pellucida structure is required for completion of sperm acrosome reaction in porcine fertilization" Theriogenology. 41. 1307-1313 (1994)

Makoto Yoshizawa：“猪受精过程中精子顶体反应的完成需要天然透明带结构”动物发生学。

K.Akama, M.Kosuge, H.Sato, Y.Yamaoka M.Nakano & T.Tobita: "Isolation of infact transition protein 1 and 3 from boar late spermatid nuclei." Biochemistry and Molecular Biology International. 32 (4). 615-622 (1994)

K.Akama、M.Kosuge、H.Sato、Y.Yamaoka M.Nakano