Detection for miRNA dysregulation in CML

检测 CML 中的 miRNA 失调

• 批准号: 23591365
• 负责人: TAKAHASHI Naoto
• 金额: $ 3.49万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591365/
• 关键词: CML; miRNA; miR-200c; Bmi-1; microRNA; 骨髄性白血病; 慢性骨髄性白血病

To detect specific miRNA in cancer stem cell of chromic myelogenious leukemia (CML), we established CML mice (B6) which can develop CML. We got cancer stem cells from B6 mice and examined miRNA expression from KLS population. We found that several miRNA were up-or down regulated in these mice. For instance, down regulation of miR-203,miR-200c, miR-26,miR-21,miR-2105, and miR-181. In this time we are examining targets of these miRNAs. We identified Bmi-1 is possible target of miR-200c in CML stem cells. And we fort her demonstrated that bortezomib was effective for diminishing CML stem cells by targeting both Bmi-1 and miR-200c.

为了检测慢性粒细胞白血病（CML）癌症干细胞中的特异性miRNA，我们建立了能够发展为CML的CML小鼠（B6）。我们从 B6 小鼠身上获取癌症干细胞，并检查了 KLS 群体的 miRNA 表达。我们发现这些小鼠中一些 miRNA 上调或下调。例如，miR-203、miR-200c、miR-26、miR-21、miR-2105 和 miR-181 的下调。这次我们正在研究这些 miRNA 的靶点。我们确定 Bmi-1 是 CML 干细胞中 miR-200c 的可能靶标。我们还证明硼替佐米可以通过靶向 Bmi-1 和 miR-200c 来有效减少 CML 干细胞。

项目成果

Enucleation of human erythroblasts involves non-muscle myosin IIB

人成红细胞的去核涉及非肌肉肌球蛋白 IIB

• 发表时间: 2012
• 影响因子: 20.3
• 作者: Ubukawa K; Guo YM; Takahashi M; Hirokawa M; Michishita Y; Nara M; Tagawa H; Takahashi N; Komatsuda A; Nunomura W; Takakuwa Y; Sawada K
• 通讯作者: Sawada K

A synthetic double-stranded RNA, poly I : C, induces a rapid apoptosis of human CD34(+) cells

合成的双链 RNA，poly I : C，诱导人 CD34( ) 细胞快速凋亡

• 发表时间: 2012
• 影响因子: 2.6
• 作者: Liu J; Guo YM; Hirokawa M; Iwamoto K; Ubukawa K; Michishita Y; Fujishima N; Tagawa H; Takahashi N; Xiao W; Yamashita J; Ohteki T; Sawada K
• 通讯作者: Sawada K

The role of miR-150 in malignant lymphoma

miR-150在恶性淋巴瘤中的作用

• DOI: 10.1038/leu.2011.81
• 发表时间: 2011
• 影响因子: 11.4
• 作者: Waatanabe A; Tagawa H; Takahashi N; et al.
• 通讯作者: et al.

Bortezomib reduces the tumorigenicity of multiple myeloma via downregulation of upregulated targets in clonogenic side population cells

硼替佐米通过下调克隆侧群细胞中上调的靶标来降低多发性骨髓瘤的致瘤性

• 发表时间: 2013
• 作者: Nara M; Teshima K; Watanabe A; Ito M; Iwamoto K; Kitabayashi A; Kume M; Hatano Y; Takahashi N; Iida S; Sawada K; Tagawa H.
• 通讯作者: Tagawa H.