A study on epithelial-mesenchymal transition mediated by deltaNp63 in invasion and metastasis of oral squamous cell carcinoma

deltaNp63介导的上皮间质转化在口腔鳞癌侵袭转移中的研究

• 批准号: 23792358
• 负责人: KAWANO Shintaro
• 金额: $ 2.66万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792358/
• 关键词: 上皮間葉転換; 口腔扁平上皮癌; deltaNp63; 浸潤; 転移; 上皮-間葉転換; ΔNp63; 癌幹細胞; ⊿Np63; delta p63

In this study, we investigated the role of deltaNp63 in EMT during progression of oral squamous cell carcinoma (OSCC). The expressions of deltaNp63 and epithelial markers was detected in the OSCC cells, but not in SQUU-B cells (high metastatic potential). Mesenchymal markers were strongly expressed in the SQUU-B cells. Knockdown of endogenous deltaNp63 in HSC-2 cells induced morphological changes to the spindle shape, decreased the expression of epithelial markers, increased the expression of mesenchymal markers, increased migration. By contrast, SQUU-B cells overexpressing deltaNp63 showed changed their morphology from stromal cell-like to epithelial cells. Immunohistochemical staining revealed decreased intensity of deltaNp63 was positively associated with metastases and poor prognosis in the OSCC patients. These results indicated that deltaNp63 downregulation in cancer cells induces a mesenchymal phenotype that is related to tumor progression of OSCC.

在这项研究中，我们研究了 deltaNp63 在口腔鳞状细胞癌 (OSCC) 进展过程中 EMT 中的作用。在 OSCC 细胞中检测到 deltaNp63 和上皮标志物的表达，但在 SQUU-B 细胞（高转移潜能）中未检测到。间充质标志物在 SQUU-B 细胞中强烈表达。 HSC-2细胞中内源性deltaNp63的敲低引起纺锤形的形态改变，上皮标记物的表达减少，间质标记物的表达增加，迁移增加。相比之下，过表达 deltaNp63 的 SQUU-B 细胞显示其形态从基质细胞样改变为上皮细胞。免疫组织化学染色显示 deltaNp63 强度降低与 OSCC 患者的转移和不良预后呈正相关。这些结果表明，癌细胞中 deltaNp63 下调会诱导与 OSCC 肿瘤进展相关的间充质表型。

项目成果

Possible involvement ofΔNp63 downregulation in the invasion and metastasis of oral squamous cell carcinoma via induction of a mesenchymal phenotype

ΔNp63下调可能通过诱导间充质表型参与口腔鳞状细胞癌的侵袭和转移

• 发表时间: 2014
• 影响因子: 4
• 作者: Y. Goto; S. Kawano; R. Matsubara; T. Kiyosue; M. Hirano; T. Jinno; Y. Maruse; T. Toyoshima; R. Kitamura; H. Tanaka; K. Oobu; S. Nakamura
• 通讯作者: S. Nakamura

Clinical significance of combined assessment of the maximum standardized uptake value of F-18 FDG PET with nodal size in the diagnosis of cervical lymph node metastasis of oral squamous cell carcinoma

F-18 FDG PET最大标准化摄取值与淋巴结大小联合评估诊断口腔鳞癌颈部淋巴结转移的临床意义

• 发表时间: 2012
• 影响因子: 4.8
• 作者: Ryota Matsubara; Shintaro Kawano; Toru Chikui; Takahiro Kiyosue; Yuichi Goto; Mitsuhiro Hirano; Teppei Jinnno; Tetsuji Nagata; Kazunari Oobu; Koichiro Abe;Seiji Nakamura
• 通讯作者: Seiji Nakamura

口腔扁平上皮癌におけるinterleukin-6発現と臨床病理学的所見との関連

口腔鳞癌中IL-6表达与临床病理的关系

• 发表时间: 2013
• 作者: 神野哲平; 川野真太郎; 平野充広; 松原良太; 清末崇裕; 後藤雄一; 丸瀬靖之; 今藤彰子; 豊嶋健史; 田中秀明; 大部一成; 中村誠司
• 通讯作者: 中村誠司

Association of cytokeratin 17 expression with differentiation in oral squamous cell carcinoma

细胞角蛋白17表达与口腔鳞状细胞癌分化的关系

• 发表时间: 2012
• 作者: Ryoji Kitamura; Takeshi Toyoshima; Hideaki Tanaka; Shintaro Kawano; Kazunari Oobu; Takahiro Kiyosue; Ryota Matsubara; Yuichi Goto;Seiji Nakamura
• 通讯作者: Seiji Nakamura

口腔扁平上皮癌高転移株より分離した上皮-間葉転換細胞の特性

高转移性口腔鳞状细胞癌上皮间质转化细胞的特征

• 发表时间: 2013
• 作者: 平野充広; 川野真太郎; 松原良太; 清末崇裕; 後藤雄一; 神野哲平; 丸瀬靖之; 豊嶋健史; 北村亮二; 田中秀明; 大部一成; 中村誠司
• 通讯作者: 中村誠司