Structural basis of novel quadruplex-duplex switching of nucleic acids

新型核酸四链体-双链体转换的结构基础

• 批准号: 09680648
• 负责人: KATAHIRA Masato
• 金额: $ 2.11万
• 依托单位: Yokohama National University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680648/
• 关键词: DNA; RNA; NMR; quadruplex; duplex; telomere; protein; antitumor drug; 金属イオン; ドラッグ; リボザイム

The novel quadruplex and duplex structures of d(GGAGGA), formed in the presence and absence of potassium ions, respectively, were determined by NMR.It is notable that the duplex structure is composed exclusively of non-standard G : G and A : A basepairs.It was found for the first time that the RNA-binding protein, hnRNP D0 has the ability to transform the quadruplex structure to a single-stranded form. The structures and interactions with RNA of hnRNP DO and the related protein, Musashi1, were determined by NMR.It was suggested that this unique activity may be related to the maintenance of the telomere.The structure of the novel antitumor drug, UCH9, complexed with DNA was determined by NMR.The B-form to A-form structural transition caused by drug-binding was identified, and its biological significance was implied.

在存在和不存在钾离子的情况下形成的D（Ggagga）的新型四链体和双链结构，由NMR确定，值得注意的是，双工结构仅由非标准的G：G：G：G：G：G：G和A组成，由basep.it组成。单链形式。 The structures and interactions with RNA of hnRNP DO and the related protein, Musashi1, were determined by NMR.It was suggested that this unique activity may be related to the maintenance of the telomere.The structure of the novel antitumor drug, UCH9, complexed with DNA was determined by NMR.The B-form to A-form structural transition caused by drug-binding was identified, and its biological significance was implied.

项目成果

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Katahira 等人：“Musashi1 的 c 末端 RNA 结合结构域的结构主链动力学及其与 RNA 的相互作用”J. Mol.. 287. 314-330 (1999)