Functional analysis of Symbiosis Receptor Kinase (SYMRK) mediated signal perception

共生受体激酶 (SYMRK) 介导的信号感知的功能分析

• 批准号: 39030828
• 负责人: Professor Dr. Martin Parniske
• 金额: --
• 依托单位: Institut für Genetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39030828?language=en
• 关键词: Functional; analysis; Symbiosis; Receptor; Kinase

The symbiosis receptor kinase (SYMRK) is required for successful symbiotic interactions between plants and AM fungi, between actinorhiza hosts and Frankia bacteria and between legumes and rhizobia. SYMRK is a membrane bound receptor-like kinase (RLK) with 3 leucine-rich repeat motifs in the extracellular domain and an intracellular protein kinase domain. This project addresses the question how SYMRK mediates developmental reprogramming of plant cells in response to endosymbiotic microorganisms such as arbuscular mycorrhiza (AM) fungi or nitrogen-fixing rhizobia. During the first funding period we have established important tools for the biochemical and microscopical detection of SYMRK in planta and identified endogenous proteins interacting with SYMRK. We will now analyse their functional relevance using reverse genetics. Moreover we aim to discern the subcellular and tissue localization of SYMRK complexes and how symbiotic signalling molecules and/or interactome components influence the spatiotemporal dynamics of SYMRK. We expect this project to provide novel insights into symbiotic signalling mechanisms and plant receptor kinase function in general.

共生受体激酶 (SYMRK) 是植物与 AM 真菌之间、放线菌宿主与 Frankia 细菌之间以及豆科植物与根瘤菌之间成功共生相互作用所必需的。 SYMRK 是一种膜结合受体样激酶 (RLK)，在胞外结构域和胞内蛋白激酶结构域中具有 3 个富含亮氨酸的重复基序。该项目解决了 SYMRK 如何介导植物细胞的发育重编程以响应内共生微生物（例如丛枝菌根 (AM) 真菌或固氮根瘤菌）的问题。在第一个资助期间，我们建立了用于植物中 SYMRK 生化和显微检测的重要工具，并鉴定了与 SYMRK 相互作用的内源蛋白。我们现在将使用反向遗传学分析它们的功能相关性。此外，我们的目标是辨别 SYMRK 复合物的亚细胞和组织定位，以及共生信号分子和/或相互作用组成分如何影响 SYMRK 的时空动态。我们期望这个项目能够为共生信号传导机制和植物受体激酶的一般功能提供新的见解。