Pathopysiology and treatment in emerging infectious disease, Neonatal TSS-like exanthematous disease, induced by exotoxin from MRSA

MRSA 外毒素诱发的新发传染病、新生儿 TSS 样发疹性疾病的病理生理学和治疗

基本信息

批准号：
12671070
负责人：
TAKAHASHI Naoto
金额：
$ 2.5万
依托单位：
Jichi Medical School (2001)Tokyo Women's Medical University (2000)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671070/
关键词：
MRSA TSST- 1 neonates superantigen NTED

项目摘要

1. According to replies to our questionnaires, neonates fulfilling the clinical criteria of NTED have been observed in 80.5% of major neonatal care units in Japan until 2000. Two hundred five NTED cases were observed in 44 units in 2000.2. Fifty-four among 60 MRSA strains isolated from NTED patients in major neonatal care units in retained TSST-1/SEC genes. One MRSA isolate retained SEB gene. Forty-nine MRSA isolates can be classified in MRSA Clone A of which coagulase type was II, while only one MRSA isolate is classified in Clone B having coagulase typeVII and SEB gene. These clone A MRSA isolates were collected from any area of Japan.3. The down regulation of Vβ2^+ T cells was consistently observed in the very early phase of NTED. We investigated Vβ2 expression in samples of NTED suspected cases from 9 NICUs in Japan. Eight of 9 cases can be estimated to be compatible for the change of Vβ2^+ T cells in NTED.4. The parallel use of the separation of MRSA carriers and MRSA-free neonates, the use of gloves in all cares of infants, and the use of closed tracheal suction system was effective to decrease of the number of MRSA carriers.5. The serum titers of anti-TSST-1 antibody were significantly positive in 65% of Japanese pregnant women and their infants' cord blood. The main subclasses of anti-TSST-1 antibody were IgG1 and IgG4.

1. 根据我们对调查问卷的答复，截至 2000 年，日本 80.5% 的主要新生儿护理单位观察到了符合 NTED 临床标准的新生儿。2000.2 年，44 个单位中观察到了 205 例 NTED 病例，其中 60 个单位中有 54 例。从主要新生儿护理单位的 NTED 患者中分离出的 MRSA 菌株保留了 TSST-1/SEC 基因，其中一株 MRSA 分离株保留了 SEB 基因。 49 株 MRSA 分离株可归入 MRSA 克隆 A，其凝固酶类型为 II，而只有 1 株 MRSA 分离株归入具有凝固酶 VII 型和 SEB 基因的克隆 B，这些克隆 A MRSA 分离株是从日本任何地区收集的。 .3 在 NTED 的早期阶段一致观察到 Vβ2^+ T 细胞的下调，我们研究了来自 9 个 NICU 的 NTED 疑似病例样本中的 Vβ2 表达。日本：9 例中的 8 例估计与 NTED 中 Vβ2^+ T 细胞的变化相符。4. 并行使用 MRSA 携带者和无 MRSA 新生儿的分离，在婴儿的所有护理中使用手套。 5．日本孕妇及其孕妇血清抗TSST-1抗体滴度显着阳性。婴儿脐带血中抗TSST-1抗体的主要亚类是IgG1和IgG4。