Analysis of polyamine regulation systems in plants and exploration of functional molecules based on polyamine structure

植物多胺调控系统分析及基于多胺结构的功能分子探索

• 批准号: 23780345
• 负责人: TAKAHASHI Yoshihiro
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780345/
• 关键词: 代謝工学; ポリアミン; ポリアミンオキシターゼ; イネ; 病原菌抵抗性反応; シロイヌナズナ

Polyamines are important not only for developmental processes but also for various environmental stress responses in plants. In order to elucidate the regulatory system responsible for polyamine homeostasis and action, I focused on polyamine degradationenzyme, polyamine oxidase (PAO), and identified three different genes coding for main PAOs in rice. These proteins were localized to peroxisomes and catalyzed polyamine back conversion. Moreover, it was found that one of the common tetraamines, thermospermine, is involved in disease resistance as a signal molecule like spermine.

多胺不仅对于发育过程很重要，而且对于植物的各种环境胁迫反应也很重要。为了阐明负责多胺稳态和作用的调节系统，我重点研究了多胺降解酶、多胺氧化酶（PAO），并确定了编码水稻中主要 PAO 的三个不同基因。这些蛋白质定位于过氧化物酶体并催化多胺反向转化。此外，人们还发现，常见的四胺之一——热精精胺，与精胺一样作为信号分子参与抗病性。

项目成果

Exogenous thermospermine has an activity to induce a subset of the defense genes and restrict cucumber mosaic virus multiplication in Arabidopsis thaliana.

外源性热精胺具有诱导拟南芥防御基因子集并限制黄瓜花叶病毒增殖的活性。

• DOI: 10.1007/s00299-012-1243-y
• 发表时间: 2012
• 影响因子: 6.2
• 作者: Sagor GHM; Takahashi H; Niitsu M; Takahashi Y; Berberich T; Kusano T
• 通讯作者: Kusano T

Constitutively and highly expressed Oryza sativa polyamine oxidases localize in peroxisomes and catalyze polyamine back conversion

稻多胺氧化酶组成型和高表达定位于过氧化物酶体并催化多胺反向转化

• DOI: 10.1007/s00726-011-1002-3
• 发表时间: 2012
• 影响因子: 3.5
• 作者: Ono; Y.; Kim; D.W.; Watanabe; K.; Sasaki; A.; Niitsu; M.; Berberich; T.; Kusano; T.;Takahashi; Y
• 通讯作者: Y