Development of contrast agents that image tumor-specific protease for Overhauser effect-enhaced MRI

开发用于奥豪瑟效应增强 MRI 的肿瘤特异性蛋白酶成像造影剂

• 批准号: 23650266
• 负责人: NIIDOME Takuro
• 金额: $ 2.33万
• 依托单位: Kumamoto University (2012-2013)Kyushu University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650266/
• 关键词: 医用・生体画像; バイオイメージング; MRI; オーバーハウザー効果; 腫瘍; プロテアーゼ; オーバーハウザー効果MRI; 電子スピン共鳴; デンドリマー; ニトロキシラジカル; ペプチド; オーバーハウザー効果ＭＲＩ; ＥＳＲ

Overhauser-enhanced MRI (OMRI) is a double resonance technique that uses the presence of paramagnetic agents such as nitroxyl radicals to enhance the signal intensity from nuclear spins by a process known as the Overhauser effect. In this study, dendritic poly(L-lysine) polymers were modified with stable nitroxyl radicals through substrate peptides of a protease specifically expressed in tumors. Cleavage of the peptides by the protease affected the OMRI signal from the radicals. This system could be the basis of a functional tumor imaging technique by OMRI.

奥弗豪瑟增强 MRI (OMRI) 是一种双共振技术，利用硝酰基自由基等顺磁剂的存在，通过称为奥弗豪瑟效应的过程来增强核自旋的信号强度。在这项研究中，通过肿瘤中特异性表达的蛋白酶的底物肽，用稳定的硝酰基自由基修饰树状聚（L-赖氨酸）聚合物。蛋白酶对肽的裂解影响了自由基的 OMRI 信号。该系统可以成为 OMRI 功能性肿瘤成像技术的基础。

项目成果

Rapid and serum-insensitive endocytotic delivery of proteins using biotinylated polymers attached via multivalent hydrophobic anchors

使用通过多价疏水锚附着的生物素化聚合物快速且血清不敏感的内吞递送蛋白质

• 发表时间: 2014
• 作者: K. Tobinaga; C. Li; M. Takeo; M. Matsuda; H. Nagai; T. Niidome; T. Yamamoto; A. Kishimura; T. Mori; Y. Katayama
• 通讯作者: Y. Katayama

Thermal enhancement of gene transfection in tumor cells mediated by the photothermal effect of gold nanorods

金纳米棒光热效应介导的肿瘤细胞基因转染热增强

• 发表时间: 2013
• 作者: Y. Sakamura; M. Yoshiura; H. Tang; T. Mori; Y. Katayama; T. Niidome
• 通讯作者: T. Niidome

PEG-silica-modified gold nanorods that retain their optical properties in tumor tissues

PEG-二氧化硅修饰的金纳米棒在肿瘤组织中保留其光学特性

• DOI: 10.1080/09205063.2013.823073
• 发表时间: 2013
• 作者: Y. Akiyama; Y. Niidome; T. Mori; Y. Katayama; T. Niidome
• 通讯作者: T. Niidome

Branched polyethylenimine-based PKCα-responsive gene carriers

基于分支聚乙烯亚胺的 PKCα 响应基因载体

• DOI: 10.1080/09205063.2013.807459
• 发表时间: 2013
• 作者: Yuta Nakamura; et al.
• 通讯作者: et al.

Sequential release of single-stranded DNAs from gold nanorods triggered by near-infrared light irradiation

近红外光照射触发金纳米棒顺序释放单链 DNA

• 发表时间: 2012
• 作者: H. Fukushima; S. Yamashita; T. Mori; Y. Katayama; T. Niidome
• 通讯作者: T. Niidome