Plant genes required for arbuscular mycorrhiza symbiosis

丛枝菌根共生所需的植物基因

基本信息

批准号：
61396832
负责人：
Professor Dr. Martin Parniske
金额：
--
依托单位：
Institut für Genetik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2008
资助国家：
德国
起止时间：
2007-12-31 至 2011-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/61396832?language=en
关键词：
Plant genes required arbuscular mycorrhiza

项目摘要

The phosphate acquiring arbuscular mycorrhiza (AM) symbiosis between plant roots and Glomeromycota fungi is of prime ecological importance and bears potential for sustainable agriculture. We would like to unravel the molecular mechanisms that control development and regulation in this symbiosis. To this end, we have initiated a genetic screen for AM-defective individuals within an ethylmethane sulfonate (EMS)-mutagenized population of the model legume Lotus japonicus. This approach has resulted in a collection of 21 mutants that exhibit defects at various stages of AM development while maintaining the capacity to form root nodules. These mutants provide a valuable tool to dissect the plant's contribution to symbiosis. We propose a detailed characterization of the genes involved in AM both at the phenotypic and molecular level. For this we will describe the mutant phenotype by microscopy, transcriptional profiling and analysis of AM performance. We will continue the genetic characterization of the mutants by defining rough map positions and complementation groups. At least two of the mutants display defects at early developmental stages, and twelve mutants are impaired during arbuscule development. We propose to identify at least two of the defective genes by map-based cloning. Subsequently, we will functionally characterize the identified genes and their gene products. So far, not a single gene specifically required for AM symbiosis has been identified through forward genetic approaches. The results from this study will therefore provide novel and important insights into the genetic basis of AM.

植物根部和球囊菌门真菌之间的磷酸获取性丛枝菌根（AM）共生具有首要的生态重要性，并具有可持续农业的潜力。我们希望揭示这种共生关系中控制发育和调节的分子机制。为此，我们在豆科植物莲花模型的甲磺酸乙酯（EMS）诱变群体中启动了对 AM 缺陷个体的遗传筛选。这种方法产生了 21 个突变体，它们在 AM 发育的不同阶段表现出缺陷，同时保持形成根瘤的能力。这些突变体提供了一个有价值的工具来剖析植物对共生的贡献。我们提出了在表型和分子水平上对 AM 涉及的基因进行详细表征。为此，我们将通过显微镜、转录谱和 AM 性能分析来描述突变表型。我们将通过定义粗略的图谱位置和互补组来继续突变体的遗传表征。至少有两个突变体在早期发育阶段表现出缺陷，十二个突变体在丛枝发育过程中受到损害。我们建议通过基于图谱的克隆来识别至少两个缺陷基因。随后，我们将对已识别的基因及其基因产物进行功能表征。到目前为止，还没有通过正向遗传方法鉴定出 AM 共生所特异需要的单个基因。因此，这项研究的结果将为 AM 的遗传基础提供新颖且重要的见解。