Two-photon two-color excited polarized fluorescence in biologically relevant molecules: investigation of molecular excited state structure and dynamics

生物相关分子中的双光子双色激发偏振荧光：分子激发态结构和动力学的研究

• 批准号: 71349453
• 负责人: Professor Dr. Karl-Heinz Gericke
• 金额: --
• 依托单位: Institut für Physikalische und Theoretische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71349453?language=en
• 关键词: Two; photon; two; color; excited

In the framework of a joint research project the alignment, orientation and rotational correlation time of biological relevant molecules will be studied. The molecules will be aligned and oriented using two color two photon (2c2p) laser pulses under different polarization schemes (here tunable radiation around 400nm and 800nm). The fluorescence is observed for different polarization angle as well. The advantage of using two different photons is twofold: shorter wavelengths (down to 265nm) can be reached making label-free proteine detection possible and different alignments of the molecules become possible compared to two photon excitation using identical photons. Due to diffusion processes, the initial aligned molecules will finally be distributed isotropically. We intend to determine the corresponding rotational correlation time which contains information on the interaction of the molecule with its surrounding. In a proof of principle PTP was investigated. The research work will be focused on the study of tryptophan, as characteristic chromophore for probing proteins, and indole, a molecule which shows some unusual spectroscopic features. In addition, some 2c2p experiments on indole have been performed by other groups. The results will be theoretically and experimentally supported by the research group of O. Vasyutinskii.

在联合研究项目的框架中，将研究对齐生物学相关分子的方向和旋转相关时间。分子将在不同的极化方案（在400nm和800nm左右的可调辐射方面）使用两种颜色两个光子（2C2P）激光脉冲对齐和定向。对于不同的极化角也观察到荧光。使用两个不同的光子的优点是双重的：可以达到较短的波长（向下至265nm），与使用相同的光子相比，与两个光子激发相比，可以实现无标签的蛋白检测，并且可以使用不同的分子比对。由于扩散过程，初始排列的分子最终将通过各向同性分布。我们打算确定相应的旋转相关时间，其中包含有关分子与周围环境相互作用的信息。为了证明PTP原则的证明。研究工作将集中在色氨酸的研究上，作为用于探测蛋白质的特征性发色团，而吲哚，吲哚是一种显示一些异常光谱特征的分子。此外，其他组已经进行了一些关于吲哚的2C2P实验。结果将由O. vasyutinskii的研究小组在理论上和实验上得到支持。